Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2755 and GFF2756 overlap by 4 nucleotides GFF2756 and GFF2757 are separated by 36 nucleotides GFF2757 and GFF2758 are separated by 15 nucleotides GFF2758 and GFF2759 overlap by 17 nucleotides
GFF2755 - Ribosyl nicotinamide transporter, PnuC-like, at 187,850 to 188,395
GFF2755
GFF2756 - Ribosylnicotinamide kinase (EC 2.7.1.22), at 188,392 to 189,063
GFF2756
GFF2757 - hypothetical protein, at 189,100 to 190,071
GFF2757
GFF2758 - Transcriptional regulator, RpiR family, at 190,087 to 191,040
GFF2758
GFF2759 - hypothetical protein, at 191,024 to 192,070
GFF2759
Position (kb)
189
190
191 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 188.318 kb on + strand, within GFF2755 at 188.318 kb on + strand, within GFF2755 at 188.319 kb on - strand, within GFF2755 at 188.319 kb on - strand, within GFF2755 at 188.319 kb on - strand, within GFF2755 at 188.319 kb on - strand, within GFF2755 at 188.351 kb on + strand at 188.351 kb on + strand at 188.352 kb on - strand at 188.524 kb on + strand, within GFF2756 at 188.524 kb on + strand, within GFF2756 at 188.524 kb on + strand, within GFF2756 at 188.524 kb on + strand, within GFF2756 at 188.525 kb on - strand, within GFF2756 at 188.672 kb on - strand, within GFF2756 at 188.672 kb on - strand, within GFF2756 at 188.672 kb on - strand, within GFF2756 at 188.704 kb on + strand, within GFF2756 at 188.704 kb on + strand, within GFF2756 at 188.704 kb on + strand, within GFF2756 at 188.705 kb on - strand, within GFF2756 at 188.735 kb on - strand, within GFF2756 at 188.735 kb on - strand, within GFF2756 at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.027 kb on + strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.028 kb on - strand at 189.061 kb on + strand at 189.061 kb on + strand at 189.148 kb on - strand at 189.148 kb on - strand at 189.316 kb on + strand, within GFF2757 at 189.316 kb on + strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.607 kb on + strand, within GFF2757 at 189.655 kb on + strand, within GFF2757 at 189.655 kb on + strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.883 kb on + strand, within GFF2757 at 189.883 kb on + strand, within GFF2757 at 189.883 kb on + strand, within GFF2757 at 189.883 kb on + strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 190.199 kb on + strand, within GFF2758 at 190.199 kb on + strand, within GFF2758 at 190.200 kb on - strand, within GFF2758 at 190.200 kb on - strand, within GFF2758 at 190.358 kb on + strand, within GFF2758 at 190.358 kb on + strand, within GFF2758 at 190.359 kb on - strand, within GFF2758 at 190.478 kb on + strand, within GFF2758 at 190.479 kb on - strand, within GFF2758 at 190.479 kb on - strand, within GFF2758 at 190.727 kb on + strand, within GFF2758 at 190.728 kb on - strand, within GFF2758 at 190.728 kb on - strand, within GFF2758 at 190.794 kb on - strand, within GFF2758
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 188,318 + GFF2755 0.86 -0.1 188,318 + GFF2755 0.86 +1.5 188,319 - GFF2755 0.86 +0.0 188,319 - GFF2755 0.86 +0.4 188,319 - GFF2755 0.86 +0.2 188,319 - GFF2755 0.86 -0.6 188,351 + -0.2 188,351 + -0.7 188,352 - -0.2 188,524 + GFF2756 0.20 +0.8 188,524 + GFF2756 0.20 -0.3 188,524 + GFF2756 0.20 +0.9 188,524 + GFF2756 0.20 +0.7 188,525 - GFF2756 0.20 +1.3 188,672 - GFF2756 0.42 -0.0 188,672 - GFF2756 0.42 -1.0 188,672 - GFF2756 0.42 -0.0 188,704 + GFF2756 0.46 -0.7 188,704 + GFF2756 0.46 +0.0 188,704 + GFF2756 0.46 -0.4 188,705 - GFF2756 0.47 +0.0 188,735 - GFF2756 0.51 +0.8 188,735 - GFF2756 0.51 -0.2 189,027 + -0.2 189,027 + -0.1 189,027 + -0.7 189,027 + -1.5 189,027 + +1.0 189,027 + -0.4 189,027 + -0.4 189,027 + -0.0 189,027 + -0.7 189,027 + -0.3 189,027 + -0.3 189,027 + +0.5 189,027 + -0.2 189,027 + +0.2 189,028 - -0.8 189,028 - +1.5 189,028 - -0.0 189,028 - -0.3 189,028 - -0.5 189,028 - +0.0 189,028 - -0.5 189,028 - +0.4 189,028 - +0.9 189,028 - +0.6 189,028 - +0.2 189,028 - -1.0 189,028 - +0.0 189,028 - -0.2 189,028 - -0.1 189,028 - +0.0 189,028 - +0.2 189,028 - +0.3 189,028 - -1.4 189,061 + -0.1 189,061 + +0.2 189,148 - +0.4 189,148 - +0.1 189,316 + GFF2757 0.22 +0.4 189,316 + GFF2757 0.22 -0.5 189,317 - GFF2757 0.22 +0.1 189,317 - GFF2757 0.22 -0.0 189,317 - GFF2757 0.22 +1.5 189,317 - GFF2757 0.22 +0.9 189,317 - GFF2757 0.22 -0.3 189,607 + GFF2757 0.52 -3.0 189,655 + GFF2757 0.57 -0.7 189,655 + GFF2757 0.57 +0.4 189,656 - GFF2757 0.57 -1.5 189,656 - GFF2757 0.57 +0.8 189,656 - GFF2757 0.57 -0.6 189,656 - GFF2757 0.57 +1.5 189,656 - GFF2757 0.57 +0.7 189,883 + GFF2757 0.81 +0.5 189,883 + GFF2757 0.81 +0.1 189,883 + GFF2757 0.81 -0.4 189,883 + GFF2757 0.81 +0.5 189,884 - GFF2757 0.81 -1.0 189,884 - GFF2757 0.81 -1.0 189,884 - GFF2757 0.81 +0.2 189,884 - GFF2757 0.81 -0.3 189,884 - GFF2757 0.81 -0.1 189,884 - GFF2757 0.81 +0.2 189,884 - GFF2757 0.81 -1.8 189,884 - GFF2757 0.81 +0.7 190,199 + GFF2758 0.12 -1.0 190,199 + GFF2758 0.12 -0.6 190,200 - GFF2758 0.12 -0.0 190,200 - GFF2758 0.12 -1.0 190,358 + GFF2758 0.28 -0.7 190,358 + GFF2758 0.28 +0.3 190,359 - GFF2758 0.29 +0.0 190,478 + GFF2758 0.41 -1.4 190,479 - GFF2758 0.41 +1.5 190,479 - GFF2758 0.41 +0.1 190,727 + GFF2758 0.67 +0.7 190,728 - GFF2758 0.67 -1.2 190,728 - GFF2758 0.67 -1.5 190,794 - GFF2758 0.74 -0.1
Or see this region's nucleotide sequence