Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2747 and GFF2748 are separated by 183 nucleotides GFF2748 and GFF2749 are separated by 2 nucleotides GFF2749 and GFF2750 overlap by 1 nucleotides
GFF2747 - 17 kDa surface antigen, at 181,076 to 181,576
GFF2747
GFF2748 - Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23), at 181,760 to 182,206
GFF2748
GFF2749 - hypothetical protein, at 182,209 to 182,685
GFF2749
GFF2750 - Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5), at 182,685 to 183,908
GFF2750
Position (kb)
182
183 Strain fitness (log2 ratio)
-2
-1
0
1 at 181.343 kb on + strand, within GFF2747 at 181.344 kb on - strand, within GFF2747 at 181.417 kb on - strand, within GFF2747 at 181.425 kb on + strand, within GFF2747 at 181.425 kb on + strand, within GFF2747 at 181.426 kb on - strand, within GFF2747 at 181.426 kb on - strand, within GFF2747 at 181.440 kb on + strand, within GFF2747 at 181.489 kb on - strand, within GFF2747 at 181.725 kb on - strand at 182.210 kb on + strand at 182.211 kb on - strand at 182.211 kb on - strand at 182.211 kb on - strand at 182.211 kb on - strand at 182.211 kb on - strand at 182.633 kb on + strand, within GFF2749 at 182.633 kb on + strand, within GFF2749 at 182.633 kb on + strand, within GFF2749 at 182.633 kb on + strand, within GFF2749 at 182.633 kb on + strand, within GFF2749 at 182.633 kb on + strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.634 kb on - strand, within GFF2749 at 182.702 kb on + strand at 182.702 kb on + strand at 182.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 181,343 + GFF2747 0.53 +0.4 181,344 - GFF2747 0.53 -0.4 181,417 - GFF2747 0.68 -0.0 181,425 + GFF2747 0.70 -0.3 181,425 + GFF2747 0.70 -0.3 181,426 - GFF2747 0.70 -0.2 181,426 - GFF2747 0.70 -0.6 181,440 + GFF2747 0.73 -0.0 181,489 - GFF2747 0.82 -0.8 181,725 - +0.2 182,210 + +1.1 182,211 - -0.4 182,211 - +0.2 182,211 - -0.9 182,211 - -0.9 182,211 - +0.2 182,633 + GFF2749 0.89 +1.1 182,633 + GFF2749 0.89 -1.5 182,633 + GFF2749 0.89 -0.2 182,633 + GFF2749 0.89 -0.5 182,633 + GFF2749 0.89 -0.2 182,633 + GFF2749 0.89 -0.5 182,634 - GFF2749 0.89 +0.1 182,634 - GFF2749 0.89 -0.4 182,634 - GFF2749 0.89 -0.4 182,634 - GFF2749 0.89 +0.3 182,634 - GFF2749 0.89 -0.9 182,634 - GFF2749 0.89 -0.5 182,634 - GFF2749 0.89 -0.5 182,634 - GFF2749 0.89 +0.2 182,634 - GFF2749 0.89 -2.2 182,634 - GFF2749 0.89 -0.8 182,634 - GFF2749 0.89 -1.2 182,702 + -0.4 182,702 + -0.2 182,703 - -0.0
Or see this region's nucleotide sequence