Strain Fitness in Variovorax sp. SCN45 around GFF2498

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2497 and GFF2498 are separated by 30 nucleotidesGFF2498 and GFF2499 overlap by 4 nucleotidesGFF2499 and GFF2500 overlap by 4 nucleotides GFF2497 - Protein translocase subunit SecA, at 555,561 to 558,347 GFF2497 GFF2498 - Glutamate N-acetyltransferase (EC 2.3.1.35) @ N-acetylglutamate synthase (EC 2.3.1.1), at 558,378 to 559,607 GFF2498 GFF2499 - ATP/GTP-binding protein, at 559,604 to 560,479 GFF2499 GFF2500 - Mutator MutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-), at 560,476 to 560,910 GFF2500 Position (kb) 558 559 560Strain fitness (log2 ratio) -2 -1 0 1at 558.159 kb on + strandat 558.159 kb on + strandat 558.159 kb on + strandat 558.159 kb on + strandat 558.159 kb on + strandat 558.159 kb on + strandat 558.160 kb on - strandat 558.160 kb on - strandat 558.160 kb on - strandat 558.318 kb on + strandat 558.318 kb on + strandat 558.318 kb on + strandat 558.319 kb on - strandat 558.319 kb on - strandat 558.319 kb on - strandat 558.319 kb on - strandat 558.319 kb on - strandat 558.319 kb on - strandat 558.319 kb on - strandat 558.319 kb on - strandat 558.362 kb on + strandat 558.362 kb on + strandat 558.362 kb on + strandat 558.362 kb on + strandat 558.362 kb on + strandat 558.362 kb on + strandat 558.363 kb on - strandat 558.363 kb on - strandat 558.363 kb on - strandat 559.287 kb on + strand, within GFF2498at 559.287 kb on + strand, within GFF2498at 559.287 kb on + strand, within GFF2498at 559.288 kb on - strand, within GFF2498at 559.326 kb on + strand, within GFF2498at 559.326 kb on + strand, within GFF2498at 559.326 kb on + strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.372 kb on - strand, within GFF2498at 559.462 kb on - strand, within GFF2498at 559.575 kb on + strandat 559.575 kb on + strandat 559.576 kb on - strandat 559.576 kb on - strandat 559.576 kb on - strandat 559.576 kb on - strandat 559.576 kb on - strandat 559.937 kb on + strand, within GFF2499at 559.937 kb on + strand, within GFF2499at 559.938 kb on - strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.082 kb on - strand, within GFF2499at 560.082 kb on - strand, within GFF2499at 560.082 kb on - strand, within GFF2499at 560.082 kb on - strand, within GFF2499at 560.144 kb on + strand, within GFF2499at 560.145 kb on - strand, within GFF2499at 560.145 kb on - strand, within GFF2499at 560.145 kb on - strand, within GFF2499at 560.180 kb on + strand, within GFF2499at 560.181 kb on - strand, within GFF2499at 560.181 kb on - strand, within GFF2499at 560.201 kb on + strand, within GFF2499at 560.201 kb on + strand, within GFF2499at 560.202 kb on - strand, within GFF2499at 560.202 kb on - strand, within GFF2499at 560.294 kb on + strand, within GFF2499at 560.294 kb on + strand, within GFF2499at 560.295 kb on - strand, within GFF2499at 560.295 kb on - strand, within GFF2499at 560.295 kb on - strand, within GFF2499at 560.295 kb on - strand, within GFF2499at 560.315 kb on + strand, within GFF2499at 560.315 kb on + strand, within GFF2499at 560.316 kb on - strand, within GFF2499at 560.316 kb on - strand, within GFF2499at 560.438 kb on + strandat 560.439 kb on - strandat 560.456 kb on + strandat 560.457 kb on - strandat 560.457 kb on - strandat 560.575 kb on + strand, within GFF2500at 560.575 kb on + strand, within GFF2500at 560.576 kb on - strand, within GFF2500at 560.584 kb on + strand, within GFF2500at 560.585 kb on - strand, within GFF2500

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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558,159 + -1.0
558,159 + -0.2
558,159 + -0.2
558,159 + +0.6
558,159 + +0.1
558,159 + -0.6
558,160 - -0.9
558,160 - -0.1
558,160 - +1.0
558,318 + -0.3
558,318 + -2.2
558,318 + -0.4
558,319 - -0.6
558,319 - -0.9
558,319 - +1.1
558,319 - -0.6
558,319 - -0.4
558,319 - -0.4
558,319 - +0.6
558,319 - -0.5
558,362 + -0.8
558,362 + -0.8
558,362 + +1.3
558,362 + +0.8
558,362 + +0.1
558,362 + -0.4
558,363 - +0.6
558,363 - -0.1
558,363 - -0.1
559,287 + GFF2498 0.74 +0.9
559,287 + GFF2498 0.74 -0.2
559,287 + GFF2498 0.74 +0.1
559,288 - GFF2498 0.74 +0.5
559,326 + GFF2498 0.77 +1.4
559,326 + GFF2498 0.77 -0.9
559,326 + GFF2498 0.77 +1.1
559,327 - GFF2498 0.77 +0.4
559,327 - GFF2498 0.77 -0.3
559,327 - GFF2498 0.77 +0.2
559,327 - GFF2498 0.77 -0.6
559,327 - GFF2498 0.77 -0.0
559,372 - GFF2498 0.81 +0.5
559,462 - GFF2498 0.88 -0.3
559,575 + -0.9
559,575 + -0.1
559,576 - +0.2
559,576 - -0.4
559,576 - +0.1
559,576 - -0.2
559,576 - -0.7
559,937 + GFF2499 0.38 +0.7
559,937 + GFF2499 0.38 +1.5
559,938 - GFF2499 0.38 -0.5
560,081 + GFF2499 0.54 +0.1
560,081 + GFF2499 0.54 +0.1
560,081 + GFF2499 0.54 -0.7
560,081 + GFF2499 0.54 -0.3
560,081 + GFF2499 0.54 -1.2
560,082 - GFF2499 0.55 -0.9
560,082 - GFF2499 0.55 -0.0
560,082 - GFF2499 0.55 -0.0
560,082 - GFF2499 0.55 +1.5
560,144 + GFF2499 0.62 +0.5
560,145 - GFF2499 0.62 -0.7
560,145 - GFF2499 0.62 -0.0
560,145 - GFF2499 0.62 -0.5
560,180 + GFF2499 0.66 -0.2
560,181 - GFF2499 0.66 -1.1
560,181 - GFF2499 0.66 -0.1
560,201 + GFF2499 0.68 +0.0
560,201 + GFF2499 0.68 +0.2
560,202 - GFF2499 0.68 -0.7
560,202 - GFF2499 0.68 +0.0
560,294 + GFF2499 0.79 -0.9
560,294 + GFF2499 0.79 -0.8
560,295 - GFF2499 0.79 -2.3
560,295 - GFF2499 0.79 -0.4
560,295 - GFF2499 0.79 +0.4
560,295 - GFF2499 0.79 -1.3
560,315 + GFF2499 0.81 -0.7
560,315 + GFF2499 0.81 -0.5
560,316 - GFF2499 0.81 +0.1
560,316 - GFF2499 0.81 -0.1
560,438 + -0.8
560,439 - +1.0
560,456 + +0.3
560,457 - +0.4
560,457 - -0.3
560,575 + GFF2500 0.23 -0.1
560,575 + GFF2500 0.23 -0.9
560,576 - GFF2500 0.23 -1.3
560,584 + GFF2500 0.25 -0.6
560,585 - GFF2500 0.25 -0.5

Or see this region's nucleotide sequence