Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2402 and GFF2403 are separated by 54 nucleotides GFF2403 and GFF2404 are separated by 121 nucleotides GFF2404 and GFF2405 are separated by 152 nucleotides GFF2405 and GFF2406 are separated by 21 nucleotides
GFF2402 - hypothetical protein, at 455,291 to 455,752
GFF2402
GFF2403 - CHAP domain containing protein, at 455,807 to 456,418
GFF2403
GFF2404 - Hypothetical protein with Rieske (2Fe-2S) region, at 456,540 to 457,622
GFF2404
GFF2405 - Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12), at 457,775 to 458,284
GFF2405
GFF2406 - DipZ protein, at 458,306 to 460,159
GFF2406
Position (kb)
456
457
458 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 455.564 kb on + strand, within GFF2402 at 455.571 kb on + strand, within GFF2402 at 455.571 kb on + strand, within GFF2402 at 455.571 kb on + strand, within GFF2402 at 455.572 kb on - strand, within GFF2402 at 455.572 kb on - strand, within GFF2402 at 455.572 kb on - strand, within GFF2402 at 455.667 kb on + strand, within GFF2402 at 455.835 kb on + strand at 455.836 kb on - strand at 455.966 kb on + strand, within GFF2403 at 455.966 kb on + strand, within GFF2403 at 455.966 kb on + strand, within GFF2403 at 455.967 kb on - strand, within GFF2403 at 456.017 kb on + strand, within GFF2403 at 456.017 kb on + strand, within GFF2403 at 456.035 kb on + strand, within GFF2403 at 456.035 kb on + strand, within GFF2403 at 456.036 kb on - strand, within GFF2403 at 456.036 kb on - strand, within GFF2403 at 456.062 kb on + strand, within GFF2403 at 456.065 kb on + strand, within GFF2403 at 456.065 kb on + strand, within GFF2403 at 456.119 kb on + strand, within GFF2403 at 456.120 kb on - strand, within GFF2403 at 456.120 kb on - strand, within GFF2403 at 456.401 kb on - strand at 456.445 kb on + strand at 456.539 kb on + strand at 456.744 kb on + strand, within GFF2404 at 456.745 kb on - strand, within GFF2404 at 456.745 kb on - strand, within GFF2404 at 456.846 kb on + strand, within GFF2404 at 456.847 kb on - strand, within GFF2404 at 457.173 kb on + strand, within GFF2404 at 457.173 kb on + strand, within GFF2404 at 457.174 kb on - strand, within GFF2404 at 457.212 kb on + strand, within GFF2404 at 457.233 kb on + strand, within GFF2404 at 457.233 kb on + strand, within GFF2404 at 457.354 kb on + strand, within GFF2404 at 457.354 kb on + strand, within GFF2404 at 457.354 kb on + strand, within GFF2404 at 457.355 kb on - strand, within GFF2404 at 457.355 kb on - strand, within GFF2404 at 457.355 kb on - strand, within GFF2404 at 457.355 kb on - strand, within GFF2404 at 457.449 kb on + strand, within GFF2404 at 457.449 kb on + strand, within GFF2404 at 457.449 kb on + strand, within GFF2404 at 457.450 kb on - strand, within GFF2404 at 457.450 kb on - strand, within GFF2404 at 457.450 kb on - strand, within GFF2404 at 457.710 kb on + strand at 457.717 kb on + strand at 457.717 kb on + strand at 457.718 kb on - strand at 457.718 kb on - strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.728 kb on - strand at 457.728 kb on - strand at 457.728 kb on - strand at 457.728 kb on - strand at 457.728 kb on - strand at 457.729 kb on + strand at 457.729 kb on + strand at 457.730 kb on - strand at 457.730 kb on - strand at 457.731 kb on + strand at 457.731 kb on + strand at 457.731 kb on + strand at 457.731 kb on + strand at 457.731 kb on + strand at 457.732 kb on - strand at 457.732 kb on - strand at 457.946 kb on + strand, within GFF2405 at 457.947 kb on - strand, within GFF2405 at 457.947 kb on - strand, within GFF2405 at 458.237 kb on + strand at 458.328 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 455,564 + GFF2402 0.59 +0.8 455,571 + GFF2402 0.61 +0.5 455,571 + GFF2402 0.61 +0.2 455,571 + GFF2402 0.61 +1.3 455,572 - GFF2402 0.61 +0.3 455,572 - GFF2402 0.61 +0.1 455,572 - GFF2402 0.61 -0.2 455,667 + GFF2402 0.81 -0.3 455,835 + -1.3 455,836 - +0.7 455,966 + GFF2403 0.26 +0.2 455,966 + GFF2403 0.26 +0.1 455,966 + GFF2403 0.26 -0.7 455,967 - GFF2403 0.26 -1.4 456,017 + GFF2403 0.34 +1.0 456,017 + GFF2403 0.34 -0.2 456,035 + GFF2403 0.37 -0.4 456,035 + GFF2403 0.37 -0.3 456,036 - GFF2403 0.37 -0.1 456,036 - GFF2403 0.37 -1.0 456,062 + GFF2403 0.42 -1.0 456,065 + GFF2403 0.42 -0.1 456,065 + GFF2403 0.42 -2.2 456,119 + GFF2403 0.51 +0.9 456,120 - GFF2403 0.51 -1.0 456,120 - GFF2403 0.51 +0.3 456,401 - -1.1 456,445 + +0.9 456,539 + -1.0 456,744 + GFF2404 0.19 -0.4 456,745 - GFF2404 0.19 -0.1 456,745 - GFF2404 0.19 +0.8 456,846 + GFF2404 0.28 -1.2 456,847 - GFF2404 0.28 +0.2 457,173 + GFF2404 0.58 -0.1 457,173 + GFF2404 0.58 +0.4 457,174 - GFF2404 0.59 -0.1 457,212 + GFF2404 0.62 -0.1 457,233 + GFF2404 0.64 -1.1 457,233 + GFF2404 0.64 -0.8 457,354 + GFF2404 0.75 +0.7 457,354 + GFF2404 0.75 -0.6 457,354 + GFF2404 0.75 -0.3 457,355 - GFF2404 0.75 -0.1 457,355 - GFF2404 0.75 +0.1 457,355 - GFF2404 0.75 +0.6 457,355 - GFF2404 0.75 +1.7 457,449 + GFF2404 0.84 -0.5 457,449 + GFF2404 0.84 +1.7 457,449 + GFF2404 0.84 -0.9 457,450 - GFF2404 0.84 +0.1 457,450 - GFF2404 0.84 -2.8 457,450 - GFF2404 0.84 -1.4 457,710 + -0.1 457,717 + -1.5 457,717 + -1.1 457,718 - +0.0 457,718 - +0.4 457,727 + -0.6 457,727 + -2.7 457,727 + +1.1 457,727 + -0.5 457,727 + +0.2 457,727 + -1.9 457,727 + -0.8 457,727 + -0.1 457,727 + -1.5 457,727 + -0.8 457,727 + +0.2 457,727 + -0.8 457,728 - -0.6 457,728 - -0.1 457,728 - -0.8 457,728 - -0.5 457,728 - -1.1 457,729 + -0.8 457,729 + +1.0 457,730 - -0.1 457,730 - -0.5 457,731 + -1.3 457,731 + -0.7 457,731 + -0.4 457,731 + +0.7 457,731 + +0.4 457,732 - -0.8 457,732 - -0.9 457,946 + GFF2405 0.34 +0.7 457,947 - GFF2405 0.34 +0.7 457,947 - GFF2405 0.34 -0.2 458,237 + -0.4 458,328 - -0.1
Or see this region's nucleotide sequence