Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2354 and GFF2355 are separated by 28 nucleotides GFF2355 and GFF2356 are separated by 6 nucleotides GFF2356 and GFF2357 overlap by 4 nucleotides
GFF2354 - FIG00555615: hypothetical protein, at 404,426 to 406,387
GFF2354
GFF2355 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 406,416 to 407,462
GFF2355
GFF2356 - RNase adapter protein RapZ, at 407,469 to 408,347
GFF2356
GFF2357 - DNA repair protein RecN, at 408,344 to 410,026
GFF2357
Position (kb)
406
407
408 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 405.625 kb on + strand, within GFF2354 at 405.626 kb on - strand, within GFF2354 at 405.626 kb on - strand, within GFF2354 at 405.626 kb on - strand, within GFF2354 at 405.725 kb on + strand, within GFF2354 at 405.725 kb on + strand, within GFF2354 at 405.725 kb on + strand, within GFF2354 at 405.725 kb on + strand, within GFF2354 at 405.725 kb on + strand, within GFF2354 at 405.726 kb on - strand, within GFF2354 at 405.726 kb on - strand, within GFF2354 at 405.726 kb on - strand, within GFF2354 at 405.726 kb on - strand, within GFF2354 at 405.726 kb on - strand, within GFF2354 at 405.726 kb on - strand, within GFF2354 at 405.821 kb on + strand, within GFF2354 at 405.821 kb on + strand, within GFF2354 at 405.821 kb on + strand, within GFF2354 at 405.821 kb on + strand, within GFF2354 at 405.821 kb on + strand, within GFF2354 at 405.822 kb on - strand, within GFF2354 at 405.822 kb on - strand, within GFF2354 at 406.016 kb on + strand, within GFF2354 at 406.016 kb on + strand, within GFF2354 at 406.017 kb on - strand, within GFF2354 at 406.017 kb on - strand, within GFF2354 at 406.017 kb on - strand, within GFF2354 at 406.017 kb on - strand, within GFF2354 at 406.017 kb on - strand, within GFF2354 at 406.569 kb on + strand, within GFF2355 at 406.570 kb on - strand, within GFF2355 at 406.662 kb on + strand, within GFF2355 at 406.662 kb on + strand, within GFF2355 at 406.662 kb on + strand, within GFF2355 at 406.665 kb on + strand, within GFF2355 at 406.665 kb on + strand, within GFF2355 at 406.665 kb on + strand, within GFF2355 at 406.666 kb on - strand, within GFF2355 at 406.968 kb on + strand, within GFF2355 at 406.968 kb on + strand, within GFF2355 at 406.968 kb on + strand, within GFF2355 at 406.968 kb on + strand, within GFF2355 at 406.968 kb on + strand, within GFF2355 at 406.969 kb on - strand, within GFF2355 at 406.969 kb on - strand, within GFF2355 at 406.969 kb on - strand, within GFF2355 at 407.460 kb on + strand at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.744 kb on - strand, within GFF2356 at 407.744 kb on - strand, within GFF2356 at 407.744 kb on - strand, within GFF2356 at 407.779 kb on + strand, within GFF2356 at 407.779 kb on + strand, within GFF2356 at 407.779 kb on + strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.872 kb on + strand, within GFF2356 at 407.872 kb on + strand, within GFF2356 at 407.872 kb on + strand, within GFF2356 at 407.872 kb on + strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 408.049 kb on + strand, within GFF2356 at 408.049 kb on + strand, within GFF2356 at 408.262 kb on + strand at 408.262 kb on + strand at 408.262 kb on + strand at 408.262 kb on + strand at 408.262 kb on + strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.265 kb on + strand at 408.266 kb on - strand at 408.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 405,625 + GFF2354 0.61 +0.0 405,626 - GFF2354 0.61 -0.1 405,626 - GFF2354 0.61 +0.6 405,626 - GFF2354 0.61 -0.7 405,725 + GFF2354 0.66 +0.3 405,725 + GFF2354 0.66 -0.1 405,725 + GFF2354 0.66 +0.0 405,725 + GFF2354 0.66 +0.3 405,725 + GFF2354 0.66 +0.2 405,726 - GFF2354 0.66 +0.9 405,726 - GFF2354 0.66 -0.4 405,726 - GFF2354 0.66 -0.6 405,726 - GFF2354 0.66 -0.9 405,726 - GFF2354 0.66 +0.3 405,726 - GFF2354 0.66 +0.0 405,821 + GFF2354 0.71 -0.2 405,821 + GFF2354 0.71 -0.5 405,821 + GFF2354 0.71 +0.4 405,821 + GFF2354 0.71 +1.0 405,821 + GFF2354 0.71 +0.5 405,822 - GFF2354 0.71 +0.7 405,822 - GFF2354 0.71 -0.1 406,016 + GFF2354 0.81 +0.4 406,016 + GFF2354 0.81 +0.9 406,017 - GFF2354 0.81 -0.3 406,017 - GFF2354 0.81 +0.1 406,017 - GFF2354 0.81 -0.6 406,017 - GFF2354 0.81 -0.1 406,017 - GFF2354 0.81 -0.2 406,569 + GFF2355 0.15 -0.0 406,570 - GFF2355 0.15 -0.5 406,662 + GFF2355 0.23 -0.0 406,662 + GFF2355 0.23 -0.4 406,662 + GFF2355 0.23 +1.1 406,665 + GFF2355 0.24 +0.1 406,665 + GFF2355 0.24 +0.0 406,665 + GFF2355 0.24 +0.4 406,666 - GFF2355 0.24 -0.5 406,968 + GFF2355 0.53 +0.3 406,968 + GFF2355 0.53 +0.8 406,968 + GFF2355 0.53 -0.4 406,968 + GFF2355 0.53 +0.4 406,968 + GFF2355 0.53 -1.4 406,969 - GFF2355 0.53 +0.0 406,969 - GFF2355 0.53 +0.6 406,969 - GFF2355 0.53 -0.9 407,460 + -0.5 407,596 + GFF2356 0.14 -0.4 407,596 + GFF2356 0.14 -0.5 407,596 + GFF2356 0.14 +0.3 407,596 + GFF2356 0.14 +1.2 407,596 + GFF2356 0.14 -1.2 407,596 + GFF2356 0.14 -0.6 407,597 - GFF2356 0.15 -1.3 407,597 - GFF2356 0.15 -0.8 407,597 - GFF2356 0.15 -1.0 407,597 - GFF2356 0.15 -0.6 407,597 - GFF2356 0.15 +0.9 407,659 + GFF2356 0.22 -1.0 407,659 + GFF2356 0.22 -0.5 407,659 + GFF2356 0.22 -1.1 407,659 + GFF2356 0.22 -0.5 407,659 + GFF2356 0.22 +0.1 407,659 + GFF2356 0.22 -1.0 407,660 - GFF2356 0.22 -0.1 407,660 - GFF2356 0.22 -0.3 407,660 - GFF2356 0.22 -0.3 407,660 - GFF2356 0.22 +0.3 407,660 - GFF2356 0.22 -0.4 407,660 - GFF2356 0.22 -1.3 407,743 + GFF2356 0.31 +0.6 407,743 + GFF2356 0.31 -1.9 407,743 + GFF2356 0.31 +1.7 407,743 + GFF2356 0.31 -1.4 407,743 + GFF2356 0.31 +0.3 407,743 + GFF2356 0.31 +0.1 407,743 + GFF2356 0.31 -0.1 407,744 - GFF2356 0.31 -0.3 407,744 - GFF2356 0.31 -0.6 407,744 - GFF2356 0.31 -0.6 407,779 + GFF2356 0.35 +0.1 407,779 + GFF2356 0.35 -0.3 407,779 + GFF2356 0.35 +0.3 407,780 - GFF2356 0.35 +0.7 407,780 - GFF2356 0.35 +0.1 407,780 - GFF2356 0.35 -2.1 407,780 - GFF2356 0.35 -0.7 407,780 - GFF2356 0.35 -1.1 407,872 + GFF2356 0.46 -1.6 407,872 + GFF2356 0.46 -0.9 407,872 + GFF2356 0.46 -1.2 407,872 + GFF2356 0.46 -0.6 407,873 - GFF2356 0.46 -1.9 407,873 - GFF2356 0.46 -2.1 407,873 - GFF2356 0.46 -0.7 407,873 - GFF2356 0.46 -0.9 407,873 - GFF2356 0.46 -0.5 407,873 - GFF2356 0.46 -0.4 407,873 - GFF2356 0.46 -1.3 407,873 - GFF2356 0.46 -1.6 407,873 - GFF2356 0.46 -1.6 407,873 - GFF2356 0.46 -0.4 407,873 - GFF2356 0.46 -0.2 407,873 - GFF2356 0.46 +0.1 408,049 + GFF2356 0.66 +0.6 408,049 + GFF2356 0.66 -0.5 408,262 + -1.1 408,262 + -1.3 408,262 + -3.0 408,262 + -1.1 408,262 + -0.0 408,263 - -1.3 408,263 - -1.2 408,263 - -0.0 408,263 - +1.1 408,263 - -0.7 408,263 - -0.4 408,265 + -1.5 408,266 - -0.7 408,266 - -0.3
Or see this region's nucleotide sequence