Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2265 and GFF2266 are separated by 5 nucleotides GFF2266 and GFF2267 are separated by 90 nucleotides GFF2267 and GFF2268 are separated by 100 nucleotides GFF2268 and GFF2269 are separated by 70 nucleotides
GFF2265 - Catechol 1,2-dioxygenase (EC 1.13.11.1), at 320,523 to 321,356
GFF2265
GFF2266 - FIG00441126: hypothetical protein, at 321,362 to 321,967
GFF2266
GFF2267 - Integral membrane protein, at 322,058 to 322,816
GFF2267
GFF2268 - Protein of unknown function DUF1428, at 322,917 to 323,276
GFF2268
GFF2269 - Uncharacterized MFS-type transporter, at 323,347 to 324,813
GFF2269
Position (kb)
322
323 Strain fitness (log2 ratio)
-2
-1
0
1 at 321.093 kb on + strand, within GFF2265 at 321.195 kb on + strand, within GFF2265 at 321.506 kb on + strand, within GFF2266 at 321.506 kb on + strand, within GFF2266 at 321.689 kb on + strand, within GFF2266 at 321.690 kb on - strand, within GFF2266 at 321.746 kb on + strand, within GFF2266 at 321.746 kb on + strand, within GFF2266 at 321.746 kb on + strand, within GFF2266 at 321.747 kb on - strand, within GFF2266 at 321.747 kb on - strand, within GFF2266 at 321.845 kb on + strand, within GFF2266 at 321.845 kb on + strand, within GFF2266 at 321.845 kb on + strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.846 kb on - strand, within GFF2266 at 321.920 kb on + strand at 321.966 kb on - strand at 322.008 kb on + strand at 322.085 kb on + strand at 322.085 kb on + strand at 322.086 kb on - strand at 322.086 kb on - strand at 322.086 kb on - strand at 322.091 kb on + strand at 322.091 kb on + strand at 322.092 kb on - strand at 322.092 kb on - strand at 322.535 kb on + strand, within GFF2267 at 322.535 kb on + strand, within GFF2267 at 322.535 kb on + strand, within GFF2267 at 322.535 kb on + strand, within GFF2267 at 322.535 kb on + strand, within GFF2267 at 322.536 kb on - strand, within GFF2267 at 322.536 kb on - strand, within GFF2267 at 322.536 kb on - strand, within GFF2267 at 322.536 kb on - strand, within GFF2267 at 322.536 kb on - strand, within GFF2267 at 322.536 kb on - strand, within GFF2267 at 322.536 kb on - strand, within GFF2267 at 322.536 kb on - strand, within GFF2267 at 322.586 kb on + strand, within GFF2267 at 322.586 kb on + strand, within GFF2267 at 322.586 kb on + strand, within GFF2267 at 322.587 kb on - strand, within GFF2267 at 322.587 kb on - strand, within GFF2267 at 322.748 kb on + strand at 322.749 kb on - strand at 322.749 kb on - strand at 322.797 kb on - strand at 322.807 kb on + strand at 322.808 kb on - strand at 322.808 kb on - strand at 322.808 kb on - strand at 322.808 kb on - strand at 323.056 kb on + strand, within GFF2268 at 323.056 kb on + strand, within GFF2268 at 323.056 kb on + strand, within GFF2268 at 323.056 kb on + strand, within GFF2268 at 323.056 kb on + strand, within GFF2268 at 323.056 kb on + strand, within GFF2268 at 323.057 kb on - strand, within GFF2268 at 323.057 kb on - strand, within GFF2268 at 323.057 kb on - strand, within GFF2268 at 323.057 kb on - strand, within GFF2268 at 323.057 kb on - strand, within GFF2268 at 323.161 kb on + strand, within GFF2268 at 323.161 kb on + strand, within GFF2268 at 323.162 kb on - strand, within GFF2268 at 323.177 kb on - strand, within GFF2268 at 323.262 kb on + strand at 323.262 kb on + strand at 323.262 kb on + strand at 323.262 kb on + strand at 323.262 kb on + strand at 323.263 kb on - strand at 323.266 kb on + strand at 323.266 kb on + strand at 323.266 kb on + strand at 323.267 kb on - strand at 323.267 kb on - strand at 323.267 kb on - strand at 323.489 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 321,093 + GFF2265 0.68 -0.0 321,195 + GFF2265 0.81 +0.6 321,506 + GFF2266 0.24 -0.3 321,506 + GFF2266 0.24 +0.2 321,689 + GFF2266 0.54 +1.3 321,690 - GFF2266 0.54 -0.4 321,746 + GFF2266 0.63 +0.3 321,746 + GFF2266 0.63 -0.4 321,746 + GFF2266 0.63 -0.0 321,747 - GFF2266 0.64 -0.8 321,747 - GFF2266 0.64 -0.2 321,845 + GFF2266 0.80 -0.1 321,845 + GFF2266 0.80 -0.5 321,845 + GFF2266 0.80 +0.1 321,846 - GFF2266 0.80 -1.8 321,846 - GFF2266 0.80 -2.2 321,846 - GFF2266 0.80 -0.4 321,846 - GFF2266 0.80 +0.2 321,846 - GFF2266 0.80 +1.5 321,846 - GFF2266 0.80 +1.7 321,846 - GFF2266 0.80 +0.1 321,846 - GFF2266 0.80 +1.0 321,846 - GFF2266 0.80 -1.6 321,846 - GFF2266 0.80 +0.1 321,920 + +0.1 321,966 - +0.7 322,008 + -0.5 322,085 + -0.5 322,085 + +0.0 322,086 - +1.2 322,086 - -0.5 322,086 - -2.6 322,091 + -0.6 322,091 + +0.9 322,092 - +0.2 322,092 - -0.3 322,535 + GFF2267 0.63 +0.6 322,535 + GFF2267 0.63 -0.0 322,535 + GFF2267 0.63 -1.2 322,535 + GFF2267 0.63 +0.5 322,535 + GFF2267 0.63 +0.4 322,536 - GFF2267 0.63 +0.6 322,536 - GFF2267 0.63 +0.1 322,536 - GFF2267 0.63 +0.2 322,536 - GFF2267 0.63 -0.6 322,536 - GFF2267 0.63 -0.1 322,536 - GFF2267 0.63 +0.3 322,536 - GFF2267 0.63 -2.2 322,536 - GFF2267 0.63 +0.4 322,586 + GFF2267 0.70 -0.4 322,586 + GFF2267 0.70 +1.2 322,586 + GFF2267 0.70 +1.3 322,587 - GFF2267 0.70 -0.7 322,587 - GFF2267 0.70 -0.1 322,748 + -1.1 322,749 - -0.0 322,749 - -1.0 322,797 - +0.6 322,807 + +0.7 322,808 - +0.4 322,808 - -0.7 322,808 - -0.4 322,808 - -0.2 323,056 + GFF2268 0.39 +0.2 323,056 + GFF2268 0.39 -0.1 323,056 + GFF2268 0.39 -1.4 323,056 + GFF2268 0.39 +0.5 323,056 + GFF2268 0.39 -0.1 323,056 + GFF2268 0.39 -2.2 323,057 - GFF2268 0.39 -0.4 323,057 - GFF2268 0.39 -0.4 323,057 - GFF2268 0.39 +0.4 323,057 - GFF2268 0.39 +0.7 323,057 - GFF2268 0.39 -0.4 323,161 + GFF2268 0.68 -0.8 323,161 + GFF2268 0.68 +0.4 323,162 - GFF2268 0.68 +0.7 323,177 - GFF2268 0.72 +0.2 323,262 + -0.5 323,262 + +0.0 323,262 + -0.7 323,262 + -0.4 323,262 + +0.2 323,263 - -0.4 323,266 + +1.1 323,266 + -0.2 323,266 + +0.2 323,267 - -0.7 323,267 - -0.0 323,267 - +0.2 323,489 + -1.1
Or see this region's nucleotide sequence