Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2173 and GFF2174 overlap by 4 nucleotides GFF2174 and GFF2175 are separated by 19 nucleotides GFF2175 and GFF2176 are separated by 262 nucleotides GFF2176 and GFF2177 are separated by 35 nucleotides GFF2177 and GFF2178 are separated by 12 nucleotides
GFF2173 - no description, at 227,315 to 228,502
GFF2173
GFF2174 - hypothetical protein, at 228,499 to 229,212
GFF2174
GFF2175 - hypothetical protein, at 229,232 to 230,764
GFF2175
GFF2176 - no description, at 231,027 to 231,290
GFF2176
GFF2177 - no description, at 231,326 to 231,643
GFF2177
GFF2178 - Methyl-accepting chemotaxis sensor/transducer protein, at 231,656 to 233,323
GFF2178
Position (kb)
229
230
231 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 228.338 kb on + strand, within GFF2173 at 228.339 kb on - strand, within GFF2173 at 228.339 kb on - strand, within GFF2173 at 228.827 kb on + strand, within GFF2174 at 229.281 kb on + strand at 229.282 kb on - strand at 229.500 kb on + strand, within GFF2175 at 229.500 kb on + strand, within GFF2175 at 229.501 kb on - strand, within GFF2175 at 229.501 kb on - strand, within GFF2175 at 229.602 kb on + strand, within GFF2175 at 229.602 kb on + strand, within GFF2175 at 229.603 kb on - strand, within GFF2175 at 229.603 kb on - strand, within GFF2175 at 229.603 kb on - strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.641 kb on + strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.642 kb on - strand, within GFF2175 at 229.726 kb on - strand, within GFF2175 at 229.726 kb on - strand, within GFF2175 at 229.766 kb on - strand, within GFF2175 at 230.465 kb on + strand, within GFF2175 at 230.466 kb on - strand, within GFF2175 at 230.466 kb on - strand, within GFF2175 at 230.466 kb on - strand, within GFF2175 at 230.466 kb on - strand, within GFF2175 at 230.720 kb on + strand at 230.879 kb on + strand at 230.880 kb on - strand at 230.880 kb on - strand at 231.109 kb on - strand, within GFF2176 at 231.288 kb on + strand at 231.289 kb on - strand at 231.289 kb on - strand at 231.291 kb on + strand at 231.449 kb on + strand, within GFF2177 at 231.450 kb on - strand, within GFF2177 at 231.450 kb on - strand, within GFF2177 at 231.450 kb on - strand, within GFF2177
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 228,338 + GFF2173 0.86 +1.2 228,339 - GFF2173 0.86 +0.2 228,339 - GFF2173 0.86 +1.2 228,827 + GFF2174 0.46 +0.6 229,281 + -0.4 229,282 - +0.2 229,500 + GFF2175 0.17 +0.3 229,500 + GFF2175 0.17 +0.6 229,501 - GFF2175 0.18 +0.2 229,501 - GFF2175 0.18 -0.1 229,602 + GFF2175 0.24 -0.0 229,602 + GFF2175 0.24 -1.1 229,603 - GFF2175 0.24 +0.5 229,603 - GFF2175 0.24 +1.5 229,603 - GFF2175 0.24 -0.0 229,641 + GFF2175 0.27 -0.6 229,641 + GFF2175 0.27 -0.5 229,641 + GFF2175 0.27 -0.8 229,641 + GFF2175 0.27 -0.4 229,641 + GFF2175 0.27 -0.0 229,641 + GFF2175 0.27 -0.5 229,641 + GFF2175 0.27 +0.0 229,641 + GFF2175 0.27 +1.5 229,641 + GFF2175 0.27 -0.5 229,641 + GFF2175 0.27 +0.0 229,642 - GFF2175 0.27 -1.1 229,642 - GFF2175 0.27 -1.2 229,642 - GFF2175 0.27 +0.3 229,642 - GFF2175 0.27 -0.6 229,642 - GFF2175 0.27 +0.3 229,642 - GFF2175 0.27 -1.2 229,642 - GFF2175 0.27 +0.2 229,642 - GFF2175 0.27 -0.1 229,642 - GFF2175 0.27 +3.3 229,642 - GFF2175 0.27 -1.9 229,726 - GFF2175 0.32 +0.0 229,726 - GFF2175 0.32 +0.3 229,766 - GFF2175 0.35 +0.1 230,465 + GFF2175 0.80 +0.0 230,466 - GFF2175 0.80 +0.4 230,466 - GFF2175 0.80 -0.2 230,466 - GFF2175 0.80 -0.3 230,466 - GFF2175 0.80 +0.4 230,720 + +0.2 230,879 + -0.2 230,880 - +0.3 230,880 - +0.8 231,109 - GFF2176 0.31 -0.4 231,288 + -0.5 231,289 - -0.8 231,289 - -0.8 231,291 + -1.7 231,449 + GFF2177 0.39 +0.8 231,450 - GFF2177 0.39 +0.4 231,450 - GFF2177 0.39 -0.2 231,450 - GFF2177 0.39 -0.3
Or see this region's nucleotide sequence