Strain Fitness in Variovorax sp. SCN45 around GFF2161

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2159 and GFF2160 overlap by 1 nucleotidesGFF2160 and GFF2161 overlap by 4 nucleotidesGFF2161 and GFF2162 are separated by 46 nucleotides GFF2159 - Pyrimidine operon regulatory protein PyrR, at 211,966 to 212,463 GFF2159 GFF2160 - Aspartate carbamoyltransferase (EC 2.1.3.2), at 212,463 to 213,425 GFF2160 GFF2161 - Dihydroorotase (EC 3.5.2.3), at 213,422 to 214,711 GFF2161 GFF2162 - Acyl-CoA:1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 214,758 to 215,504 GFF2162 Position (kb) 213 214 215Strain fitness (log2 ratio) -2 -1 0 1 2at 212.461 kb on + strandat 212.461 kb on + strandat 212.461 kb on + strandat 212.461 kb on + strandat 212.461 kb on + strandat 212.461 kb on + strandat 212.461 kb on + strandat 212.461 kb on + strandat 212.461 kb on + strandat 212.461 kb on + strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.462 kb on - strandat 212.469 kb on + strandat 212.469 kb on + strandat 212.469 kb on + strandat 212.469 kb on + strandat 212.469 kb on + strandat 212.470 kb on - strandat 212.470 kb on - strandat 212.470 kb on - strandat 212.470 kb on - strandat 212.838 kb on + strand, within GFF2160at 212.838 kb on + strand, within GFF2160at 212.839 kb on - strand, within GFF2160at 212.931 kb on + strand, within GFF2160at 212.931 kb on + strand, within GFF2160at 212.931 kb on + strand, within GFF2160at 212.931 kb on + strand, within GFF2160at 212.931 kb on + strand, within GFF2160at 212.931 kb on + strand, within GFF2160at 212.931 kb on + strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.932 kb on - strand, within GFF2160at 212.946 kb on + strand, within GFF2160at 212.946 kb on + strand, within GFF2160at 212.947 kb on - strand, within GFF2160at 213.413 kb on + strandat 213.414 kb on - strandat 213.632 kb on + strand, within GFF2161at 213.932 kb on + strand, within GFF2161at 213.932 kb on + strand, within GFF2161at 213.932 kb on + strand, within GFF2161at 213.933 kb on - strand, within GFF2161at 213.933 kb on - strand, within GFF2161at 213.951 kb on - strand, within GFF2161at 214.236 kb on - strand, within GFF2161at 214.548 kb on + strand, within GFF2161at 214.548 kb on + strand, within GFF2161at 214.548 kb on - strand, within GFF2161at 214.549 kb on - strand, within GFF2161at 214.549 kb on - strand, within GFF2161at 214.549 kb on - strand, within GFF2161at 214.655 kb on - strandat 214.678 kb on + strandat 214.678 kb on + strandat 214.678 kb on + strandat 214.679 kb on - strandat 214.679 kb on - strandat 214.679 kb on - strandat 214.679 kb on - strandat 214.712 kb on + strandat 214.713 kb on - strandat 214.713 kb on - strandat 215.097 kb on + strand, within GFF2162at 215.097 kb on + strand, within GFF2162at 215.097 kb on + strand, within GFF2162at 215.097 kb on + strand, within GFF2162at 215.097 kb on + strand, within GFF2162at 215.097 kb on + strand, within GFF2162at 215.097 kb on + strand, within GFF2162at 215.097 kb on + strand, within GFF2162at 215.098 kb on - strand, within GFF2162at 215.098 kb on - strand, within GFF2162at 215.098 kb on - strand, within GFF2162at 215.098 kb on - strand, within GFF2162at 215.254 kb on + strand, within GFF2162at 215.255 kb on - strand, within GFF2162at 215.255 kb on - strand, within GFF2162at 215.334 kb on + strand, within GFF2162at 215.334 kb on + strand, within GFF2162at 215.334 kb on + strand, within GFF2162at 215.335 kb on - strand, within GFF2162at 215.335 kb on - strand, within GFF2162at 215.335 kb on - strand, within GFF2162at 215.335 kb on - strand, within GFF2162at 215.588 kb on + strandat 215.588 kb on + strandat 215.589 kb on - strandat 215.589 kb on - strandat 215.600 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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212,461 + +0.1
212,461 + +0.0
212,461 + +0.4
212,461 + -0.6
212,461 + +2.3
212,461 + -0.8
212,461 + -0.3
212,461 + +0.0
212,461 + -0.5
212,461 + -0.2
212,462 - -0.2
212,462 - -1.0
212,462 - -0.1
212,462 - +0.7
212,462 - -0.4
212,462 - +1.5
212,462 - -1.6
212,462 - -1.0
212,462 - +0.8
212,462 - -0.9
212,462 - -0.4
212,462 - -1.2
212,469 + +0.2
212,469 + +0.0
212,469 + +1.1
212,469 + -0.1
212,469 + +1.0
212,470 - +0.7
212,470 - -0.9
212,470 - -0.3
212,470 - -0.3
212,838 + GFF2160 0.39 +0.2
212,838 + GFF2160 0.39 -0.2
212,839 - GFF2160 0.39 -0.3
212,931 + GFF2160 0.49 +0.4
212,931 + GFF2160 0.49 +1.4
212,931 + GFF2160 0.49 +1.0
212,931 + GFF2160 0.49 +0.2
212,931 + GFF2160 0.49 +0.2
212,931 + GFF2160 0.49 -0.7
212,931 + GFF2160 0.49 -0.8
212,932 - GFF2160 0.49 -0.2
212,932 - GFF2160 0.49 -0.1
212,932 - GFF2160 0.49 +0.1
212,932 - GFF2160 0.49 -0.6
212,932 - GFF2160 0.49 -0.3
212,932 - GFF2160 0.49 -0.0
212,932 - GFF2160 0.49 +0.5
212,932 - GFF2160 0.49 -0.4
212,932 - GFF2160 0.49 +0.2
212,932 - GFF2160 0.49 +0.7
212,932 - GFF2160 0.49 -0.4
212,932 - GFF2160 0.49 +0.9
212,932 - GFF2160 0.49 +0.4
212,946 + GFF2160 0.50 -0.4
212,946 + GFF2160 0.50 +0.7
212,947 - GFF2160 0.50 -0.0
213,413 + -0.3
213,414 - -0.8
213,632 + GFF2161 0.16 +1.7
213,932 + GFF2161 0.40 -0.6
213,932 + GFF2161 0.40 -1.4
213,932 + GFF2161 0.40 -0.1
213,933 - GFF2161 0.40 +0.7
213,933 - GFF2161 0.40 -1.3
213,951 - GFF2161 0.41 -1.2
214,236 - GFF2161 0.63 +0.1
214,548 + GFF2161 0.87 -1.4
214,548 + GFF2161 0.87 -1.1
214,548 - GFF2161 0.87 -1.3
214,549 - GFF2161 0.87 -0.6
214,549 - GFF2161 0.87 -0.7
214,549 - GFF2161 0.87 +0.6
214,655 - +0.4
214,678 + -0.1
214,678 + +0.0
214,678 + -1.6
214,679 - +0.5
214,679 - +0.4
214,679 - +1.2
214,679 - +0.0
214,712 + +0.1
214,713 - +0.5
214,713 - +1.7
215,097 + GFF2162 0.45 -1.3
215,097 + GFF2162 0.45 -1.8
215,097 + GFF2162 0.45 -1.5
215,097 + GFF2162 0.45 -0.0
215,097 + GFF2162 0.45 -1.3
215,097 + GFF2162 0.45 -0.4
215,097 + GFF2162 0.45 +0.9
215,097 + GFF2162 0.45 +0.5
215,098 - GFF2162 0.46 -0.1
215,098 - GFF2162 0.46 -1.0
215,098 - GFF2162 0.46 +0.7
215,098 - GFF2162 0.46 -1.8
215,254 + GFF2162 0.66 -0.8
215,255 - GFF2162 0.67 -0.1
215,255 - GFF2162 0.67 -0.3
215,334 + GFF2162 0.77 -1.3
215,334 + GFF2162 0.77 -0.1
215,334 + GFF2162 0.77 -1.1
215,335 - GFF2162 0.77 -0.3
215,335 - GFF2162 0.77 +0.2
215,335 - GFF2162 0.77 -0.4
215,335 - GFF2162 0.77 -0.1
215,588 + -0.3
215,588 + -1.0
215,589 - -0.7
215,589 - -1.7
215,600 - -0.8

Or see this region's nucleotide sequence