Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2125 and GFF2126 are separated by 321 nucleotides GFF2126 and GFF2127 overlap by 4 nucleotides GFF2127 and GFF2128 are separated by 30 nucleotides GFF2128 and GFF2129 are separated by 96 nucleotides
GFF2125 - hypothetical protein, at 175,001 to 175,351
GFF2125
GFF2126 - hypothetical protein, at 175,673 to 176,338
GFF2126
GFF2127 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 176,335 to 177,612
GFF2127
GFF2128 - Enoyl-CoA hydratase (EC 4.2.1.17), at 177,643 to 178,422
GFF2128
GFF2129 - small multidrug resistance family (SMR) protein, at 178,519 to 178,854
GFF2129
Position (kb) 176
177
178 Strain fitness (log2 ratio) -2
-1
0
1
2 at 175.392 kb on + strand at 175.392 kb on + strand at 175.444 kb on + strand at 175.444 kb on + strand at 175.444 kb on + strand at 175.445 kb on - strand at 175.445 kb on - strand at 175.648 kb on + strand at 175.648 kb on + strand at 175.648 kb on + strand at 175.649 kb on - strand at 175.790 kb on + strand, within GFF2126 at 175.791 kb on - strand, within GFF2126 at 176.783 kb on + strand, within GFF2127 at 176.926 kb on - strand, within GFF2127 at 176.926 kb on - strand, within GFF2127 at 176.926 kb on - strand, within GFF2127 at 176.926 kb on - strand, within GFF2127 at 177.062 kb on + strand, within GFF2127 at 177.062 kb on + strand, within GFF2127 at 177.063 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.196 kb on + strand, within GFF2127 at 177.196 kb on + strand, within GFF2127 at 177.473 kb on - strand, within GFF2127 at 177.821 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.203 kb on - strand, within GFF2128 at 178.203 kb on - strand, within GFF2128 at 178.203 kb on - strand, within GFF2128 at 178.203 kb on - strand, within GFF2128 at 178.415 kb on + strand at 178.415 kb on + strand at 178.416 kb on - strand at 178.461 kb on - strand at 178.461 kb on - strand at 178.462 kb on + strand at 178.462 kb on + strand at 178.462 kb on + strand at 178.463 kb on - strand at 178.506 kb on + strand at 178.507 kb on - strand at 178.507 kb on - strand at 178.507 kb on - strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.532 kb on - strand at 178.537 kb on + strand at 178.537 kb on + strand at 178.538 kb on - strand at 178.538 kb on - strand at 178.538 kb on - strand at 178.538 kb on - strand at 178.538 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 175,392 + -0.3 175,392 + +0.1 175,444 + +0.4 175,444 + +1.3 175,444 + -1.6 175,445 - -0.6 175,445 - -0.3 175,648 + -1.2 175,648 + -0.2 175,648 + -0.6 175,649 - +0.0 175,790 + GFF2126 0.18 -2.1 175,791 - GFF2126 0.18 -0.2 176,783 + GFF2127 0.35 -1.0 176,926 - GFF2127 0.46 -0.2 176,926 - GFF2127 0.46 -0.7 176,926 - GFF2127 0.46 -0.4 176,926 - GFF2127 0.46 -0.6 177,062 + GFF2127 0.57 -0.8 177,062 + GFF2127 0.57 -0.3 177,063 - GFF2127 0.57 -0.8 177,086 - GFF2127 0.59 +0.1 177,086 - GFF2127 0.59 -1.1 177,086 - GFF2127 0.59 -0.6 177,086 - GFF2127 0.59 -0.6 177,086 - GFF2127 0.59 -0.7 177,196 + GFF2127 0.67 -0.9 177,196 + GFF2127 0.67 +0.8 177,473 - GFF2127 0.89 +1.2 177,821 + GFF2128 0.23 -1.2 178,172 + GFF2128 0.68 -1.7 178,172 + GFF2128 0.68 -2.4 178,172 + GFF2128 0.68 -0.2 178,172 + GFF2128 0.68 -2.5 178,172 + GFF2128 0.68 -0.6 178,172 + GFF2128 0.68 -2.1 178,172 + GFF2128 0.68 -0.7 178,184 + GFF2128 0.69 -2.6 178,184 + GFF2128 0.69 -2.0 178,184 + GFF2128 0.69 -2.7 178,184 + GFF2128 0.69 -0.8 178,184 + GFF2128 0.69 -1.2 178,185 - GFF2128 0.69 -1.4 178,185 - GFF2128 0.69 -1.6 178,185 - GFF2128 0.69 -1.2 178,185 - GFF2128 0.69 -0.2 178,185 - GFF2128 0.69 -2.3 178,202 + GFF2128 0.72 -0.9 178,202 + GFF2128 0.72 -2.1 178,202 + GFF2128 0.72 -2.0 178,202 + GFF2128 0.72 -2.7 178,202 + GFF2128 0.72 -1.1 178,203 - GFF2128 0.72 -0.9 178,203 - GFF2128 0.72 -1.9 178,203 - GFF2128 0.72 -1.7 178,203 - GFF2128 0.72 -1.0 178,415 + -0.2 178,415 + -1.5 178,416 - -0.7 178,461 - -1.3 178,461 - -0.8 178,462 + -0.2 178,462 + -0.2 178,462 + -0.7 178,463 - +0.7 178,506 + +0.2 178,507 - -0.2 178,507 - -0.2 178,507 - -0.7 178,531 + -0.1 178,531 + -1.0 178,531 + +0.1 178,531 + -0.2 178,531 + +2.2 178,531 + +0.0 178,531 + -0.2 178,531 + -0.3 178,531 + +0.0 178,532 - +0.0 178,537 + -0.3 178,537 + -0.3 178,538 - +0.8 178,538 - +0.1 178,538 - +0.2 178,538 - +0.4 178,538 - +0.2
Or see this region's nucleotide sequence