Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2081 and GFF2082 are separated by 273 nucleotides GFF2082 and GFF2083 are separated by 9 nucleotides GFF2083 and GFF2084 overlap by 4 nucleotides
GFF2081 - no description, at 129,381 to 130,445
GFF2081
GFF2082 - transcriptional regulator, Crp/Fnr family, at 130,719 to 131,351
GFF2082
GFF2083 - putative periplasmic membrane protein, at 131,361 to 131,963
GFF2083
GFF2084 - Phosphatidate cytidylyltransferase (EC 2.7.7.41), at 131,960 to 132,913
GFF2084
Position (kb)
130
131
132 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 129.736 kb on + strand, within GFF2081 at 129.757 kb on + strand, within GFF2081 at 129.757 kb on + strand, within GFF2081 at 129.758 kb on - strand, within GFF2081 at 129.823 kb on + strand, within GFF2081 at 129.824 kb on - strand, within GFF2081 at 129.832 kb on + strand, within GFF2081 at 129.832 kb on + strand, within GFF2081 at 129.832 kb on + strand, within GFF2081 at 129.832 kb on + strand, within GFF2081 at 129.832 kb on + strand, within GFF2081 at 129.833 kb on - strand, within GFF2081 at 129.833 kb on - strand, within GFF2081 at 129.833 kb on - strand, within GFF2081 at 129.833 kb on - strand, within GFF2081 at 129.833 kb on - strand, within GFF2081 at 129.833 kb on - strand, within GFF2081 at 129.835 kb on + strand, within GFF2081 at 129.836 kb on - strand, within GFF2081 at 129.868 kb on + strand, within GFF2081 at 129.869 kb on - strand, within GFF2081 at 129.916 kb on + strand, within GFF2081 at 130.061 kb on - strand, within GFF2081 at 130.084 kb on + strand, within GFF2081 at 130.084 kb on + strand, within GFF2081 at 130.085 kb on - strand, within GFF2081 at 130.085 kb on - strand, within GFF2081 at 130.099 kb on + strand, within GFF2081 at 130.099 kb on + strand, within GFF2081 at 130.099 kb on + strand, within GFF2081 at 130.100 kb on - strand, within GFF2081 at 130.100 kb on - strand, within GFF2081 at 130.348 kb on + strand at 130.348 kb on + strand at 130.348 kb on + strand at 130.348 kb on + strand at 130.348 kb on + strand at 130.348 kb on + strand at 130.348 kb on + strand at 130.348 kb on + strand at 130.349 kb on - strand at 130.349 kb on - strand at 130.349 kb on - strand at 130.349 kb on - strand at 130.349 kb on - strand at 130.367 kb on - strand at 130.367 kb on - strand at 130.367 kb on - strand at 130.367 kb on - strand at 130.405 kb on + strand at 130.524 kb on + strand at 130.529 kb on + strand at 130.529 kb on + strand at 130.743 kb on + strand at 130.743 kb on + strand at 130.743 kb on + strand at 130.743 kb on + strand at 130.851 kb on + strand, within GFF2082 at 130.851 kb on + strand, within GFF2082 at 130.852 kb on - strand, within GFF2082 at 130.852 kb on - strand, within GFF2082 at 130.852 kb on - strand, within GFF2082 at 130.924 kb on - strand, within GFF2082 at 130.930 kb on - strand, within GFF2082 at 131.133 kb on + strand, within GFF2082 at 131.134 kb on - strand, within GFF2082 at 131.134 kb on - strand, within GFF2082 at 131.134 kb on - strand, within GFF2082 at 131.134 kb on - strand, within GFF2082 at 131.293 kb on - strand at 131.293 kb on - strand at 131.293 kb on - strand at 131.293 kb on - strand at 131.572 kb on + strand, within GFF2083 at 131.728 kb on + strand, within GFF2083 at 131.728 kb on + strand, within GFF2083 at 131.728 kb on + strand, within GFF2083 at 131.729 kb on - strand, within GFF2083 at 131.729 kb on - strand, within GFF2083 at 131.729 kb on - strand, within GFF2083 at 131.976 kb on + strand at 131.994 kb on + strand at 131.994 kb on + strand at 131.995 kb on - strand at 132.186 kb on + strand, within GFF2084 at 132.187 kb on - strand, within GFF2084 at 132.303 kb on + strand, within GFF2084 at 132.303 kb on + strand, within GFF2084 at 132.303 kb on + strand, within GFF2084 at 132.303 kb on + strand, within GFF2084 at 132.304 kb on - strand, within GFF2084 at 132.304 kb on - strand, within GFF2084 at 132.304 kb on - strand, within GFF2084 at 132.304 kb on - strand, within GFF2084 at 132.304 kb on - strand, within GFF2084
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 129,736 + GFF2081 0.33 -1.2 129,757 + GFF2081 0.35 -0.7 129,757 + GFF2081 0.35 -2.3 129,758 - GFF2081 0.35 -0.6 129,823 + GFF2081 0.42 +1.3 129,824 - GFF2081 0.42 -0.5 129,832 + GFF2081 0.42 -0.1 129,832 + GFF2081 0.42 +0.3 129,832 + GFF2081 0.42 -0.5 129,832 + GFF2081 0.42 +1.2 129,832 + GFF2081 0.42 -1.1 129,833 - GFF2081 0.42 +0.8 129,833 - GFF2081 0.42 +0.1 129,833 - GFF2081 0.42 +0.0 129,833 - GFF2081 0.42 +0.4 129,833 - GFF2081 0.42 -1.0 129,833 - GFF2081 0.42 -0.2 129,835 + GFF2081 0.43 -0.2 129,836 - GFF2081 0.43 +0.1 129,868 + GFF2081 0.46 +0.3 129,869 - GFF2081 0.46 -0.6 129,916 + GFF2081 0.50 -1.3 130,061 - GFF2081 0.64 +0.8 130,084 + GFF2081 0.66 -0.7 130,084 + GFF2081 0.66 +0.5 130,085 - GFF2081 0.66 +1.4 130,085 - GFF2081 0.66 -0.9 130,099 + GFF2081 0.67 -0.9 130,099 + GFF2081 0.67 +0.5 130,099 + GFF2081 0.67 -0.2 130,100 - GFF2081 0.68 -0.3 130,100 - GFF2081 0.68 -0.0 130,348 + +1.8 130,348 + -0.8 130,348 + +2.8 130,348 + -0.3 130,348 + +0.0 130,348 + -1.5 130,348 + -0.7 130,348 + -0.5 130,349 - +0.8 130,349 - -1.6 130,349 - -0.5 130,349 - +1.0 130,349 - -1.6 130,367 - +0.2 130,367 - -0.2 130,367 - +1.4 130,367 - +0.9 130,405 + -0.1 130,524 + -0.9 130,529 + -0.5 130,529 + -0.6 130,743 + +1.1 130,743 + -0.3 130,743 + -0.3 130,743 + +0.2 130,851 + GFF2082 0.21 -0.9 130,851 + GFF2082 0.21 -1.0 130,852 - GFF2082 0.21 -1.4 130,852 - GFF2082 0.21 -0.0 130,852 - GFF2082 0.21 -0.1 130,924 - GFF2082 0.32 -0.5 130,930 - GFF2082 0.33 -0.4 131,133 + GFF2082 0.65 +0.2 131,134 - GFF2082 0.66 -1.4 131,134 - GFF2082 0.66 -0.2 131,134 - GFF2082 0.66 +0.5 131,134 - GFF2082 0.66 -0.7 131,293 - +0.3 131,293 - +0.6 131,293 - +0.0 131,293 - -0.8 131,572 + GFF2083 0.35 -0.2 131,728 + GFF2083 0.61 -0.6 131,728 + GFF2083 0.61 -0.1 131,728 + GFF2083 0.61 -0.5 131,729 - GFF2083 0.61 -0.6 131,729 - GFF2083 0.61 -0.0 131,729 - GFF2083 0.61 -0.8 131,976 + -0.4 131,994 + +0.7 131,994 + -1.4 131,995 - +0.1 132,186 + GFF2084 0.24 +1.0 132,187 - GFF2084 0.24 -0.8 132,303 + GFF2084 0.36 -0.0 132,303 + GFF2084 0.36 -1.2 132,303 + GFF2084 0.36 +0.2 132,303 + GFF2084 0.36 +1.8 132,304 - GFF2084 0.36 -1.3 132,304 - GFF2084 0.36 -0.2 132,304 - GFF2084 0.36 -0.6 132,304 - GFF2084 0.36 -1.6 132,304 - GFF2084 0.36 +2.2
Or see this region's nucleotide sequence