Strain Fitness in Variovorax sp. SCN45 around GFF2017

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2015 and GFF2016 are separated by 6 nucleotidesGFF2016 and GFF2017 are separated by 81 nucleotidesGFF2017 and GFF2018 are separated by 93 nucleotidesGFF2018 and GFF2019 overlap by 4 nucleotides GFF2015 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 60,341 to 60,961 GFF2015 GFF2016 - hypothetical protein, at 60,968 to 61,282 GFF2016 GFF2017 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 61,364 to 62,284 GFF2017 GFF2018 - Transcriptional regulator, IclR family, at 62,378 to 63,127 GFF2018 GFF2019 - FIG00976956: hypothetical protein, at 63,124 to 63,519 GFF2019 Position (kb) 61 62 63Strain fitness (log2 ratio) -2 -1 0 1 2at 60.389 kb on + strandat 60.390 kb on - strandat 60.390 kb on - strandat 60.390 kb on - strandat 60.569 kb on + strand, within GFF2015at 60.569 kb on + strand, within GFF2015at 60.569 kb on + strand, within GFF2015at 60.570 kb on - strand, within GFF2015at 60.570 kb on - strand, within GFF2015at 60.570 kb on - strand, within GFF2015at 60.902 kb on + strandat 60.902 kb on + strandat 60.902 kb on + strandat 61.017 kb on + strand, within GFF2016at 61.017 kb on + strand, within GFF2016at 61.017 kb on + strand, within GFF2016at 61.017 kb on + strand, within GFF2016at 61.018 kb on - strand, within GFF2016at 61.018 kb on - strand, within GFF2016at 61.018 kb on - strand, within GFF2016at 61.018 kb on - strand, within GFF2016at 61.018 kb on - strand, within GFF2016at 61.113 kb on + strand, within GFF2016at 61.113 kb on + strand, within GFF2016at 61.113 kb on + strand, within GFF2016at 61.113 kb on + strand, within GFF2016at 61.113 kb on + strand, within GFF2016at 61.113 kb on + strand, within GFF2016at 61.113 kb on + strand, within GFF2016at 61.113 kb on + strand, within GFF2016at 61.113 kb on + strand, within GFF2016at 61.114 kb on - strand, within GFF2016at 61.114 kb on - strand, within GFF2016at 61.114 kb on - strand, within GFF2016at 61.114 kb on - strand, within GFF2016at 61.114 kb on - strand, within GFF2016at 61.114 kb on - strand, within GFF2016at 61.114 kb on - strand, within GFF2016at 61.114 kb on - strand, within GFF2016at 61.164 kb on + strand, within GFF2016at 61.164 kb on + strand, within GFF2016at 61.164 kb on + strand, within GFF2016at 61.164 kb on + strand, within GFF2016at 61.164 kb on + strand, within GFF2016at 61.164 kb on + strand, within GFF2016at 61.164 kb on + strand, within GFF2016at 61.165 kb on - strand, within GFF2016at 61.165 kb on - strand, within GFF2016at 61.165 kb on - strand, within GFF2016at 61.165 kb on - strand, within GFF2016at 61.165 kb on - strand, within GFF2016at 61.165 kb on - strand, within GFF2016at 61.221 kb on + strand, within GFF2016at 61.221 kb on + strand, within GFF2016at 61.221 kb on + strand, within GFF2016at 61.222 kb on - strand, within GFF2016at 61.224 kb on + strand, within GFF2016at 61.224 kb on + strand, within GFF2016at 61.224 kb on + strand, within GFF2016at 61.224 kb on + strand, within GFF2016at 61.225 kb on - strand, within GFF2016at 61.225 kb on - strand, within GFF2016at 61.225 kb on - strand, within GFF2016at 61.225 kb on - strand, within GFF2016at 61.225 kb on - strand, within GFF2016at 61.225 kb on - strand, within GFF2016at 61.225 kb on - strand, within GFF2016at 61.254 kb on + strandat 61.255 kb on - strandat 61.260 kb on + strandat 61.260 kb on + strandat 61.260 kb on + strandat 61.260 kb on + strandat 61.260 kb on + strandat 61.260 kb on + strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.261 kb on - strandat 61.316 kb on + strandat 61.316 kb on + strandat 61.317 kb on - strandat 61.317 kb on - strandat 61.317 kb on - strandat 61.317 kb on - strandat 61.317 kb on - strandat 61.317 kb on - strandat 61.351 kb on - strandat 61.351 kb on - strandat 61.379 kb on + strandat 61.649 kb on + strand, within GFF2017at 61.649 kb on + strand, within GFF2017at 61.649 kb on + strand, within GFF2017at 61.650 kb on - strand, within GFF2017at 61.650 kb on - strand, within GFF2017at 61.650 kb on - strand, within GFF2017at 61.650 kb on - strand, within GFF2017at 61.695 kb on - strand, within GFF2017at 61.695 kb on - strand, within GFF2017at 61.727 kb on + strand, within GFF2017at 62.007 kb on - strand, within GFF2017at 62.007 kb on - strand, within GFF2017at 62.007 kb on - strand, within GFF2017at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.528 kb on + strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.529 kb on - strand, within GFF2018at 62.534 kb on + strand, within GFF2018at 62.534 kb on + strand, within GFF2018at 62.534 kb on + strand, within GFF2018at 62.534 kb on + strand, within GFF2018at 62.534 kb on + strand, within GFF2018at 62.534 kb on + strand, within GFF2018at 62.534 kb on + strand, within GFF2018at 62.535 kb on - strand, within GFF2018at 62.535 kb on - strand, within GFF2018at 62.535 kb on - strand, within GFF2018at 62.535 kb on - strand, within GFF2018at 62.558 kb on + strand, within GFF2018at 62.558 kb on + strand, within GFF2018at 62.559 kb on - strand, within GFF2018at 62.559 kb on - strand, within GFF2018at 62.559 kb on - strand, within GFF2018at 62.562 kb on - strand, within GFF2018at 62.562 kb on - strand, within GFF2018at 62.711 kb on + strand, within GFF2018at 62.711 kb on + strand, within GFF2018at 62.711 kb on + strand, within GFF2018at 62.711 kb on + strand, within GFF2018at 62.711 kb on + strand, within GFF2018at 62.711 kb on + strand, within GFF2018at 62.712 kb on - strand, within GFF2018at 62.747 kb on + strand, within GFF2018at 62.747 kb on + strand, within GFF2018at 63.011 kb on + strand, within GFF2018at 63.011 kb on + strand, within GFF2018at 63.011 kb on + strand, within GFF2018at 63.011 kb on + strand, within GFF2018at 63.012 kb on - strand, within GFF2018at 63.012 kb on - strand, within GFF2018at 63.012 kb on - strand, within GFF2018at 63.012 kb on - strand, within GFF2018at 63.113 kb on + strandat 63.151 kb on + strandat 63.152 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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60,389 + -1.6
60,390 - -0.1
60,390 - -0.3
60,390 - -0.4
60,569 + GFF2015 0.37 -0.6
60,569 + GFF2015 0.37 +0.4
60,569 + GFF2015 0.37 -0.2
60,570 - GFF2015 0.37 +0.3
60,570 - GFF2015 0.37 -0.5
60,570 - GFF2015 0.37 -0.5
60,902 + -0.5
60,902 + -0.7
60,902 + -0.7
61,017 + GFF2016 0.16 +0.5
61,017 + GFF2016 0.16 -0.8
61,017 + GFF2016 0.16 +1.8
61,017 + GFF2016 0.16 -0.6
61,018 - GFF2016 0.16 -0.8
61,018 - GFF2016 0.16 -0.8
61,018 - GFF2016 0.16 +0.0
61,018 - GFF2016 0.16 +0.2
61,018 - GFF2016 0.16 -0.5
61,113 + GFF2016 0.46 +0.1
61,113 + GFF2016 0.46 -0.4
61,113 + GFF2016 0.46 +0.2
61,113 + GFF2016 0.46 -0.1
61,113 + GFF2016 0.46 -0.3
61,113 + GFF2016 0.46 -0.5
61,113 + GFF2016 0.46 -0.3
61,113 + GFF2016 0.46 -0.1
61,113 + GFF2016 0.46 -0.7
61,114 - GFF2016 0.46 +0.0
61,114 - GFF2016 0.46 +0.1
61,114 - GFF2016 0.46 -0.4
61,114 - GFF2016 0.46 +1.2
61,114 - GFF2016 0.46 -0.6
61,114 - GFF2016 0.46 +0.4
61,114 - GFF2016 0.46 -0.7
61,114 - GFF2016 0.46 -0.7
61,164 + GFF2016 0.62 -0.1
61,164 + GFF2016 0.62 -2.2
61,164 + GFF2016 0.62 +0.1
61,164 + GFF2016 0.62 +1.2
61,164 + GFF2016 0.62 -0.5
61,164 + GFF2016 0.62 -1.1
61,164 + GFF2016 0.62 +0.1
61,165 - GFF2016 0.63 -0.0
61,165 - GFF2016 0.63 -0.3
61,165 - GFF2016 0.63 +0.4
61,165 - GFF2016 0.63 -0.3
61,165 - GFF2016 0.63 -0.1
61,165 - GFF2016 0.63 +0.8
61,221 + GFF2016 0.80 -1.0
61,221 + GFF2016 0.80 +0.6
61,221 + GFF2016 0.80 -1.0
61,222 - GFF2016 0.81 +0.2
61,224 + GFF2016 0.81 -0.2
61,224 + GFF2016 0.81 +0.3
61,224 + GFF2016 0.81 +1.3
61,224 + GFF2016 0.81 -0.8
61,225 - GFF2016 0.82 -0.0
61,225 - GFF2016 0.82 +0.8
61,225 - GFF2016 0.82 +0.8
61,225 - GFF2016 0.82 +0.2
61,225 - GFF2016 0.82 +0.4
61,225 - GFF2016 0.82 -0.2
61,225 - GFF2016 0.82 +1.1
61,254 + -0.2
61,255 - +0.0
61,260 + +0.1
61,260 + -0.2
61,260 + -1.9
61,260 + -0.8
61,260 + +0.6
61,260 + -0.1
61,261 - -0.5
61,261 - -0.6
61,261 - -0.8
61,261 - -0.8
61,261 - +0.4
61,261 - -0.9
61,261 - -0.2
61,261 - -0.3
61,261 - +1.3
61,261 - +0.1
61,261 - +0.3
61,261 - +0.2
61,316 + +1.3
61,316 + +0.2
61,317 - -0.2
61,317 - -0.2
61,317 - +1.1
61,317 - -0.6
61,317 - -0.4
61,317 - -0.1
61,351 - -0.9
61,351 - -2.7
61,379 + +1.8
61,649 + GFF2017 0.31 +0.0
61,649 + GFF2017 0.31 -0.2
61,649 + GFF2017 0.31 +0.3
61,650 - GFF2017 0.31 +0.7
61,650 - GFF2017 0.31 +0.9
61,650 - GFF2017 0.31 -0.6
61,650 - GFF2017 0.31 +0.6
61,695 - GFF2017 0.36 +0.2
61,695 - GFF2017 0.36 -0.2
61,727 + GFF2017 0.39 -0.0
62,007 - GFF2017 0.70 -0.3
62,007 - GFF2017 0.70 +2.3
62,007 - GFF2017 0.70 -0.4
62,528 + GFF2018 0.20 -0.1
62,528 + GFF2018 0.20 -1.7
62,528 + GFF2018 0.20 -0.1
62,528 + GFF2018 0.20 -0.3
62,528 + GFF2018 0.20 -0.9
62,528 + GFF2018 0.20 -0.1
62,528 + GFF2018 0.20 -1.2
62,528 + GFF2018 0.20 -0.4
62,528 + GFF2018 0.20 -0.1
62,528 + GFF2018 0.20 -0.1
62,528 + GFF2018 0.20 -0.9
62,528 + GFF2018 0.20 -0.0
62,528 + GFF2018 0.20 -1.4
62,528 + GFF2018 0.20 +0.2
62,528 + GFF2018 0.20 -0.7
62,528 + GFF2018 0.20 -0.3
62,528 + GFF2018 0.20 -0.3
62,529 - GFF2018 0.20 +0.6
62,529 - GFF2018 0.20 -0.3
62,529 - GFF2018 0.20 -0.9
62,529 - GFF2018 0.20 +0.2
62,529 - GFF2018 0.20 -0.5
62,529 - GFF2018 0.20 +0.2
62,529 - GFF2018 0.20 -0.3
62,529 - GFF2018 0.20 -0.2
62,529 - GFF2018 0.20 -1.0
62,529 - GFF2018 0.20 -0.1
62,529 - GFF2018 0.20 -1.0
62,529 - GFF2018 0.20 -0.1
62,529 - GFF2018 0.20 -0.2
62,529 - GFF2018 0.20 +0.3
62,534 + GFF2018 0.21 -0.6
62,534 + GFF2018 0.21 +0.1
62,534 + GFF2018 0.21 -1.0
62,534 + GFF2018 0.21 -1.0
62,534 + GFF2018 0.21 -1.0
62,534 + GFF2018 0.21 -0.6
62,534 + GFF2018 0.21 +0.4
62,535 - GFF2018 0.21 +0.3
62,535 - GFF2018 0.21 -0.3
62,535 - GFF2018 0.21 +0.1
62,535 - GFF2018 0.21 +0.3
62,558 + GFF2018 0.24 -0.4
62,558 + GFF2018 0.24 -0.0
62,559 - GFF2018 0.24 +0.2
62,559 - GFF2018 0.24 +0.9
62,559 - GFF2018 0.24 -0.8
62,562 - GFF2018 0.25 +0.4
62,562 - GFF2018 0.25 +1.6
62,711 + GFF2018 0.44 -1.3
62,711 + GFF2018 0.44 -0.8
62,711 + GFF2018 0.44 -0.6
62,711 + GFF2018 0.44 -0.5
62,711 + GFF2018 0.44 +0.3
62,711 + GFF2018 0.44 -2.2
62,712 - GFF2018 0.45 -1.2
62,747 + GFF2018 0.49 -0.7
62,747 + GFF2018 0.49 +0.7
63,011 + GFF2018 0.84 -0.6
63,011 + GFF2018 0.84 -1.4
63,011 + GFF2018 0.84 -0.2
63,011 + GFF2018 0.84 -1.9
63,012 - GFF2018 0.85 -0.6
63,012 - GFF2018 0.85 +0.2
63,012 - GFF2018 0.85 +0.1
63,012 - GFF2018 0.85 -0.4
63,113 + +0.2
63,151 + -0.5
63,152 - -0.5

Or see this region's nucleotide sequence