Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2014 and GFF2015 are separated by 44 nucleotides GFF2015 and GFF2016 are separated by 6 nucleotides GFF2016 and GFF2017 are separated by 81 nucleotides
GFF2014 - hypothetical protein, at 59,331 to 60,296
GFF2014
GFF2015 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 60,341 to 60,961
GFF2015
GFF2016 - hypothetical protein, at 60,968 to 61,282
GFF2016
GFF2017 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 61,364 to 62,284
GFF2017
Position (kb) 60
61
62 Strain fitness (log2 ratio) -2
-1
0
1
2 at 60.126 kb on + strand, within GFF2014 at 60.127 kb on - strand, within GFF2014 at 60.303 kb on + strand at 60.304 kb on - strand at 60.304 kb on - strand at 60.362 kb on + strand at 60.362 kb on + strand at 60.389 kb on + strand at 60.390 kb on - strand at 60.390 kb on - strand at 60.390 kb on - strand at 60.569 kb on + strand, within GFF2015 at 60.569 kb on + strand, within GFF2015 at 60.569 kb on + strand, within GFF2015 at 60.570 kb on - strand, within GFF2015 at 60.570 kb on - strand, within GFF2015 at 60.570 kb on - strand, within GFF2015 at 60.902 kb on + strand at 60.902 kb on + strand at 60.902 kb on + strand at 61.017 kb on + strand, within GFF2016 at 61.017 kb on + strand, within GFF2016 at 61.017 kb on + strand, within GFF2016 at 61.017 kb on + strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.221 kb on + strand, within GFF2016 at 61.221 kb on + strand, within GFF2016 at 61.221 kb on + strand, within GFF2016 at 61.222 kb on - strand, within GFF2016 at 61.224 kb on + strand, within GFF2016 at 61.224 kb on + strand, within GFF2016 at 61.224 kb on + strand, within GFF2016 at 61.224 kb on + strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.254 kb on + strand at 61.255 kb on - strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.316 kb on + strand at 61.316 kb on + strand at 61.317 kb on - strand at 61.317 kb on - strand at 61.317 kb on - strand at 61.317 kb on - strand at 61.317 kb on - strand at 61.317 kb on - strand at 61.351 kb on - strand at 61.351 kb on - strand at 61.379 kb on + strand at 61.649 kb on + strand, within GFF2017 at 61.649 kb on + strand, within GFF2017 at 61.649 kb on + strand, within GFF2017 at 61.650 kb on - strand, within GFF2017 at 61.650 kb on - strand, within GFF2017 at 61.650 kb on - strand, within GFF2017 at 61.650 kb on - strand, within GFF2017 at 61.695 kb on - strand, within GFF2017 at 61.695 kb on - strand, within GFF2017 at 61.727 kb on + strand, within GFF2017 at 62.007 kb on - strand, within GFF2017 at 62.007 kb on - strand, within GFF2017 at 62.007 kb on - strand, within GFF2017
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 60,126 + GFF2014 0.82 +0.6 60,127 - GFF2014 0.82 -0.7 60,303 + +0.2 60,304 - +0.2 60,304 - +0.3 60,362 + -0.2 60,362 + +1.2 60,389 + -1.6 60,390 - -0.1 60,390 - -0.3 60,390 - -0.4 60,569 + GFF2015 0.37 -0.6 60,569 + GFF2015 0.37 +0.4 60,569 + GFF2015 0.37 -0.2 60,570 - GFF2015 0.37 +0.3 60,570 - GFF2015 0.37 -0.5 60,570 - GFF2015 0.37 -0.5 60,902 + -0.5 60,902 + -0.7 60,902 + -0.7 61,017 + GFF2016 0.16 +0.5 61,017 + GFF2016 0.16 -0.8 61,017 + GFF2016 0.16 +1.8 61,017 + GFF2016 0.16 -0.6 61,018 - GFF2016 0.16 -0.8 61,018 - GFF2016 0.16 -0.8 61,018 - GFF2016 0.16 +0.0 61,018 - GFF2016 0.16 +0.2 61,018 - GFF2016 0.16 -0.5 61,113 + GFF2016 0.46 +0.1 61,113 + GFF2016 0.46 -0.4 61,113 + GFF2016 0.46 +0.2 61,113 + GFF2016 0.46 -0.1 61,113 + GFF2016 0.46 -0.3 61,113 + GFF2016 0.46 -0.5 61,113 + GFF2016 0.46 -0.3 61,113 + GFF2016 0.46 -0.1 61,113 + GFF2016 0.46 -0.7 61,114 - GFF2016 0.46 +0.0 61,114 - GFF2016 0.46 +0.1 61,114 - GFF2016 0.46 -0.4 61,114 - GFF2016 0.46 +1.2 61,114 - GFF2016 0.46 -0.6 61,114 - GFF2016 0.46 +0.4 61,114 - GFF2016 0.46 -0.7 61,114 - GFF2016 0.46 -0.7 61,164 + GFF2016 0.62 -0.1 61,164 + GFF2016 0.62 -2.2 61,164 + GFF2016 0.62 +0.1 61,164 + GFF2016 0.62 +1.2 61,164 + GFF2016 0.62 -0.5 61,164 + GFF2016 0.62 -1.1 61,164 + GFF2016 0.62 +0.1 61,165 - GFF2016 0.63 -0.0 61,165 - GFF2016 0.63 -0.3 61,165 - GFF2016 0.63 +0.4 61,165 - GFF2016 0.63 -0.3 61,165 - GFF2016 0.63 -0.1 61,165 - GFF2016 0.63 +0.8 61,221 + GFF2016 0.80 -1.0 61,221 + GFF2016 0.80 +0.6 61,221 + GFF2016 0.80 -1.0 61,222 - GFF2016 0.81 +0.2 61,224 + GFF2016 0.81 -0.2 61,224 + GFF2016 0.81 +0.3 61,224 + GFF2016 0.81 +1.3 61,224 + GFF2016 0.81 -0.8 61,225 - GFF2016 0.82 -0.0 61,225 - GFF2016 0.82 +0.8 61,225 - GFF2016 0.82 +0.8 61,225 - GFF2016 0.82 +0.2 61,225 - GFF2016 0.82 +0.4 61,225 - GFF2016 0.82 -0.2 61,225 - GFF2016 0.82 +1.1 61,254 + -0.2 61,255 - +0.0 61,260 + +0.1 61,260 + -0.2 61,260 + -1.9 61,260 + -0.8 61,260 + +0.6 61,260 + -0.1 61,261 - -0.5 61,261 - -0.6 61,261 - -0.8 61,261 - -0.8 61,261 - +0.4 61,261 - -0.9 61,261 - -0.2 61,261 - -0.3 61,261 - +1.3 61,261 - +0.1 61,261 - +0.3 61,261 - +0.2 61,316 + +1.3 61,316 + +0.2 61,317 - -0.2 61,317 - -0.2 61,317 - +1.1 61,317 - -0.6 61,317 - -0.4 61,317 - -0.1 61,351 - -0.9 61,351 - -2.7 61,379 + +1.8 61,649 + GFF2017 0.31 +0.0 61,649 + GFF2017 0.31 -0.2 61,649 + GFF2017 0.31 +0.3 61,650 - GFF2017 0.31 +0.7 61,650 - GFF2017 0.31 +0.9 61,650 - GFF2017 0.31 -0.6 61,650 - GFF2017 0.31 +0.6 61,695 - GFF2017 0.36 +0.2 61,695 - GFF2017 0.36 -0.2 61,727 + GFF2017 0.39 -0.0 62,007 - GFF2017 0.70 -0.3 62,007 - GFF2017 0.70 +2.3 62,007 - GFF2017 0.70 -0.4
Or see this region's nucleotide sequence