Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF189 and GFF190 are separated by 43 nucleotides GFF190 and GFF191 overlap by 1 nucleotides GFF191 and GFF192 are separated by 117 nucleotides GFF192 and GFF193 overlap by 4 nucleotides
GFF189 - Negative transcriptional regulator, at 219,422 to 220,066
GFF189
GFF190 - Ankyrin, at 220,110 to 220,808
GFF190
GFF191 - (Y14336) putative extracellular protein containing predicted 35aa signal peptide, at 220,808 to 221,308
GFF191
GFF192 - Nitrogen regulation protein NR(I), GlnG (=NtrC), at 221,426 to 222,067
GFF192
GFF193 - Two-component sensor PilS, at 222,064 to 223,569
GFF193
Position (kb)
220
221
222 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.864 kb on + strand, within GFF189 at 219.865 kb on - strand, within GFF189 at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.111 kb on + strand at 220.112 kb on - strand at 220.112 kb on - strand at 220.438 kb on - strand, within GFF190 at 220.501 kb on + strand, within GFF190 at 220.502 kb on - strand, within GFF190 at 220.699 kb on + strand, within GFF190 at 220.699 kb on + strand, within GFF190 at 220.699 kb on + strand, within GFF190 at 220.700 kb on - strand, within GFF190 at 220.700 kb on - strand, within GFF190 at 220.700 kb on - strand, within GFF190 at 220.700 kb on - strand, within GFF190 at 220.700 kb on - strand, within GFF190 at 220.700 kb on - strand, within GFF190 at 220.959 kb on + strand, within GFF191 at 220.959 kb on + strand, within GFF191 at 220.959 kb on + strand, within GFF191 at 220.959 kb on + strand, within GFF191 at 220.959 kb on + strand, within GFF191 at 220.959 kb on + strand, within GFF191 at 220.959 kb on + strand, within GFF191 at 220.959 kb on + strand, within GFF191 at 220.959 kb on + strand, within GFF191 at 220.959 kb on + strand, within GFF191 at 220.960 kb on - strand, within GFF191 at 220.960 kb on - strand, within GFF191 at 220.960 kb on - strand, within GFF191 at 221.145 kb on + strand, within GFF191 at 221.145 kb on + strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.370 kb on + strand at 221.371 kb on - strand at 221.399 kb on + strand at 221.400 kb on - strand at 221.421 kb on + strand at 221.422 kb on - strand at 221.427 kb on + strand at 221.428 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 219,813 + GFF189 0.61 -1.8 219,813 + GFF189 0.61 +0.7 219,813 + GFF189 0.61 -0.7 219,813 + GFF189 0.61 -0.4 219,813 + GFF189 0.61 -0.5 219,813 + GFF189 0.61 +1.3 219,813 + GFF189 0.61 -0.8 219,813 + GFF189 0.61 -1.2 219,813 + GFF189 0.61 -1.5 219,813 + GFF189 0.61 +0.3 219,813 + GFF189 0.61 -0.4 219,813 + GFF189 0.61 -1.8 219,813 + GFF189 0.61 +1.3 219,813 + GFF189 0.61 -0.3 219,813 + GFF189 0.61 -0.5 219,813 + GFF189 0.61 +1.3 219,813 + GFF189 0.61 +0.7 219,813 + GFF189 0.61 +1.6 219,813 + GFF189 0.61 -0.1 219,813 + GFF189 0.61 +1.3 219,814 - GFF189 0.61 -1.0 219,814 - GFF189 0.61 +0.2 219,814 - GFF189 0.61 -1.2 219,814 - GFF189 0.61 +1.3 219,814 - GFF189 0.61 +0.1 219,814 - GFF189 0.61 -0.3 219,814 - GFF189 0.61 +1.0 219,814 - GFF189 0.61 -0.4 219,814 - GFF189 0.61 -0.9 219,814 - GFF189 0.61 +0.3 219,814 - GFF189 0.61 -0.6 219,814 - GFF189 0.61 -1.9 219,814 - GFF189 0.61 -0.1 219,814 - GFF189 0.61 -0.3 219,814 - GFF189 0.61 -1.5 219,814 - GFF189 0.61 -0.1 219,814 - GFF189 0.61 -0.1 219,814 - GFF189 0.61 -0.2 219,814 - GFF189 0.61 -0.3 219,814 - GFF189 0.61 -0.5 219,814 - GFF189 0.61 -0.3 219,864 + GFF189 0.69 -0.6 219,865 - GFF189 0.69 +0.3 220,062 + -1.3 220,062 + -0.2 220,062 + -0.9 220,062 + +0.2 220,062 + -0.1 220,062 + -0.2 220,062 + +1.1 220,062 + -0.5 220,062 + -1.9 220,062 + -0.9 220,062 + -0.4 220,062 + -0.5 220,062 + -0.3 220,062 + -0.1 220,062 + -0.2 220,062 + +0.3 220,062 + +0.8 220,062 + -0.7 220,062 + -1.9 220,062 + -0.7 220,062 + +0.8 220,062 + -0.3 220,062 + -0.4 220,062 + -0.5 220,062 + -1.0 220,063 - -0.6 220,063 - +0.3 220,063 - +0.7 220,063 - +1.7 220,063 - +0.3 220,063 - -0.6 220,063 - -0.1 220,063 - -1.7 220,063 - -0.0 220,063 - -0.4 220,063 - -0.5 220,111 + -1.3 220,112 - +1.0 220,112 - +0.3 220,438 - GFF190 0.47 -1.1 220,501 + GFF190 0.56 -1.3 220,502 - GFF190 0.56 +0.7 220,699 + GFF190 0.84 -0.2 220,699 + GFF190 0.84 -0.5 220,699 + GFF190 0.84 -0.2 220,700 - GFF190 0.84 +0.0 220,700 - GFF190 0.84 -0.1 220,700 - GFF190 0.84 -0.2 220,700 - GFF190 0.84 -0.7 220,700 - GFF190 0.84 -0.6 220,700 - GFF190 0.84 -0.1 220,959 + GFF191 0.30 +0.7 220,959 + GFF191 0.30 +0.4 220,959 + GFF191 0.30 +0.2 220,959 + GFF191 0.30 -0.1 220,959 + GFF191 0.30 +0.2 220,959 + GFF191 0.30 -0.7 220,959 + GFF191 0.30 +0.5 220,959 + GFF191 0.30 -1.5 220,959 + GFF191 0.30 -0.5 220,959 + GFF191 0.30 -0.3 220,960 - GFF191 0.30 -1.0 220,960 - GFF191 0.30 +1.3 220,960 - GFF191 0.30 +0.7 221,145 + GFF191 0.67 -0.0 221,145 + GFF191 0.67 +0.3 221,146 - GFF191 0.67 +0.1 221,146 - GFF191 0.67 -0.1 221,146 - GFF191 0.67 +0.7 221,146 - GFF191 0.67 -0.5 221,370 + -0.1 221,371 - +0.4 221,399 + +2.2 221,400 - -0.0 221,421 + -0.4 221,422 - -0.3 221,427 + +0.5 221,428 - -2.2
Or see this region's nucleotide sequence