Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1875 and GFF1874 overlap by 2 nucleotides GFF1874 and GFF1876 are separated by 12 nucleotides GFF1876 and GFF1877 are separated by 9 nucleotides GFF1877 and GFF1878 are separated by 33 nucleotides
GFF1875 - no description, at 548,492 to 548,626
GFF1875
GFF1874 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 548,625 to 549,581
GFF1874
GFF1876 - Dihydrodipicolinate synthase family, at 549,594 to 550,487
GFF1876
GFF1877 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 550,497 to 551,279
GFF1877
GFF1878 - Bll6165 protein, at 551,313 to 551,723
GFF1878
Position (kb) 549
550
551 Strain fitness (log2 ratio) -2
-1
0
1
2 at 549.249 kb on + strand, within GFF1874 at 549.250 kb on - strand, within GFF1874 at 549.390 kb on + strand, within GFF1874 at 549.390 kb on + strand, within GFF1874 at 549.402 kb on + strand, within GFF1874 at 549.402 kb on + strand, within GFF1874 at 549.403 kb on - strand, within GFF1874 at 549.615 kb on + strand at 549.615 kb on + strand at 549.615 kb on + strand at 549.615 kb on + strand at 549.615 kb on + strand at 549.616 kb on - strand at 549.616 kb on - strand at 549.678 kb on + strand at 549.678 kb on + strand at 549.678 kb on + strand at 549.678 kb on + strand at 549.918 kb on + strand, within GFF1876 at 549.919 kb on - strand, within GFF1876 at 549.919 kb on - strand, within GFF1876 at 549.954 kb on + strand, within GFF1876 at 549.954 kb on + strand, within GFF1876 at 549.955 kb on - strand, within GFF1876 at 549.955 kb on - strand, within GFF1876 at 549.996 kb on + strand, within GFF1876 at 549.996 kb on + strand, within GFF1876 at 549.996 kb on + strand, within GFF1876 at 549.996 kb on + strand, within GFF1876 at 549.997 kb on - strand, within GFF1876 at 549.997 kb on - strand, within GFF1876 at 550.074 kb on + strand, within GFF1876 at 550.075 kb on - strand, within GFF1876 at 550.075 kb on - strand, within GFF1876 at 550.075 kb on - strand, within GFF1876 at 550.176 kb on + strand, within GFF1876 at 550.176 kb on + strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.245 kb on + strand, within GFF1876 at 550.246 kb on - strand, within GFF1876 at 550.246 kb on - strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.490 kb on + strand at 550.490 kb on + strand at 550.490 kb on + strand at 550.491 kb on - strand at 550.951 kb on - strand, within GFF1877 at 551.287 kb on + strand at 551.287 kb on + strand at 551.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 549,249 + GFF1874 0.65 +0.3 549,250 - GFF1874 0.65 +1.1 549,390 + GFF1874 0.80 +0.3 549,390 + GFF1874 0.80 +0.1 549,402 + GFF1874 0.81 -0.8 549,402 + GFF1874 0.81 -0.5 549,403 - GFF1874 0.81 -0.1 549,615 + -1.2 549,615 + -0.8 549,615 + -0.7 549,615 + +0.1 549,615 + +0.2 549,616 - +0.8 549,616 - +1.3 549,678 + -0.1 549,678 + -0.6 549,678 + -0.4 549,678 + -0.5 549,918 + GFF1876 0.36 -0.3 549,919 - GFF1876 0.36 +0.3 549,919 - GFF1876 0.36 +0.1 549,954 + GFF1876 0.40 -0.1 549,954 + GFF1876 0.40 +0.1 549,955 - GFF1876 0.40 -0.3 549,955 - GFF1876 0.40 +0.1 549,996 + GFF1876 0.45 +0.7 549,996 + GFF1876 0.45 -0.5 549,996 + GFF1876 0.45 -0.1 549,996 + GFF1876 0.45 -1.0 549,997 - GFF1876 0.45 -0.3 549,997 - GFF1876 0.45 -0.1 550,074 + GFF1876 0.54 -0.0 550,075 - GFF1876 0.54 +0.8 550,075 - GFF1876 0.54 +1.3 550,075 - GFF1876 0.54 +0.1 550,176 + GFF1876 0.65 +0.4 550,176 + GFF1876 0.65 +0.7 550,177 - GFF1876 0.65 -0.5 550,177 - GFF1876 0.65 -0.9 550,177 - GFF1876 0.65 -0.1 550,177 - GFF1876 0.65 +0.6 550,177 - GFF1876 0.65 +0.5 550,245 + GFF1876 0.73 +0.9 550,246 - GFF1876 0.73 +0.3 550,246 - GFF1876 0.73 -0.4 550,335 + GFF1876 0.83 +0.4 550,335 + GFF1876 0.83 -0.2 550,335 + GFF1876 0.83 +0.2 550,335 + GFF1876 0.83 -0.6 550,336 - GFF1876 0.83 +0.7 550,336 - GFF1876 0.83 +0.0 550,336 - GFF1876 0.83 -0.5 550,336 - GFF1876 0.83 -0.8 550,490 + -1.3 550,490 + -0.6 550,490 + -0.5 550,491 - -2.2 550,951 - GFF1877 0.58 +0.1 551,287 + -0.2 551,287 + +2.2 551,295 - -2.2
Or see this region's nucleotide sequence