Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1858 and GFF1859 overlap by 4 nucleotides GFF1859 and GFF1860 are separated by 12 nucleotides GFF1860 and GFF1861 are separated by 172 nucleotides
GFF1858 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 533,115 to 533,879
GFF1858
GFF1859 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 533,876 to 534,547
GFF1859
GFF1860 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 534,560 to 535,327
GFF1860
GFF1861 - Transcriptional regulator, IclR family, at 535,500 to 536,324
GFF1861
Position (kb)
534
535
536 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 533.722 kb on + strand, within GFF1858 at 533.928 kb on + strand at 533.928 kb on + strand at 533.928 kb on + strand at 533.928 kb on + strand at 533.929 kb on - strand at 533.929 kb on - strand at 533.929 kb on - strand at 533.955 kb on + strand, within GFF1859 at 533.955 kb on + strand, within GFF1859 at 533.955 kb on + strand, within GFF1859 at 533.956 kb on - strand, within GFF1859 at 533.956 kb on - strand, within GFF1859 at 533.956 kb on - strand, within GFF1859 at 533.956 kb on - strand, within GFF1859 at 533.976 kb on + strand, within GFF1859 at 533.976 kb on + strand, within GFF1859 at 533.977 kb on - strand, within GFF1859 at 534.097 kb on - strand, within GFF1859 at 534.097 kb on - strand, within GFF1859 at 534.132 kb on - strand, within GFF1859 at 534.132 kb on - strand, within GFF1859 at 534.318 kb on + strand, within GFF1859 at 534.318 kb on + strand, within GFF1859 at 534.318 kb on + strand, within GFF1859 at 534.318 kb on + strand, within GFF1859 at 534.318 kb on + strand, within GFF1859 at 534.318 kb on + strand, within GFF1859 at 534.319 kb on - strand, within GFF1859 at 534.319 kb on - strand, within GFF1859 at 534.319 kb on - strand, within GFF1859 at 534.319 kb on - strand, within GFF1859 at 534.319 kb on - strand, within GFF1859 at 534.319 kb on - strand, within GFF1859 at 534.324 kb on + strand, within GFF1859 at 534.324 kb on + strand, within GFF1859 at 534.324 kb on + strand, within GFF1859 at 534.324 kb on + strand, within GFF1859 at 534.324 kb on + strand, within GFF1859 at 534.324 kb on + strand, within GFF1859 at 534.324 kb on + strand, within GFF1859 at 534.324 kb on + strand, within GFF1859 at 534.325 kb on - strand, within GFF1859 at 534.325 kb on - strand, within GFF1859 at 534.325 kb on - strand, within GFF1859 at 534.325 kb on - strand, within GFF1859 at 534.325 kb on - strand, within GFF1859 at 534.325 kb on - strand, within GFF1859 at 534.325 kb on - strand, within GFF1859 at 534.699 kb on + strand, within GFF1860 at 534.699 kb on + strand, within GFF1860 at 534.700 kb on - strand, within GFF1860 at 534.700 kb on - strand, within GFF1860 at 534.700 kb on - strand, within GFF1860 at 534.700 kb on - strand, within GFF1860 at 534.700 kb on - strand, within GFF1860 at 534.700 kb on - strand, within GFF1860 at 534.700 kb on - strand, within GFF1860 at 534.700 kb on - strand, within GFF1860 at 534.700 kb on - strand, within GFF1860 at 534.747 kb on + strand, within GFF1860 at 534.747 kb on + strand, within GFF1860 at 534.748 kb on - strand, within GFF1860 at 534.748 kb on - strand, within GFF1860 at 534.828 kb on + strand, within GFF1860 at 534.828 kb on + strand, within GFF1860 at 534.828 kb on + strand, within GFF1860 at 534.828 kb on + strand, within GFF1860 at 534.828 kb on + strand, within GFF1860 at 534.829 kb on - strand, within GFF1860 at 534.829 kb on - strand, within GFF1860 at 534.933 kb on + strand, within GFF1860 at 534.933 kb on + strand, within GFF1860 at 534.933 kb on + strand, within GFF1860 at 534.933 kb on + strand, within GFF1860 at 534.933 kb on + strand, within GFF1860 at 534.934 kb on - strand, within GFF1860 at 534.975 kb on + strand, within GFF1860 at 534.975 kb on + strand, within GFF1860 at 534.976 kb on - strand, within GFF1860 at 534.981 kb on + strand, within GFF1860 at 534.982 kb on - strand, within GFF1860 at 534.982 kb on - strand, within GFF1860 at 535.230 kb on + strand, within GFF1860 at 535.230 kb on + strand, within GFF1860 at 535.230 kb on + strand, within GFF1860 at 535.231 kb on - strand, within GFF1860 at 535.231 kb on - strand, within GFF1860 at 535.231 kb on - strand, within GFF1860 at 535.507 kb on + strand at 535.507 kb on + strand at 535.508 kb on - strand at 535.508 kb on - strand at 535.510 kb on - strand at 535.716 kb on + strand, within GFF1861 at 535.747 kb on - strand, within GFF1861 at 535.785 kb on + strand, within GFF1861 at 535.785 kb on + strand, within GFF1861 at 535.786 kb on - strand, within GFF1861 at 535.786 kb on - strand, within GFF1861 at 535.786 kb on - strand, within GFF1861 at 535.896 kb on + strand, within GFF1861 at 535.896 kb on + strand, within GFF1861 at 535.896 kb on + strand, within GFF1861 at 535.896 kb on + strand, within GFF1861 at 535.897 kb on - strand, within GFF1861 at 535.897 kb on - strand, within GFF1861 at 535.897 kb on - strand, within GFF1861 at 535.897 kb on - strand, within GFF1861 at 535.897 kb on - strand, within GFF1861 at 535.965 kb on + strand, within GFF1861 at 535.965 kb on + strand, within GFF1861 at 535.965 kb on + strand, within GFF1861 at 535.965 kb on + strand, within GFF1861 at 535.965 kb on + strand, within GFF1861 at 535.965 kb on + strand, within GFF1861 at 535.965 kb on + strand, within GFF1861 at 535.965 kb on + strand, within GFF1861 at 535.965 kb on + strand, within GFF1861 at 535.966 kb on - strand, within GFF1861 at 535.966 kb on - strand, within GFF1861 at 535.966 kb on - strand, within GFF1861 at 535.966 kb on - strand, within GFF1861 at 535.966 kb on - strand, within GFF1861 at 535.966 kb on - strand, within GFF1861 at 535.966 kb on - strand, within GFF1861 at 535.966 kb on - strand, within GFF1861
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 533,722 + GFF1858 0.79 +0.5 533,928 + +0.2 533,928 + -0.5 533,928 + -0.7 533,928 + -0.9 533,929 - +1.1 533,929 - +1.7 533,929 - +0.5 533,955 + GFF1859 0.12 -2.0 533,955 + GFF1859 0.12 -0.3 533,955 + GFF1859 0.12 +0.4 533,956 - GFF1859 0.12 -0.0 533,956 - GFF1859 0.12 -0.1 533,956 - GFF1859 0.12 -1.3 533,956 - GFF1859 0.12 -0.5 533,976 + GFF1859 0.15 -0.3 533,976 + GFF1859 0.15 -0.3 533,977 - GFF1859 0.15 +0.1 534,097 - GFF1859 0.33 -0.2 534,097 - GFF1859 0.33 -0.1 534,132 - GFF1859 0.38 -1.3 534,132 - GFF1859 0.38 -1.6 534,318 + GFF1859 0.66 +0.7 534,318 + GFF1859 0.66 -0.0 534,318 + GFF1859 0.66 +0.1 534,318 + GFF1859 0.66 -0.3 534,318 + GFF1859 0.66 -1.5 534,318 + GFF1859 0.66 +0.1 534,319 - GFF1859 0.66 -0.1 534,319 - GFF1859 0.66 +0.4 534,319 - GFF1859 0.66 -0.2 534,319 - GFF1859 0.66 +0.1 534,319 - GFF1859 0.66 +0.7 534,319 - GFF1859 0.66 -0.0 534,324 + GFF1859 0.67 -0.0 534,324 + GFF1859 0.67 +1.0 534,324 + GFF1859 0.67 -1.1 534,324 + GFF1859 0.67 -0.3 534,324 + GFF1859 0.67 +0.1 534,324 + GFF1859 0.67 -0.9 534,324 + GFF1859 0.67 +1.0 534,324 + GFF1859 0.67 -0.9 534,325 - GFF1859 0.67 -0.0 534,325 - GFF1859 0.67 -0.3 534,325 - GFF1859 0.67 -0.0 534,325 - GFF1859 0.67 -0.1 534,325 - GFF1859 0.67 -0.8 534,325 - GFF1859 0.67 +0.5 534,325 - GFF1859 0.67 -1.3 534,699 + GFF1860 0.18 +0.0 534,699 + GFF1860 0.18 -0.9 534,700 - GFF1860 0.18 -0.5 534,700 - GFF1860 0.18 -1.5 534,700 - GFF1860 0.18 -0.6 534,700 - GFF1860 0.18 -0.5 534,700 - GFF1860 0.18 -1.4 534,700 - GFF1860 0.18 -0.0 534,700 - GFF1860 0.18 +1.1 534,700 - GFF1860 0.18 -0.3 534,700 - GFF1860 0.18 -1.6 534,747 + GFF1860 0.24 +0.1 534,747 + GFF1860 0.24 -0.3 534,748 - GFF1860 0.24 +0.5 534,748 - GFF1860 0.24 +0.4 534,828 + GFF1860 0.35 -0.8 534,828 + GFF1860 0.35 -1.2 534,828 + GFF1860 0.35 -1.9 534,828 + GFF1860 0.35 -0.6 534,828 + GFF1860 0.35 -0.3 534,829 - GFF1860 0.35 +0.4 534,829 - GFF1860 0.35 -0.0 534,933 + GFF1860 0.49 +0.1 534,933 + GFF1860 0.49 -0.2 534,933 + GFF1860 0.49 -0.3 534,933 + GFF1860 0.49 -2.1 534,933 + GFF1860 0.49 -0.2 534,934 - GFF1860 0.49 -0.9 534,975 + GFF1860 0.54 -1.5 534,975 + GFF1860 0.54 +0.7 534,976 - GFF1860 0.54 -0.6 534,981 + GFF1860 0.55 -0.9 534,982 - GFF1860 0.55 -0.9 534,982 - GFF1860 0.55 -0.8 535,230 + GFF1860 0.87 -0.3 535,230 + GFF1860 0.87 +1.1 535,230 + GFF1860 0.87 -0.5 535,231 - GFF1860 0.87 -0.1 535,231 - GFF1860 0.87 -0.4 535,231 - GFF1860 0.87 -0.9 535,507 + +0.5 535,507 + +1.3 535,508 - +0.4 535,508 - +1.1 535,510 - +1.1 535,716 + GFF1861 0.26 +0.8 535,747 - GFF1861 0.30 -0.2 535,785 + GFF1861 0.35 +0.4 535,785 + GFF1861 0.35 +0.0 535,786 - GFF1861 0.35 +0.8 535,786 - GFF1861 0.35 +0.7 535,786 - GFF1861 0.35 +0.7 535,896 + GFF1861 0.48 +1.2 535,896 + GFF1861 0.48 +0.4 535,896 + GFF1861 0.48 +0.3 535,896 + GFF1861 0.48 +0.2 535,897 - GFF1861 0.48 +0.4 535,897 - GFF1861 0.48 +0.1 535,897 - GFF1861 0.48 +2.3 535,897 - GFF1861 0.48 -0.8 535,897 - GFF1861 0.48 +0.3 535,965 + GFF1861 0.56 +0.4 535,965 + GFF1861 0.56 -0.2 535,965 + GFF1861 0.56 +0.5 535,965 + GFF1861 0.56 -1.1 535,965 + GFF1861 0.56 +0.4 535,965 + GFF1861 0.56 -0.8 535,965 + GFF1861 0.56 +1.9 535,965 + GFF1861 0.56 +1.9 535,965 + GFF1861 0.56 +0.3 535,966 - GFF1861 0.56 +0.4 535,966 - GFF1861 0.56 +0.4 535,966 - GFF1861 0.56 +0.2 535,966 - GFF1861 0.56 -0.4 535,966 - GFF1861 0.56 +0.5 535,966 - GFF1861 0.56 +0.1 535,966 - GFF1861 0.56 +0.8 535,966 - GFF1861 0.56 +0.7
Or see this region's nucleotide sequence