Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1746 and GFF1747 are separated by 100 nucleotides GFF1747 and GFF1748 are separated by 90 nucleotides GFF1748 and GFF1749 are separated by 21 nucleotides
GFF1746 - BUG/TctC family periplasmic protein, at 406,563 to 407,561
GFF1746
GFF1747 - FIG00799528: hypothetical protein, at 407,662 to 408,759
GFF1747
GFF1748 - Outer membrane lipoprotein, at 408,850 to 409,434
GFF1748
GFF1749 - Cystathionine beta-lyase (EC 4.4.1.8), at 409,456 to 410,670
GFF1749
Position (kb) 407
408
409 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 406.851 kb on + strand, within GFF1746 at 406.852 kb on - strand, within GFF1746 at 406.863 kb on + strand, within GFF1746 at 406.863 kb on + strand, within GFF1746 at 406.899 kb on + strand, within GFF1746 at 406.899 kb on + strand, within GFF1746 at 406.900 kb on - strand, within GFF1746 at 406.900 kb on - strand, within GFF1746 at 406.900 kb on - strand, within GFF1746 at 406.900 kb on - strand, within GFF1746 at 406.900 kb on - strand, within GFF1746 at 406.915 kb on - strand, within GFF1746 at 407.139 kb on + strand, within GFF1746 at 407.139 kb on + strand, within GFF1746 at 407.140 kb on - strand, within GFF1746 at 407.140 kb on - strand, within GFF1746 at 407.217 kb on + strand, within GFF1746 at 407.394 kb on + strand, within GFF1746 at 407.394 kb on + strand, within GFF1746 at 407.394 kb on + strand, within GFF1746 at 407.487 kb on + strand at 407.487 kb on + strand at 407.559 kb on + strand at 407.559 kb on + strand at 407.560 kb on - strand at 407.582 kb on - strand at 407.740 kb on + strand at 407.740 kb on + strand at 407.740 kb on + strand at 407.740 kb on + strand at 407.740 kb on + strand at 407.741 kb on - strand at 407.741 kb on - strand at 407.741 kb on - strand at 408.095 kb on - strand, within GFF1747 at 408.095 kb on - strand, within GFF1747 at 408.095 kb on - strand, within GFF1747 at 408.158 kb on - strand, within GFF1747 at 408.197 kb on - strand, within GFF1747 at 408.270 kb on + strand, within GFF1747 at 408.271 kb on - strand, within GFF1747 at 408.409 kb on + strand, within GFF1747 at 408.409 kb on + strand, within GFF1747 at 408.481 kb on + strand, within GFF1747 at 408.481 kb on + strand, within GFF1747 at 408.481 kb on + strand, within GFF1747 at 408.481 kb on + strand, within GFF1747 at 408.482 kb on - strand, within GFF1747 at 408.482 kb on - strand, within GFF1747 at 408.526 kb on + strand, within GFF1747 at 408.526 kb on + strand, within GFF1747 at 408.526 kb on + strand, within GFF1747 at 408.527 kb on - strand, within GFF1747 at 408.527 kb on - strand, within GFF1747 at 408.653 kb on - strand at 408.806 kb on - strand at 409.003 kb on + strand, within GFF1748 at 409.003 kb on + strand, within GFF1748 at 409.003 kb on + strand, within GFF1748 at 409.063 kb on + strand, within GFF1748 at 409.222 kb on + strand, within GFF1748 at 409.222 kb on + strand, within GFF1748 at 409.222 kb on + strand, within GFF1748 at 409.223 kb on - strand, within GFF1748 at 409.223 kb on - strand, within GFF1748 at 409.223 kb on - strand, within GFF1748 at 409.249 kb on + strand, within GFF1748 at 409.249 kb on + strand, within GFF1748 at 409.249 kb on + strand, within GFF1748 at 409.250 kb on - strand, within GFF1748 at 409.250 kb on - strand, within GFF1748 at 409.286 kb on - strand, within GFF1748 at 409.286 kb on - strand, within GFF1748 at 409.330 kb on + strand, within GFF1748 at 409.330 kb on + strand, within GFF1748 at 409.331 kb on - strand, within GFF1748 at 409.432 kb on + strand at 409.433 kb on - strand at 409.622 kb on + strand, within GFF1749
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 406,851 + GFF1746 0.29 +1.2 406,852 - GFF1746 0.29 +0.1 406,863 + GFF1746 0.30 -0.4 406,863 + GFF1746 0.30 -0.1 406,899 + GFF1746 0.34 +0.3 406,899 + GFF1746 0.34 -0.2 406,900 - GFF1746 0.34 +0.6 406,900 - GFF1746 0.34 +0.5 406,900 - GFF1746 0.34 -1.8 406,900 - GFF1746 0.34 -0.5 406,900 - GFF1746 0.34 +0.1 406,915 - GFF1746 0.35 +1.5 407,139 + GFF1746 0.58 -0.0 407,139 + GFF1746 0.58 -0.2 407,140 - GFF1746 0.58 +0.2 407,140 - GFF1746 0.58 -0.0 407,217 + GFF1746 0.65 -2.5 407,394 + GFF1746 0.83 -0.6 407,394 + GFF1746 0.83 -1.8 407,394 + GFF1746 0.83 -0.4 407,487 + -0.4 407,487 + -1.1 407,559 + -0.3 407,559 + +0.4 407,560 - -0.9 407,582 - -1.2 407,740 + -0.3 407,740 + -0.6 407,740 + -0.1 407,740 + -0.5 407,740 + +0.7 407,741 - -0.5 407,741 - -0.7 407,741 - +0.3 408,095 - GFF1747 0.39 -0.4 408,095 - GFF1747 0.39 -0.3 408,095 - GFF1747 0.39 -0.1 408,158 - GFF1747 0.45 -0.4 408,197 - GFF1747 0.49 -0.5 408,270 + GFF1747 0.55 +1.0 408,271 - GFF1747 0.55 -0.2 408,409 + GFF1747 0.68 -1.2 408,409 + GFF1747 0.68 +3.5 408,481 + GFF1747 0.75 -1.2 408,481 + GFF1747 0.75 +0.6 408,481 + GFF1747 0.75 +0.7 408,481 + GFF1747 0.75 -0.2 408,482 - GFF1747 0.75 -0.0 408,482 - GFF1747 0.75 -2.6 408,526 + GFF1747 0.79 +0.3 408,526 + GFF1747 0.79 +0.2 408,526 + GFF1747 0.79 +1.4 408,527 - GFF1747 0.79 -0.2 408,527 - GFF1747 0.79 +2.2 408,653 - -0.4 408,806 - -1.2 409,003 + GFF1748 0.26 -0.4 409,003 + GFF1748 0.26 -1.5 409,003 + GFF1748 0.26 +1.0 409,063 + GFF1748 0.36 -0.3 409,222 + GFF1748 0.64 -0.9 409,222 + GFF1748 0.64 -0.6 409,222 + GFF1748 0.64 -0.4 409,223 - GFF1748 0.64 -0.3 409,223 - GFF1748 0.64 -1.9 409,223 - GFF1748 0.64 -2.2 409,249 + GFF1748 0.68 -0.8 409,249 + GFF1748 0.68 -0.3 409,249 + GFF1748 0.68 -0.1 409,250 - GFF1748 0.68 +0.1 409,250 - GFF1748 0.68 -0.8 409,286 - GFF1748 0.75 +3.3 409,286 - GFF1748 0.75 -0.7 409,330 + GFF1748 0.82 +0.8 409,330 + GFF1748 0.82 -1.3 409,331 - GFF1748 0.82 -0.8 409,432 + -1.1 409,433 - -0.8 409,622 + GFF1749 0.14 -0.2
Or see this region's nucleotide sequence