Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF142 and GFF143 overlap by 18 nucleotides GFF143 and GFF144 overlap by 4 nucleotides GFF144 and GFF145 overlap by 17 nucleotides
GFF142 - Oxidoreductase, at 170,605 to 171,657
GFF142
GFF143 - Dethiobiotin synthase BioD (EC 6.3.3.3), at 171,640 to 172,386
GFF143
GFF144 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 172,383 to 173,621
GFF144
GFF145 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 173,605 to 174,984
GFF145
Position (kb) 172
173
174 Strain fitness (log2 ratio) -2
-1
0
1
2 at 171.583 kb on + strand at 172.160 kb on + strand, within GFF143 at 172.160 kb on + strand, within GFF143 at 172.161 kb on - strand, within GFF143 at 172.798 kb on + strand, within GFF144 at 172.798 kb on + strand, within GFF144 at 172.798 kb on + strand, within GFF144 at 172.799 kb on - strand, within GFF144 at 172.822 kb on + strand, within GFF144 at 172.822 kb on + strand, within GFF144 at 173.037 kb on + strand, within GFF144 at 173.037 kb on + strand, within GFF144 at 173.038 kb on - strand, within GFF144 at 173.038 kb on - strand, within GFF144 at 173.038 kb on - strand, within GFF144 at 173.140 kb on + strand, within GFF144 at 173.262 kb on - strand, within GFF144 at 173.263 kb on + strand, within GFF144 at 173.263 kb on + strand, within GFF144 at 173.264 kb on - strand, within GFF144 at 173.264 kb on - strand, within GFF144 at 173.264 kb on - strand, within GFF144 at 173.264 kb on - strand, within GFF144 at 173.264 kb on - strand, within GFF144 at 173.455 kb on + strand, within GFF144 at 173.455 kb on + strand, within GFF144 at 173.717 kb on + strand at 173.717 kb on + strand at 173.717 kb on + strand at 173.718 kb on - strand at 173.732 kb on + strand at 173.732 kb on + strand at 173.732 kb on + strand at 173.733 kb on - strand at 173.733 kb on - strand at 173.733 kb on - strand at 173.733 kb on - strand at 174.003 kb on - strand, within GFF145 at 174.003 kb on - strand, within GFF145 at 174.003 kb on - strand, within GFF145 at 174.003 kb on - strand, within GFF145 at 174.056 kb on + strand, within GFF145 at 174.056 kb on + strand, within GFF145 at 174.056 kb on + strand, within GFF145 at 174.056 kb on + strand, within GFF145 at 174.056 kb on + strand, within GFF145 at 174.057 kb on - strand, within GFF145 at 174.057 kb on - strand, within GFF145 at 174.057 kb on - strand, within GFF145 at 174.057 kb on - strand, within GFF145 at 174.057 kb on - strand, within GFF145 at 174.095 kb on + strand, within GFF145 at 174.095 kb on + strand, within GFF145 at 174.095 kb on + strand, within GFF145 at 174.193 kb on + strand, within GFF145 at 174.194 kb on - strand, within GFF145 at 174.194 kb on - strand, within GFF145 at 174.459 kb on - strand, within GFF145 at 174.518 kb on + strand, within GFF145 at 174.518 kb on + strand, within GFF145 at 174.519 kb on - strand, within GFF145 at 174.519 kb on - strand, within GFF145
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 171,583 + -1.4 172,160 + GFF143 0.70 +0.3 172,160 + GFF143 0.70 -0.4 172,161 - GFF143 0.70 -1.6 172,798 + GFF144 0.33 -0.3 172,798 + GFF144 0.33 -0.1 172,798 + GFF144 0.33 -1.0 172,799 - GFF144 0.34 -0.3 172,822 + GFF144 0.35 -0.7 172,822 + GFF144 0.35 +0.3 173,037 + GFF144 0.53 -0.3 173,037 + GFF144 0.53 -0.3 173,038 - GFF144 0.53 -0.3 173,038 - GFF144 0.53 -1.9 173,038 - GFF144 0.53 -0.9 173,140 + GFF144 0.61 +0.5 173,262 - GFF144 0.71 -0.8 173,263 + GFF144 0.71 -0.4 173,263 + GFF144 0.71 -0.2 173,264 - GFF144 0.71 -0.0 173,264 - GFF144 0.71 -2.0 173,264 - GFF144 0.71 -0.3 173,264 - GFF144 0.71 -1.8 173,264 - GFF144 0.71 -1.1 173,455 + GFF144 0.87 -0.3 173,455 + GFF144 0.87 +0.0 173,717 + -1.0 173,717 + -0.7 173,717 + +0.7 173,718 - -0.2 173,732 + +1.5 173,732 + -0.4 173,732 + -0.4 173,733 - -0.5 173,733 - -0.9 173,733 - +0.4 173,733 - -0.9 174,003 - GFF145 0.29 +1.9 174,003 - GFF145 0.29 +0.8 174,003 - GFF145 0.29 -0.2 174,003 - GFF145 0.29 +0.6 174,056 + GFF145 0.33 -0.3 174,056 + GFF145 0.33 -0.1 174,056 + GFF145 0.33 -0.1 174,056 + GFF145 0.33 -0.9 174,056 + GFF145 0.33 -0.0 174,057 - GFF145 0.33 -0.5 174,057 - GFF145 0.33 -0.7 174,057 - GFF145 0.33 -0.6 174,057 - GFF145 0.33 +0.2 174,057 - GFF145 0.33 -0.9 174,095 + GFF145 0.36 +0.7 174,095 + GFF145 0.36 +0.3 174,095 + GFF145 0.36 -0.6 174,193 + GFF145 0.43 -0.6 174,194 - GFF145 0.43 -0.2 174,194 - GFF145 0.43 -0.3 174,459 - GFF145 0.62 -0.5 174,518 + GFF145 0.66 -0.0 174,518 + GFF145 0.66 +0.9 174,519 - GFF145 0.66 -0.7 174,519 - GFF145 0.66 -0.5
Or see this region's nucleotide sequence