Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1427 and GFF1428 overlap by 4 nucleotides GFF1428 and GFF1429 are separated by 126 nucleotides GFF1429 and GFF1430 are separated by 116 nucleotides
GFF1427 - Mandelate racemase (EC 5.1.2.2), at 17,712 to 18,788
GFF1427
GFF1428 - no description, at 18,785 to 18,940
GFF1428
GFF1429 - no description, at 19,067 to 19,438
GFF1429
GFF1430 - hypothetical protein, at 19,555 to 21,840
GFF1430
Position (kb) 18
19 Strain fitness (log2 ratio) -2
-1
0
1 at 17.822 kb on - strand, within GFF1427 at 17.859 kb on + strand, within GFF1427 at 17.859 kb on + strand, within GFF1427 at 17.860 kb on - strand, within GFF1427 at 17.860 kb on - strand, within GFF1427 at 17.860 kb on - strand, within GFF1427 at 17.872 kb on - strand, within GFF1427 at 17.967 kb on + strand, within GFF1427 at 17.968 kb on - strand, within GFF1427 at 18.436 kb on + strand, within GFF1427 at 18.600 kb on - strand, within GFF1427 at 18.970 kb on + strand at 18.971 kb on - strand at 18.971 kb on - strand at 18.971 kb on - strand at 19.222 kb on - strand, within GFF1429 at 19.222 kb on - strand, within GFF1429 at 19.305 kb on + strand, within GFF1429 at 19.305 kb on + strand, within GFF1429 at 19.608 kb on + strand at 19.609 kb on - strand at 19.609 kb on - strand at 19.609 kb on - strand at 19.698 kb on - strand at 19.698 kb on - strand at 19.698 kb on - strand at 19.850 kb on + strand, within GFF1430 at 19.851 kb on - strand, within GFF1430 at 19.851 kb on - strand, within GFF1430 at 19.856 kb on + strand, within GFF1430 at 19.935 kb on - strand, within GFF1430
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 17,822 - GFF1427 0.10 +0.5 17,859 + GFF1427 0.14 +0.1 17,859 + GFF1427 0.14 +0.4 17,860 - GFF1427 0.14 +1.7 17,860 - GFF1427 0.14 +0.0 17,860 - GFF1427 0.14 +1.1 17,872 - GFF1427 0.15 -2.0 17,967 + GFF1427 0.24 -0.2 17,968 - GFF1427 0.24 -0.3 18,436 + GFF1427 0.67 -0.6 18,600 - GFF1427 0.82 -0.1 18,970 + +0.9 18,971 - +0.3 18,971 - +0.5 18,971 - -0.3 19,222 - GFF1429 0.42 -1.3 19,222 - GFF1429 0.42 -0.5 19,305 + GFF1429 0.64 -0.7 19,305 + GFF1429 0.64 -0.7 19,608 + +1.1 19,609 - +0.2 19,609 - +1.2 19,609 - -0.2 19,698 - -0.6 19,698 - +0.5 19,698 - -0.4 19,850 + GFF1430 0.13 -0.2 19,851 - GFF1430 0.13 -0.3 19,851 - GFF1430 0.13 -0.9 19,856 + GFF1430 0.13 -1.2 19,935 - GFF1430 0.17 +0.7
Or see this region's nucleotide sequence