Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1335 and GFF1336 are separated by 142 nucleotides GFF1336 and GFF1337 are separated by 218 nucleotides
GFF1335 - 3-methylmercaptopropionyl-CoA ligase (EC 6.2.1.44) of DmdB2 type, at 675,402 to 677,045
GFF1335
GFF1336 - ABC transporter, substrate-binding protein RSc1750, at 677,188 to 678,417
GFF1336
GFF1337 - ABC transporter, substrate-binding protein RSc1750, at 678,636 to 679,868
GFF1337
Position (kb)
677
678
679 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 676.356 kb on + strand, within GFF1335 at 676.356 kb on + strand, within GFF1335 at 676.356 kb on + strand, within GFF1335 at 676.357 kb on - strand, within GFF1335 at 676.357 kb on - strand, within GFF1335 at 676.357 kb on - strand, within GFF1335 at 676.357 kb on - strand, within GFF1335 at 676.357 kb on - strand, within GFF1335 at 676.558 kb on - strand, within GFF1335 at 676.599 kb on + strand, within GFF1335 at 676.599 kb on + strand, within GFF1335 at 676.599 kb on + strand, within GFF1335 at 676.599 kb on + strand, within GFF1335 at 676.599 kb on + strand, within GFF1335 at 676.599 kb on + strand, within GFF1335 at 676.599 kb on + strand, within GFF1335 at 676.599 kb on + strand, within GFF1335 at 676.600 kb on - strand, within GFF1335 at 676.600 kb on - strand, within GFF1335 at 676.600 kb on - strand, within GFF1335 at 676.696 kb on - strand, within GFF1335 at 676.696 kb on - strand, within GFF1335 at 676.908 kb on + strand at 676.909 kb on - strand at 676.909 kb on - strand at 676.909 kb on - strand at 676.909 kb on - strand at 676.909 kb on - strand at 677.025 kb on + strand at 677.025 kb on + strand at 677.025 kb on + strand at 677.025 kb on + strand at 677.025 kb on + strand at 677.025 kb on + strand at 677.025 kb on + strand at 677.025 kb on + strand at 677.026 kb on - strand at 677.026 kb on - strand at 677.026 kb on - strand at 677.150 kb on + strand at 677.150 kb on + strand at 677.150 kb on + strand at 677.151 kb on - strand at 677.151 kb on - strand at 677.151 kb on - strand at 677.151 kb on - strand at 677.210 kb on + strand at 677.210 kb on + strand at 677.211 kb on - strand at 677.223 kb on + strand at 677.223 kb on + strand at 677.223 kb on + strand at 677.224 kb on - strand at 677.238 kb on + strand at 677.474 kb on - strand, within GFF1336 at 677.566 kb on + strand, within GFF1336 at 677.566 kb on + strand, within GFF1336 at 677.566 kb on + strand, within GFF1336 at 677.567 kb on - strand, within GFF1336 at 677.567 kb on - strand, within GFF1336 at 677.567 kb on - strand, within GFF1336 at 677.567 kb on - strand, within GFF1336 at 677.567 kb on - strand, within GFF1336 at 677.567 kb on - strand, within GFF1336 at 677.567 kb on - strand, within GFF1336 at 677.575 kb on + strand, within GFF1336 at 677.575 kb on + strand, within GFF1336 at 677.575 kb on + strand, within GFF1336 at 677.575 kb on + strand, within GFF1336 at 677.617 kb on + strand, within GFF1336 at 677.617 kb on + strand, within GFF1336 at 677.617 kb on + strand, within GFF1336 at 677.617 kb on + strand, within GFF1336 at 677.617 kb on + strand, within GFF1336 at 677.618 kb on - strand, within GFF1336 at 677.618 kb on - strand, within GFF1336 at 677.707 kb on + strand, within GFF1336 at 677.708 kb on - strand, within GFF1336 at 677.708 kb on - strand, within GFF1336 at 677.725 kb on + strand, within GFF1336 at 677.752 kb on + strand, within GFF1336 at 677.752 kb on + strand, within GFF1336 at 677.752 kb on + strand, within GFF1336 at 677.753 kb on - strand, within GFF1336 at 677.753 kb on - strand, within GFF1336 at 677.753 kb on - strand, within GFF1336 at 677.845 kb on + strand, within GFF1336 at 677.846 kb on - strand, within GFF1336 at 677.846 kb on - strand, within GFF1336 at 677.965 kb on + strand, within GFF1336 at 677.965 kb on + strand, within GFF1336 at 677.965 kb on + strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.968 kb on + strand, within GFF1336 at 677.968 kb on + strand, within GFF1336 at 677.968 kb on + strand, within GFF1336 at 677.968 kb on + strand, within GFF1336 at 677.969 kb on - strand, within GFF1336 at 677.969 kb on - strand, within GFF1336 at 677.969 kb on - strand, within GFF1336 at 678.026 kb on - strand, within GFF1336 at 678.026 kb on - strand, within GFF1336 at 678.037 kb on + strand, within GFF1336 at 678.037 kb on + strand, within GFF1336 at 678.038 kb on - strand, within GFF1336 at 678.046 kb on + strand, within GFF1336 at 678.046 kb on + strand, within GFF1336 at 678.046 kb on + strand, within GFF1336 at 678.046 kb on + strand, within GFF1336 at 678.047 kb on - strand, within GFF1336 at 678.047 kb on - strand, within GFF1336 at 678.047 kb on - strand, within GFF1336 at 678.086 kb on - strand, within GFF1336 at 678.127 kb on + strand, within GFF1336 at 678.127 kb on + strand, within GFF1336 at 678.127 kb on + strand, within GFF1336 at 678.128 kb on - strand, within GFF1336 at 678.128 kb on - strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.313 kb on + strand at 678.313 kb on + strand at 678.314 kb on - strand at 678.314 kb on - strand at 678.314 kb on - strand at 678.314 kb on - strand at 678.361 kb on + strand at 678.361 kb on + strand at 678.361 kb on + strand at 678.362 kb on - strand at 678.364 kb on + strand at 678.364 kb on + strand at 678.365 kb on - strand at 678.365 kb on - strand at 678.365 kb on - strand at 678.365 kb on - strand at 678.373 kb on + strand at 678.374 kb on - strand at 678.374 kb on - strand at 678.542 kb on + strand at 678.542 kb on + strand at 678.542 kb on + strand at 678.581 kb on - strand at 678.581 kb on - strand at 678.588 kb on + strand at 678.589 kb on - strand at 678.630 kb on + strand at 678.630 kb on + strand at 678.630 kb on + strand at 678.631 kb on - strand at 678.631 kb on - strand at 678.631 kb on - strand at 678.631 kb on - strand at 678.631 kb on - strand at 678.632 kb on + strand at 678.632 kb on + strand at 678.632 kb on + strand at 678.632 kb on + strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.922 kb on - strand, within GFF1337 at 678.922 kb on - strand, within GFF1337 at 679.014 kb on + strand, within GFF1337 at 679.015 kb on - strand, within GFF1337 at 679.065 kb on + strand, within GFF1337 at 679.066 kb on - strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.156 kb on - strand, within GFF1337 at 679.156 kb on - strand, within GFF1337 at 679.156 kb on - strand, within GFF1337 at 679.156 kb on - strand, within GFF1337 at 679.173 kb on + strand, within GFF1337 at 679.174 kb on - strand, within GFF1337 at 679.200 kb on + strand, within GFF1337 at 679.200 kb on + strand, within GFF1337 at 679.201 kb on - strand, within GFF1337 at 679.201 kb on - strand, within GFF1337 at 679.201 kb on - strand, within GFF1337 at 679.293 kb on + strand, within GFF1337 at 679.294 kb on - strand, within GFF1337 at 679.407 kb on + strand, within GFF1337 at 679.407 kb on + strand, within GFF1337 at 679.408 kb on - strand, within GFF1337 at 679.408 kb on - strand, within GFF1337 at 679.413 kb on + strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.416 kb on + strand, within GFF1337 at 679.416 kb on + strand, within GFF1337
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 676,356 + GFF1335 0.58 -0.5 676,356 + GFF1335 0.58 -0.8 676,356 + GFF1335 0.58 -0.1 676,357 - GFF1335 0.58 +0.1 676,357 - GFF1335 0.58 -1.5 676,357 - GFF1335 0.58 +0.4 676,357 - GFF1335 0.58 +0.1 676,357 - GFF1335 0.58 -1.7 676,558 - GFF1335 0.70 -0.1 676,599 + GFF1335 0.73 -0.6 676,599 + GFF1335 0.73 -0.4 676,599 + GFF1335 0.73 +0.1 676,599 + GFF1335 0.73 +0.0 676,599 + GFF1335 0.73 +0.2 676,599 + GFF1335 0.73 -1.3 676,599 + GFF1335 0.73 -1.0 676,599 + GFF1335 0.73 -0.1 676,600 - GFF1335 0.73 -2.2 676,600 - GFF1335 0.73 +0.3 676,600 - GFF1335 0.73 +0.2 676,696 - GFF1335 0.79 -0.0 676,696 - GFF1335 0.79 +0.3 676,908 + +0.4 676,909 - +0.0 676,909 - -2.4 676,909 - -0.6 676,909 - -0.1 676,909 - +0.6 677,025 + -1.7 677,025 + +0.2 677,025 + -0.8 677,025 + -1.0 677,025 + -0.7 677,025 + -0.7 677,025 + +1.5 677,025 + +0.4 677,026 - +0.9 677,026 - -0.4 677,026 - +0.1 677,150 + +0.3 677,150 + -1.1 677,150 + +0.0 677,151 - -1.8 677,151 - -1.4 677,151 - +1.3 677,151 - +0.5 677,210 + -1.5 677,210 + -1.5 677,211 - -2.4 677,223 + +0.2 677,223 + -0.8 677,223 + +0.0 677,224 - -1.7 677,238 + -0.3 677,474 - GFF1336 0.23 -0.9 677,566 + GFF1336 0.31 -0.2 677,566 + GFF1336 0.31 +1.5 677,566 + GFF1336 0.31 -0.3 677,567 - GFF1336 0.31 -0.0 677,567 - GFF1336 0.31 +0.1 677,567 - GFF1336 0.31 +0.5 677,567 - GFF1336 0.31 -0.6 677,567 - GFF1336 0.31 +0.2 677,567 - GFF1336 0.31 +0.8 677,567 - GFF1336 0.31 +0.3 677,575 + GFF1336 0.31 +2.1 677,575 + GFF1336 0.31 +0.1 677,575 + GFF1336 0.31 -0.5 677,575 + GFF1336 0.31 +0.8 677,617 + GFF1336 0.35 +0.1 677,617 + GFF1336 0.35 -0.4 677,617 + GFF1336 0.35 -0.2 677,617 + GFF1336 0.35 +0.3 677,617 + GFF1336 0.35 -0.4 677,618 - GFF1336 0.35 -1.6 677,618 - GFF1336 0.35 +1.2 677,707 + GFF1336 0.42 -0.4 677,708 - GFF1336 0.42 +0.2 677,708 - GFF1336 0.42 -0.2 677,725 + GFF1336 0.44 -0.2 677,752 + GFF1336 0.46 -0.0 677,752 + GFF1336 0.46 +0.6 677,752 + GFF1336 0.46 +0.4 677,753 - GFF1336 0.46 -0.5 677,753 - GFF1336 0.46 -1.1 677,753 - GFF1336 0.46 -0.9 677,845 + GFF1336 0.53 -0.2 677,846 - GFF1336 0.53 +0.1 677,846 - GFF1336 0.53 -0.8 677,965 + GFF1336 0.63 -1.2 677,965 + GFF1336 0.63 +1.3 677,965 + GFF1336 0.63 +0.2 677,966 - GFF1336 0.63 +0.6 677,966 - GFF1336 0.63 +1.3 677,966 - GFF1336 0.63 -0.5 677,966 - GFF1336 0.63 +0.1 677,966 - GFF1336 0.63 -0.5 677,968 + GFF1336 0.63 -0.4 677,968 + GFF1336 0.63 +0.9 677,968 + GFF1336 0.63 +0.0 677,968 + GFF1336 0.63 -0.7 677,969 - GFF1336 0.63 +1.3 677,969 - GFF1336 0.63 -0.2 677,969 - GFF1336 0.63 +0.2 678,026 - GFF1336 0.68 +0.3 678,026 - GFF1336 0.68 -1.2 678,037 + GFF1336 0.69 -0.5 678,037 + GFF1336 0.69 -0.2 678,038 - GFF1336 0.69 -0.4 678,046 + GFF1336 0.70 -1.0 678,046 + GFF1336 0.70 -1.4 678,046 + GFF1336 0.70 -0.3 678,046 + GFF1336 0.70 -1.2 678,047 - GFF1336 0.70 +1.1 678,047 - GFF1336 0.70 -1.2 678,047 - GFF1336 0.70 -0.0 678,086 - GFF1336 0.73 +0.2 678,127 + GFF1336 0.76 +0.6 678,127 + GFF1336 0.76 -0.2 678,127 + GFF1336 0.76 -1.0 678,128 - GFF1336 0.76 +0.8 678,128 - GFF1336 0.76 -1.6 678,280 + GFF1336 0.89 +0.5 678,280 + GFF1336 0.89 +0.1 678,280 + GFF1336 0.89 -0.6 678,280 + GFF1336 0.89 -0.6 678,280 + GFF1336 0.89 -0.5 678,280 + GFF1336 0.89 +0.7 678,280 + GFF1336 0.89 -0.4 678,280 + GFF1336 0.89 +0.9 678,281 - GFF1336 0.89 -0.4 678,281 - GFF1336 0.89 +0.0 678,281 - GFF1336 0.89 +0.1 678,281 - GFF1336 0.89 -0.2 678,281 - GFF1336 0.89 -0.7 678,281 - GFF1336 0.89 +0.2 678,281 - GFF1336 0.89 -0.7 678,281 - GFF1336 0.89 +0.8 678,281 - GFF1336 0.89 +2.9 678,313 + -0.0 678,313 + +1.8 678,314 - +0.9 678,314 - -0.4 678,314 - -0.7 678,314 - +0.3 678,361 + -0.4 678,361 + -0.9 678,361 + -0.8 678,362 - +0.3 678,364 + -0.1 678,364 + -0.9 678,365 - +1.3 678,365 - -0.0 678,365 - -0.6 678,365 - -1.5 678,373 + +0.2 678,374 - -0.2 678,374 - -0.2 678,542 + -0.3 678,542 + +0.1 678,542 + +0.3 678,581 - -0.8 678,581 - -0.8 678,588 + +0.9 678,589 - +0.6 678,630 + -0.2 678,630 + -2.6 678,630 + +0.6 678,631 - +0.2 678,631 - +0.2 678,631 - -0.5 678,631 - -0.1 678,631 - -0.5 678,632 + -0.7 678,632 + -0.9 678,632 + +0.0 678,632 + -0.1 678,633 - +0.3 678,633 - -0.3 678,633 - +0.1 678,633 - +2.1 678,633 - +0.2 678,633 - +1.3 678,633 - +1.3 678,633 - -0.9 678,922 - GFF1337 0.23 +0.3 678,922 - GFF1337 0.23 +1.9 679,014 + GFF1337 0.31 -3.2 679,015 - GFF1337 0.31 +0.3 679,065 + GFF1337 0.35 -0.7 679,066 - GFF1337 0.35 -0.2 679,122 + GFF1337 0.39 -1.0 679,122 + GFF1337 0.39 -0.6 679,122 + GFF1337 0.39 +0.2 679,122 + GFF1337 0.39 -2.0 679,122 + GFF1337 0.39 +0.0 679,122 + GFF1337 0.39 -0.2 679,122 + GFF1337 0.39 -0.6 679,123 - GFF1337 0.39 -0.2 679,123 - GFF1337 0.39 -0.2 679,123 - GFF1337 0.39 +0.8 679,123 - GFF1337 0.39 +0.4 679,123 - GFF1337 0.39 -0.2 679,123 - GFF1337 0.39 -0.0 679,123 - GFF1337 0.39 -0.6 679,156 - GFF1337 0.42 +0.8 679,156 - GFF1337 0.42 +0.2 679,156 - GFF1337 0.42 +0.3 679,156 - GFF1337 0.42 +0.7 679,173 + GFF1337 0.44 -0.1 679,174 - GFF1337 0.44 -0.4 679,200 + GFF1337 0.46 +0.8 679,200 + GFF1337 0.46 +0.1 679,201 - GFF1337 0.46 -0.5 679,201 - GFF1337 0.46 -1.5 679,201 - GFF1337 0.46 -0.2 679,293 + GFF1337 0.53 -1.1 679,294 - GFF1337 0.53 -0.4 679,407 + GFF1337 0.63 +0.7 679,407 + GFF1337 0.63 -0.6 679,408 - GFF1337 0.63 +0.8 679,408 - GFF1337 0.63 +0.2 679,413 + GFF1337 0.63 -0.1 679,414 - GFF1337 0.63 -1.8 679,414 - GFF1337 0.63 -1.9 679,414 - GFF1337 0.63 +0.1 679,414 - GFF1337 0.63 +0.0 679,414 - GFF1337 0.63 -1.5 679,416 + GFF1337 0.63 -1.3 679,416 + GFF1337 0.63 -0.3
Or see this region's nucleotide sequence