Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1245 and GFF1246 are separated by 7 nucleotides GFF1246 and GFF1247 are separated by 137 nucleotides GFF1247 and GFF1248 are separated by 127 nucleotides GFF1248 and GFF1249 are separated by 123 nucleotides
GFF1245 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 564,316 to 566,157
GFF1245
GFF1246 - FIG001341: Probable Fe(2+)-trafficking protein YggX, at 566,165 to 566,437
GFF1246
GFF1247 - Sulfate and thiosulfate binding protein CysP, at 566,575 to 567,615
GFF1247
GFF1248 - FMN reductase (NADPH) (EC 1.5.1.38), at 567,743 to 568,327
GFF1248
GFF1249 - hypothetical protein, at 568,451 to 568,588
GFF1249
Position (kb) 566
567
568 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 565.844 kb on + strand, within GFF1245 at 565.845 kb on - strand, within GFF1245 at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.093 kb on - strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.244 kb on + strand, within GFF1246 at 566.244 kb on + strand, within GFF1246 at 566.244 kb on + strand, within GFF1246 at 566.244 kb on + strand, within GFF1246 at 566.245 kb on - strand, within GFF1246 at 566.245 kb on - strand, within GFF1246 at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.483 kb on + strand at 566.483 kb on + strand at 566.487 kb on + strand at 566.487 kb on + strand at 566.488 kb on - strand at 566.516 kb on + strand at 566.518 kb on - strand at 566.518 kb on - strand at 566.519 kb on + strand at 566.519 kb on + strand at 566.520 kb on - strand at 566.520 kb on - strand at 566.520 kb on - strand at 566.526 kb on + strand at 566.527 kb on - strand at 566.535 kb on + strand at 566.535 kb on + strand at 566.535 kb on + strand at 566.552 kb on - strand at 566.552 kb on - strand at 566.561 kb on + strand at 566.562 kb on - strand at 566.595 kb on + strand at 566.595 kb on + strand at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.702 kb on - strand, within GFF1247 at 566.702 kb on - strand, within GFF1247 at 566.702 kb on - strand, within GFF1247 at 566.702 kb on - strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.723 kb on - strand, within GFF1247 at 566.723 kb on - strand, within GFF1247 at 566.723 kb on - strand, within GFF1247 at 566.723 kb on - strand, within GFF1247 at 566.731 kb on + strand, within GFF1247 at 566.731 kb on + strand, within GFF1247 at 566.731 kb on + strand, within GFF1247 at 566.732 kb on - strand, within GFF1247 at 566.732 kb on - strand, within GFF1247 at 566.732 kb on - strand, within GFF1247 at 566.732 kb on - strand, within GFF1247 at 566.752 kb on + strand, within GFF1247 at 566.752 kb on + strand, within GFF1247 at 566.753 kb on - strand, within GFF1247 at 566.753 kb on - strand, within GFF1247 at 566.753 kb on - strand, within GFF1247 at 566.753 kb on - strand, within GFF1247 at 566.953 kb on + strand, within GFF1247 at 566.953 kb on + strand, within GFF1247 at 566.953 kb on + strand, within GFF1247 at 566.953 kb on + strand, within GFF1247 at 566.954 kb on - strand, within GFF1247 at 566.954 kb on - strand, within GFF1247 at 566.954 kb on - strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.086 kb on - strand, within GFF1247 at 567.086 kb on - strand, within GFF1247 at 567.118 kb on + strand, within GFF1247 at 567.118 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.375 kb on + strand, within GFF1247 at 567.397 kb on + strand, within GFF1247 at 567.397 kb on + strand, within GFF1247 at 567.397 kb on + strand, within GFF1247 at 567.398 kb on - strand, within GFF1247 at 567.398 kb on - strand, within GFF1247 at 567.398 kb on - strand, within GFF1247 at 567.406 kb on + strand, within GFF1247 at 567.406 kb on + strand, within GFF1247 at 567.406 kb on + strand, within GFF1247 at 567.406 kb on + strand, within GFF1247 at 567.406 kb on + strand, within GFF1247 at 567.407 kb on - strand, within GFF1247 at 567.407 kb on - strand, within GFF1247 at 567.407 kb on - strand, within GFF1247 at 567.407 kb on - strand, within GFF1247 at 567.407 kb on - strand, within GFF1247 at 567.412 kb on + strand, within GFF1247 at 567.412 kb on + strand, within GFF1247 at 567.412 kb on + strand, within GFF1247 at 567.413 kb on - strand, within GFF1247 at 567.413 kb on - strand, within GFF1247 at 567.452 kb on - strand, within GFF1247 at 567.452 kb on - strand, within GFF1247 at 567.484 kb on + strand, within GFF1247 at 567.484 kb on + strand, within GFF1247 at 567.485 kb on - strand, within GFF1247 at 567.485 kb on - strand, within GFF1247 at 567.485 kb on - strand, within GFF1247 at 567.595 kb on + strand at 567.595 kb on + strand at 567.596 kb on - strand at 567.596 kb on - strand at 567.971 kb on + strand, within GFF1248 at 567.971 kb on + strand, within GFF1248 at 567.971 kb on + strand, within GFF1248 at 567.972 kb on - strand, within GFF1248 at 567.972 kb on - strand, within GFF1248 at 567.972 kb on - strand, within GFF1248 at 567.983 kb on + strand, within GFF1248 at 567.983 kb on + strand, within GFF1248 at 567.983 kb on + strand, within GFF1248 at 567.984 kb on - strand, within GFF1248 at 568.098 kb on - strand, within GFF1248 at 568.098 kb on - strand, within GFF1248 at 568.154 kb on + strand, within GFF1248 at 568.154 kb on + strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.155 kb on - strand, within GFF1248 at 568.196 kb on + strand, within GFF1248 at 568.196 kb on + strand, within GFF1248 at 568.318 kb on + strand at 568.318 kb on + strand at 568.319 kb on - strand at 568.319 kb on - strand at 568.319 kb on - strand at 568.319 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 565,844 + GFF1245 0.83 +1.0 565,845 - GFF1245 0.83 +0.0 566,090 + -0.6 566,090 + -0.1 566,090 + -0.4 566,090 + +0.4 566,090 + -0.0 566,090 + -0.3 566,090 + -0.6 566,090 + +0.2 566,090 + -1.8 566,090 + -1.2 566,090 + -0.3 566,090 + -0.3 566,090 + +1.5 566,090 + -0.3 566,090 + -1.0 566,090 + -0.6 566,090 + -0.4 566,090 + +0.1 566,090 + +0.6 566,090 + -0.6 566,091 - +1.4 566,091 - +0.2 566,091 - -0.1 566,091 - -0.2 566,091 - -0.5 566,091 - +0.5 566,091 - -0.7 566,091 - +0.4 566,091 - -1.1 566,091 - +0.1 566,091 - -0.3 566,091 - +1.1 566,091 - +1.4 566,091 - -0.5 566,091 - -0.9 566,091 - -0.2 566,091 - -0.2 566,091 - -0.7 566,091 - +0.4 566,093 - -0.1 566,184 + +0.2 566,184 + +1.5 566,184 + -1.0 566,184 + -0.1 566,184 + -0.8 566,184 + +0.2 566,184 + -0.5 566,184 + -1.0 566,184 + -0.3 566,185 - -0.1 566,185 - -1.1 566,185 - -0.4 566,185 - +0.1 566,185 - -0.3 566,185 - -0.6 566,185 - +0.2 566,185 - -0.0 566,185 - -1.2 566,185 - -0.5 566,185 - +0.2 566,244 + GFF1246 0.29 -0.7 566,244 + GFF1246 0.29 -0.6 566,244 + GFF1246 0.29 -1.8 566,244 + GFF1246 0.29 +0.0 566,245 - GFF1246 0.29 +0.2 566,245 - GFF1246 0.29 -1.2 566,470 + +0.4 566,470 + -1.0 566,470 + -0.2 566,470 + -0.0 566,470 + +0.8 566,470 + +1.4 566,470 + +1.4 566,470 + -0.1 566,470 + +0.5 566,470 + -3.2 566,470 + +0.5 566,471 - +0.4 566,471 - -0.5 566,471 - -0.2 566,471 - -1.1 566,471 - -1.8 566,471 - +0.8 566,483 + -0.8 566,483 + -0.8 566,487 + -0.8 566,487 + +0.0 566,488 - +0.3 566,516 + -0.5 566,518 - -1.9 566,518 - +0.9 566,519 + +0.7 566,519 + +0.3 566,520 - +0.4 566,520 - +0.4 566,520 - +0.9 566,526 + +0.7 566,527 - -0.3 566,535 + -1.7 566,535 + -0.0 566,535 + +0.5 566,552 - -0.4 566,552 - -1.2 566,561 + -0.2 566,562 - -0.6 566,595 + -1.5 566,595 + -0.6 566,701 + GFF1247 0.12 +0.3 566,701 + GFF1247 0.12 +0.2 566,701 + GFF1247 0.12 -0.3 566,701 + GFF1247 0.12 -0.6 566,701 + GFF1247 0.12 +0.6 566,701 + GFF1247 0.12 +0.3 566,701 + GFF1247 0.12 -0.1 566,702 - GFF1247 0.12 -1.1 566,702 - GFF1247 0.12 -1.7 566,702 - GFF1247 0.12 +0.5 566,702 - GFF1247 0.12 +0.0 566,722 + GFF1247 0.14 -0.3 566,722 + GFF1247 0.14 -1.5 566,722 + GFF1247 0.14 -0.5 566,722 + GFF1247 0.14 -1.0 566,722 + GFF1247 0.14 +1.4 566,722 + GFF1247 0.14 -0.5 566,722 + GFF1247 0.14 +0.7 566,722 + GFF1247 0.14 -0.9 566,722 + GFF1247 0.14 -0.8 566,722 + GFF1247 0.14 -0.5 566,723 - GFF1247 0.14 +0.4 566,723 - GFF1247 0.14 -0.6 566,723 - GFF1247 0.14 -0.4 566,723 - GFF1247 0.14 -0.0 566,731 + GFF1247 0.15 -0.2 566,731 + GFF1247 0.15 -0.7 566,731 + GFF1247 0.15 -0.4 566,732 - GFF1247 0.15 -0.3 566,732 - GFF1247 0.15 +0.1 566,732 - GFF1247 0.15 +1.4 566,732 - GFF1247 0.15 +0.0 566,752 + GFF1247 0.17 -0.1 566,752 + GFF1247 0.17 -0.8 566,753 - GFF1247 0.17 -1.2 566,753 - GFF1247 0.17 +0.4 566,753 - GFF1247 0.17 -0.4 566,753 - GFF1247 0.17 +0.1 566,953 + GFF1247 0.36 -0.8 566,953 + GFF1247 0.36 -0.0 566,953 + GFF1247 0.36 -0.8 566,953 + GFF1247 0.36 -0.8 566,954 - GFF1247 0.36 -0.6 566,954 - GFF1247 0.36 -0.0 566,954 - GFF1247 0.36 -0.3 567,085 + GFF1247 0.49 -0.2 567,085 + GFF1247 0.49 -0.2 567,085 + GFF1247 0.49 +0.8 567,085 + GFF1247 0.49 -0.9 567,085 + GFF1247 0.49 -0.5 567,085 + GFF1247 0.49 +0.4 567,086 - GFF1247 0.49 -0.7 567,086 - GFF1247 0.49 -0.2 567,118 + GFF1247 0.52 +1.0 567,118 + GFF1247 0.52 -0.2 567,145 + GFF1247 0.55 -1.1 567,145 + GFF1247 0.55 -0.5 567,145 + GFF1247 0.55 +2.4 567,145 + GFF1247 0.55 -0.8 567,145 + GFF1247 0.55 +0.0 567,145 + GFF1247 0.55 +0.4 567,146 - GFF1247 0.55 -2.3 567,146 - GFF1247 0.55 -2.1 567,146 - GFF1247 0.55 -0.5 567,146 - GFF1247 0.55 +0.9 567,146 - GFF1247 0.55 -1.4 567,146 - GFF1247 0.55 -0.3 567,375 + GFF1247 0.77 -0.2 567,397 + GFF1247 0.79 -0.6 567,397 + GFF1247 0.79 +1.4 567,397 + GFF1247 0.79 +0.6 567,398 - GFF1247 0.79 +0.2 567,398 - GFF1247 0.79 -1.8 567,398 - GFF1247 0.79 +0.6 567,406 + GFF1247 0.80 +2.9 567,406 + GFF1247 0.80 -0.2 567,406 + GFF1247 0.80 +0.1 567,406 + GFF1247 0.80 +0.6 567,406 + GFF1247 0.80 +1.4 567,407 - GFF1247 0.80 -0.4 567,407 - GFF1247 0.80 -0.3 567,407 - GFF1247 0.80 -0.8 567,407 - GFF1247 0.80 -0.1 567,407 - GFF1247 0.80 -0.1 567,412 + GFF1247 0.80 -0.2 567,412 + GFF1247 0.80 +0.9 567,412 + GFF1247 0.80 +1.2 567,413 - GFF1247 0.80 -1.0 567,413 - GFF1247 0.80 -0.2 567,452 - GFF1247 0.84 -0.9 567,452 - GFF1247 0.84 +0.4 567,484 + GFF1247 0.87 -0.5 567,484 + GFF1247 0.87 -0.2 567,485 - GFF1247 0.87 -0.0 567,485 - GFF1247 0.87 +0.6 567,485 - GFF1247 0.87 -1.1 567,595 + +1.4 567,595 + -0.2 567,596 - +0.4 567,596 - +0.4 567,971 + GFF1248 0.39 -1.2 567,971 + GFF1248 0.39 +0.0 567,971 + GFF1248 0.39 +0.4 567,972 - GFF1248 0.39 -0.8 567,972 - GFF1248 0.39 +0.5 567,972 - GFF1248 0.39 +0.4 567,983 + GFF1248 0.41 -1.1 567,983 + GFF1248 0.41 -0.2 567,983 + GFF1248 0.41 -0.8 567,984 - GFF1248 0.41 -0.2 568,098 - GFF1248 0.61 -0.3 568,098 - GFF1248 0.61 -0.2 568,154 + GFF1248 0.70 +0.5 568,154 + GFF1248 0.70 -1.6 568,155 - GFF1248 0.70 -1.0 568,155 - GFF1248 0.70 +1.4 568,155 - GFF1248 0.70 -1.1 568,155 - GFF1248 0.70 -0.2 568,155 - GFF1248 0.70 +0.1 568,155 - GFF1248 0.70 -0.2 568,155 - GFF1248 0.70 -1.3 568,155 - GFF1248 0.70 -1.1 568,155 - GFF1248 0.70 -0.1 568,155 - GFF1248 0.70 -1.2 568,196 + GFF1248 0.77 -1.0 568,196 + GFF1248 0.77 +0.4 568,318 + +0.2 568,318 + -0.6 568,319 - -0.0 568,319 - -0.5 568,319 - -0.9 568,319 - +0.2
Or see this region's nucleotide sequence