Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1150 and GFF1151 overlap by 4 nucleotides GFF1151 and GFF1152 are separated by 47 nucleotides GFF1152 and GFF1153 are separated by 31 nucleotides
GFF1150 - NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase, at 467,475 to 469,172
GFF1150
GFF1151 - Nitrogen regulatory protein P-II, at 469,169 to 469,507
GFF1151
GFF1152 - Lysine decarboxylase family, at 469,555 to 470,145
GFF1152
GFF1153 - Diacylglycerol kinase (EC 2.7.1.107), at 470,177 to 470,584
GFF1153
Position (kb)
469
470 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 468.339 kb on + strand, within GFF1150 at 468.339 kb on + strand, within GFF1150 at 468.340 kb on - strand, within GFF1150 at 468.340 kb on - strand, within GFF1150 at 468.340 kb on - strand, within GFF1150 at 468.340 kb on - strand, within GFF1150 at 468.340 kb on - strand, within GFF1150 at 468.368 kb on + strand, within GFF1150 at 468.472 kb on - strand, within GFF1150 at 468.472 kb on - strand, within GFF1150 at 468.472 kb on - strand, within GFF1150 at 468.531 kb on + strand, within GFF1150 at 468.726 kb on + strand, within GFF1150 at 468.726 kb on + strand, within GFF1150 at 468.738 kb on + strand, within GFF1150 at 468.739 kb on - strand, within GFF1150 at 468.739 kb on - strand, within GFF1150 at 468.930 kb on + strand, within GFF1150 at 468.931 kb on - strand, within GFF1150 at 468.960 kb on + strand, within GFF1150 at 468.960 kb on + strand, within GFF1150 at 468.960 kb on + strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 468.961 kb on - strand, within GFF1150 at 469.005 kb on + strand at 469.056 kb on + strand at 469.056 kb on + strand at 469.056 kb on + strand at 469.056 kb on + strand at 469.056 kb on + strand at 469.056 kb on + strand at 469.056 kb on + strand at 469.057 kb on - strand at 469.057 kb on - strand at 469.057 kb on - strand at 469.057 kb on - strand at 469.057 kb on - strand at 469.127 kb on + strand at 469.128 kb on - strand at 469.128 kb on - strand at 469.128 kb on - strand at 469.128 kb on - strand at 469.305 kb on - strand, within GFF1151 at 469.319 kb on + strand, within GFF1151 at 469.319 kb on + strand, within GFF1151 at 469.319 kb on + strand, within GFF1151 at 469.319 kb on + strand, within GFF1151 at 469.319 kb on + strand, within GFF1151 at 469.319 kb on + strand, within GFF1151 at 469.319 kb on + strand, within GFF1151 at 469.320 kb on - strand, within GFF1151 at 469.320 kb on - strand, within GFF1151 at 469.320 kb on - strand, within GFF1151 at 469.320 kb on - strand, within GFF1151 at 469.402 kb on + strand, within GFF1151 at 469.402 kb on + strand, within GFF1151 at 469.402 kb on + strand, within GFF1151 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.722 kb on - strand, within GFF1152 at 469.722 kb on - strand, within GFF1152 at 469.724 kb on + strand, within GFF1152 at 469.725 kb on - strand, within GFF1152 at 469.725 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 468,339 + GFF1150 0.51 -1.2 468,339 + GFF1150 0.51 +1.1 468,340 - GFF1150 0.51 +0.6 468,340 - GFF1150 0.51 -0.0 468,340 - GFF1150 0.51 -0.6 468,340 - GFF1150 0.51 -1.4 468,340 - GFF1150 0.51 -0.5 468,368 + GFF1150 0.53 -0.2 468,472 - GFF1150 0.59 +2.1 468,472 - GFF1150 0.59 -0.4 468,472 - GFF1150 0.59 -0.8 468,531 + GFF1150 0.62 -0.1 468,726 + GFF1150 0.74 -0.2 468,726 + GFF1150 0.74 -0.2 468,738 + GFF1150 0.74 +2.8 468,739 - GFF1150 0.74 -0.5 468,739 - GFF1150 0.74 -0.0 468,930 + GFF1150 0.86 -1.1 468,931 - GFF1150 0.86 -0.2 468,960 + GFF1150 0.87 -0.2 468,960 + GFF1150 0.87 +0.4 468,960 + GFF1150 0.87 -0.8 468,961 - GFF1150 0.88 -0.7 468,961 - GFF1150 0.88 +1.8 468,961 - GFF1150 0.88 +0.2 468,961 - GFF1150 0.88 +0.1 468,961 - GFF1150 0.88 -0.6 468,961 - GFF1150 0.88 -1.4 468,961 - GFF1150 0.88 +1.0 468,961 - GFF1150 0.88 -0.3 468,961 - GFF1150 0.88 +0.5 468,961 - GFF1150 0.88 +1.1 469,005 + -0.4 469,056 + -0.7 469,056 + +1.2 469,056 + -0.9 469,056 + -0.6 469,056 + -0.6 469,056 + -1.9 469,056 + -0.7 469,057 - +0.4 469,057 - -1.4 469,057 - -0.6 469,057 - -1.6 469,057 - +0.6 469,127 + -0.9 469,128 - +1.4 469,128 - -0.9 469,128 - +0.9 469,128 - -0.4 469,305 - GFF1151 0.40 -0.2 469,319 + GFF1151 0.44 -0.6 469,319 + GFF1151 0.44 -0.8 469,319 + GFF1151 0.44 +0.3 469,319 + GFF1151 0.44 -0.2 469,319 + GFF1151 0.44 -1.5 469,319 + GFF1151 0.44 -0.6 469,319 + GFF1151 0.44 +0.4 469,320 - GFF1151 0.45 -0.2 469,320 - GFF1151 0.45 -0.4 469,320 - GFF1151 0.45 -0.4 469,320 - GFF1151 0.45 -0.3 469,402 + GFF1151 0.69 -0.7 469,402 + GFF1151 0.69 -0.0 469,402 + GFF1151 0.69 -1.9 469,721 + GFF1152 0.28 -0.2 469,721 + GFF1152 0.28 -0.2 469,721 + GFF1152 0.28 +0.8 469,721 + GFF1152 0.28 -0.9 469,721 + GFF1152 0.28 +0.3 469,721 + GFF1152 0.28 +0.5 469,721 + GFF1152 0.28 -0.3 469,721 + GFF1152 0.28 -0.1 469,722 - GFF1152 0.28 -0.5 469,722 - GFF1152 0.28 +0.4 469,724 + GFF1152 0.29 +1.5 469,725 - GFF1152 0.29 -0.3 469,725 - GFF1152 0.29 -0.9 470,025 - GFF1152 0.80 +2.1 470,025 - GFF1152 0.80 -0.5 470,025 - GFF1152 0.80 -0.5 470,025 - GFF1152 0.80 -0.9 470,025 - GFF1152 0.80 -1.1 470,025 - GFF1152 0.80 -0.9 470,025 - GFF1152 0.80 -1.2 470,117 + +1.4 470,117 + +2.0 470,117 + +1.4 470,117 + +0.4 470,117 + -0.0 470,117 + +0.4 470,117 + -0.0 470,117 + +0.2 470,118 - +0.3 470,118 - -0.3 470,118 - +0.2 470,118 - -0.1 470,118 - -0.8 470,118 - -0.2 470,118 - +0.2
Or see this region's nucleotide sequence