Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF779 and GFF780 are separated by 175 nucleotides GFF780 and GFF781 overlap by 8 nucleotides GFF781 and GFF782 are separated by 5 nucleotides GFF782 and GFF783 are separated by 101 nucleotides
GFF779 - hypothetical protein, at 60,892 to 62,088
GFF779
GFF780 - no description, at 62,264 to 62,692
GFF780
GFF781 - hypothetical protein, at 62,685 to 64,532
GFF781
GFF782 - Alpha-ketoglutarate-dependent dioxygenase AlkB (EC 1.14.11.33), at 64,538 to 65,245
GFF782
GFF783 - no description, at 65,347 to 66,069
GFF783
Position (kb) 62
63
64
65 Strain fitness (log2 ratio) -2
-1
0
1
2 at 61.744 kb on + strand, within GFF779 at 61.744 kb on + strand, within GFF779 at 61.763 kb on - strand, within GFF779 at 61.869 kb on + strand, within GFF779 at 61.869 kb on + strand, within GFF779 at 61.997 kb on - strand at 61.997 kb on - strand at 62.035 kb on + strand at 62.036 kb on - strand at 62.036 kb on - strand at 62.036 kb on - strand at 62.139 kb on + strand at 62.139 kb on + strand at 62.140 kb on - strand at 62.140 kb on - strand at 62.140 kb on - strand at 62.242 kb on + strand at 62.242 kb on + strand at 62.242 kb on + strand at 62.243 kb on - strand at 62.243 kb on - strand at 62.243 kb on - strand at 62.243 kb on - strand at 62.257 kb on - strand at 62.276 kb on + strand at 62.277 kb on - strand at 62.277 kb on - strand at 62.277 kb on - strand at 62.502 kb on - strand, within GFF780 at 62.502 kb on - strand, within GFF780 at 62.546 kb on + strand, within GFF780 at 62.615 kb on + strand, within GFF780 at 62.615 kb on + strand, within GFF780 at 62.615 kb on + strand, within GFF780 at 62.615 kb on + strand, within GFF780 at 62.615 kb on + strand, within GFF780 at 62.616 kb on - strand, within GFF780 at 62.616 kb on - strand, within GFF780 at 62.616 kb on - strand, within GFF780 at 62.616 kb on - strand, within GFF780 at 62.642 kb on - strand, within GFF780 at 62.690 kb on + strand at 62.690 kb on + strand at 62.691 kb on - strand at 62.691 kb on - strand at 62.755 kb on + strand at 63.410 kb on - strand, within GFF781 at 63.559 kb on + strand, within GFF781 at 63.559 kb on + strand, within GFF781 at 63.634 kb on + strand, within GFF781 at 63.640 kb on + strand, within GFF781 at 63.640 kb on + strand, within GFF781 at 63.640 kb on + strand, within GFF781 at 63.641 kb on - strand, within GFF781 at 63.641 kb on - strand, within GFF781 at 63.641 kb on - strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.701 kb on - strand, within GFF781 at 63.701 kb on - strand, within GFF781 at 63.701 kb on - strand, within GFF781 at 63.902 kb on - strand, within GFF781 at 63.985 kb on + strand, within GFF781 at 64.193 kb on - strand, within GFF781 at 64.193 kb on - strand, within GFF781 at 64.193 kb on - strand, within GFF781 at 64.282 kb on + strand, within GFF781 at 64.282 kb on + strand, within GFF781 at 64.283 kb on - strand, within GFF781 at 64.283 kb on - strand, within GFF781 at 64.465 kb on + strand at 64.465 kb on + strand at 64.466 kb on - strand at 64.537 kb on - strand at 64.682 kb on + strand, within GFF782 at 64.683 kb on - strand, within GFF782 at 64.764 kb on + strand, within GFF782 at 64.765 kb on - strand, within GFF782 at 64.815 kb on + strand, within GFF782 at 64.816 kb on - strand, within GFF782 at 65.268 kb on + strand at 65.268 kb on + strand at 65.268 kb on + strand at 65.289 kb on + strand at 65.289 kb on + strand at 65.292 kb on + strand at 65.292 kb on + strand at 65.293 kb on - strand at 65.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 61,744 + GFF779 0.71 +0.2 61,744 + GFF779 0.71 +1.3 61,763 - GFF779 0.73 -0.0 61,869 + GFF779 0.82 +0.4 61,869 + GFF779 0.82 -0.2 61,997 - -0.8 61,997 - +0.4 62,035 + -0.5 62,036 - -0.4 62,036 - +0.1 62,036 - -0.4 62,139 + -1.4 62,139 + -0.7 62,140 - -0.2 62,140 - -0.3 62,140 - +1.8 62,242 + +0.1 62,242 + +1.5 62,242 + -1.8 62,243 - +0.7 62,243 - -0.6 62,243 - -0.0 62,243 - +0.1 62,257 - +0.1 62,276 + -0.4 62,277 - -0.2 62,277 - +0.3 62,277 - +0.6 62,502 - GFF780 0.55 +0.6 62,502 - GFF780 0.55 -1.6 62,546 + GFF780 0.66 +0.5 62,615 + GFF780 0.82 -1.9 62,615 + GFF780 0.82 -0.2 62,615 + GFF780 0.82 -1.1 62,615 + GFF780 0.82 +0.5 62,615 + GFF780 0.82 -1.8 62,616 - GFF780 0.82 -0.0 62,616 - GFF780 0.82 -0.8 62,616 - GFF780 0.82 -0.1 62,616 - GFF780 0.82 +0.3 62,642 - GFF780 0.88 -0.1 62,690 + -0.7 62,690 + -0.2 62,691 - +0.8 62,691 - -0.2 62,755 + +0.4 63,410 - GFF781 0.39 -0.3 63,559 + GFF781 0.47 -0.5 63,559 + GFF781 0.47 -1.5 63,634 + GFF781 0.51 -0.4 63,640 + GFF781 0.52 -2.2 63,640 + GFF781 0.52 +0.6 63,640 + GFF781 0.52 +0.9 63,641 - GFF781 0.52 -0.1 63,641 - GFF781 0.52 -2.0 63,641 - GFF781 0.52 +0.1 63,700 + GFF781 0.55 -0.8 63,700 + GFF781 0.55 +0.3 63,700 + GFF781 0.55 +0.3 63,700 + GFF781 0.55 +0.1 63,700 + GFF781 0.55 -0.8 63,701 - GFF781 0.55 +1.3 63,701 - GFF781 0.55 -0.1 63,701 - GFF781 0.55 -1.2 63,902 - GFF781 0.66 -0.5 63,985 + GFF781 0.70 -0.2 64,193 - GFF781 0.82 +1.8 64,193 - GFF781 0.82 -1.5 64,193 - GFF781 0.82 +1.0 64,282 + GFF781 0.86 +0.7 64,282 + GFF781 0.86 +1.0 64,283 - GFF781 0.86 +0.2 64,283 - GFF781 0.86 +0.5 64,465 + -0.1 64,465 + -0.2 64,466 - -1.3 64,537 - -0.2 64,682 + GFF782 0.20 -0.9 64,683 - GFF782 0.20 +0.2 64,764 + GFF782 0.32 +0.1 64,765 - GFF782 0.32 +0.3 64,815 + GFF782 0.39 +1.8 64,816 - GFF782 0.39 -0.2 65,268 + -0.3 65,268 + -1.0 65,268 + -0.3 65,289 + -0.8 65,289 + -0.9 65,292 + -0.8 65,292 + +0.5 65,293 - -0.0 65,293 - -0.5
Or see this region's nucleotide sequence