Strain Fitness in Variovorax sp. SCN45 around GFF74

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF72 and GFF73 are separated by 2 nucleotidesGFF73 and GFF74 are separated by 72 nucleotidesGFF74 and GFF75 are separated by 106 nucleotides GFF72 - D-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.-), at 89,244 to 90,377 GFF72 GFF73 - Probable haloacid dehalogenase, at 90,380 to 91,039 GFF73 GFF74 - Transcriptional regulator, LysR family, at 91,112 to 92,041 GFF74 GFF75 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 92,148 to 93,164 GFF75 Position (kb) 91 92 93Strain fitness (log2 ratio) -1 0 1at 90.134 kb on - strand, within GFF72at 90.495 kb on + strand, within GFF73at 90.496 kb on - strand, within GFF73at 90.496 kb on - strand, within GFF73at 90.534 kb on + strand, within GFF73at 90.535 kb on - strand, within GFF73at 90.621 kb on + strand, within GFF73at 90.621 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.874 kb on - strand, within GFF73at 90.874 kb on - strand, within GFF73at 90.874 kb on - strand, within GFF73at 90.874 kb on - strand, within GFF73at 90.889 kb on - strand, within GFF73at 90.903 kb on + strand, within GFF73at 91.052 kb on + strandat 91.052 kb on + strandat 91.052 kb on + strandat 91.053 kb on - strandat 91.053 kb on - strandat 91.099 kb on + strandat 91.428 kb on - strand, within GFF74at 91.619 kb on + strand, within GFF74at 91.620 kb on - strand, within GFF74at 91.620 kb on - strand, within GFF74at 91.799 kb on + strand, within GFF74at 91.799 kb on + strand, within GFF74at 91.799 kb on + strand, within GFF74at 91.800 kb on - strand, within GFF74at 91.800 kb on - strand, within GFF74at 91.800 kb on - strand, within GFF74at 91.800 kb on - strand, within GFF74at 91.978 kb on + strandat 91.978 kb on + strandat 92.048 kb on + strandat 92.064 kb on + strandat 92.622 kb on + strand, within GFF75at 92.622 kb on + strand, within GFF75at 92.623 kb on - strand, within GFF75at 92.623 kb on - strand, within GFF75at 92.925 kb on + strand, within GFF75at 92.926 kb on - strand, within GFF75at 92.926 kb on - strand, within GFF75at 92.926 kb on - strand, within GFF75at 92.926 kb on - strand, within GFF75at 92.926 kb on - strand, within GFF75at 92.970 kb on + strand, within GFF75at 92.970 kb on + strand, within GFF75at 92.971 kb on - strand, within GFF75at 93.024 kb on + strand, within GFF75at 93.024 kb on + strand, within GFF75at 93.024 kb on + strand, within GFF75at 93.025 kb on - strand, within GFF75at 93.025 kb on - strand, within GFF75

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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90,134 - GFF72 0.78 +0.0
90,495 + GFF73 0.17 -0.9
90,496 - GFF73 0.18 +0.1
90,496 - GFF73 0.18 -1.0
90,534 + GFF73 0.23 -0.3
90,535 - GFF73 0.23 -1.1
90,621 + GFF73 0.37 -1.0
90,621 + GFF73 0.37 +0.7
90,873 + GFF73 0.75 +0.1
90,873 + GFF73 0.75 +0.6
90,873 + GFF73 0.75 -0.1
90,873 + GFF73 0.75 -0.5
90,873 + GFF73 0.75 -0.3
90,874 - GFF73 0.75 -1.2
90,874 - GFF73 0.75 -0.7
90,874 - GFF73 0.75 -0.4
90,874 - GFF73 0.75 -0.7
90,889 - GFF73 0.77 -0.7
90,903 + GFF73 0.79 -0.2
91,052 + +0.0
91,052 + +0.2
91,052 + -0.7
91,053 - -0.4
91,053 - +0.0
91,099 + -1.5
91,428 - GFF74 0.34 +0.3
91,619 + GFF74 0.55 -0.4
91,620 - GFF74 0.55 +0.6
91,620 - GFF74 0.55 -0.7
91,799 + GFF74 0.74 -0.7
91,799 + GFF74 0.74 -0.0
91,799 + GFF74 0.74 -0.6
91,800 - GFF74 0.74 +0.3
91,800 - GFF74 0.74 -0.3
91,800 - GFF74 0.74 +1.0
91,800 - GFF74 0.74 -0.6
91,978 + -0.2
91,978 + -0.7
92,048 + -0.6
92,064 + +0.9
92,622 + GFF75 0.47 -0.8
92,622 + GFF75 0.47 -0.5
92,623 - GFF75 0.47 +0.6
92,623 - GFF75 0.47 -0.7
92,925 + GFF75 0.76 -0.3
92,926 - GFF75 0.76 +0.4
92,926 - GFF75 0.76 +0.0
92,926 - GFF75 0.76 +0.2
92,926 - GFF75 0.76 +0.3
92,926 - GFF75 0.76 +0.4
92,970 + GFF75 0.81 -0.7
92,970 + GFF75 0.81 -1.4
92,971 - GFF75 0.81 +0.0
93,024 + GFF75 0.86 -0.7
93,024 + GFF75 0.86 -0.5
93,024 + GFF75 0.86 +1.0
93,025 - GFF75 0.86 -0.2
93,025 - GFF75 0.86 -0.7

Or see this region's nucleotide sequence