Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF627 and GFF628 are separated by 107 nucleotides GFF628 and GFF629 overlap by 4 nucleotides GFF629 and GFF630 are separated by 6 nucleotides GFF630 and GFF631 are separated by 16 nucleotides
GFF627 - Secretion protein HlyD, at 676,579 to 677,751
GFF627
GFF628 - hypothetical protein, at 677,859 to 678,527
GFF628
GFF629 - hypothetical protein, at 678,524 to 679,903
GFF629
GFF630 - CxxC_CXXC_SSSS domain protein, FmdB family, at 679,910 to 680,254
GFF630
GFF631 - Formamidase (EC 3.5.1.49), at 680,271 to 681,503
GFF631
Position (kb) 678
679
680 Strain fitness (log2 ratio) -2
-1
0
1
2 at 677.787 kb on + strand at 677.834 kb on + strand at 677.834 kb on + strand at 677.835 kb on - strand at 677.835 kb on - strand at 677.835 kb on - strand at 677.835 kb on - strand at 678.151 kb on - strand, within GFF628 at 678.235 kb on + strand, within GFF628 at 678.435 kb on + strand, within GFF628 at 678.435 kb on + strand, within GFF628 at 678.435 kb on + strand, within GFF628 at 678.435 kb on + strand, within GFF628 at 678.436 kb on - strand, within GFF628 at 678.436 kb on - strand, within GFF628 at 678.436 kb on - strand, within GFF628 at 678.436 kb on - strand, within GFF628 at 678.436 kb on - strand, within GFF628 at 678.504 kb on + strand at 678.893 kb on + strand, within GFF629 at 678.893 kb on + strand, within GFF629 at 678.894 kb on - strand, within GFF629 at 678.894 kb on - strand, within GFF629 at 680.244 kb on + strand at 680.244 kb on + strand at 680.245 kb on - strand at 680.474 kb on - strand, within GFF631 at 680.599 kb on + strand, within GFF631 at 680.599 kb on + strand, within GFF631 at 680.600 kb on - strand, within GFF631 at 680.600 kb on - strand, within GFF631 at 680.600 kb on - strand, within GFF631 at 680.647 kb on + strand, within GFF631 at 680.648 kb on - strand, within GFF631 at 680.648 kb on - strand, within GFF631 at 680.785 kb on + strand, within GFF631 at 680.785 kb on + strand, within GFF631
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 677,787 + -0.6 677,834 + -0.3 677,834 + -0.6 677,835 - -0.1 677,835 - +0.2 677,835 - -1.0 677,835 - +0.5 678,151 - GFF628 0.44 -0.2 678,235 + GFF628 0.56 +0.2 678,435 + GFF628 0.86 +0.5 678,435 + GFF628 0.86 +0.7 678,435 + GFF628 0.86 +1.1 678,435 + GFF628 0.86 -0.6 678,436 - GFF628 0.86 +0.2 678,436 - GFF628 0.86 +2.6 678,436 - GFF628 0.86 -0.2 678,436 - GFF628 0.86 +1.6 678,436 - GFF628 0.86 -2.0 678,504 + +0.3 678,893 + GFF629 0.27 -1.5 678,893 + GFF629 0.27 +0.7 678,894 - GFF629 0.27 -1.4 678,894 - GFF629 0.27 -1.2 680,244 + +0.1 680,244 + -0.0 680,245 - -0.3 680,474 - GFF631 0.16 -0.1 680,599 + GFF631 0.27 -0.9 680,599 + GFF631 0.27 +2.1 680,600 - GFF631 0.27 +0.7 680,600 - GFF631 0.27 +0.6 680,600 - GFF631 0.27 -0.2 680,647 + GFF631 0.30 +0.3 680,648 - GFF631 0.31 +0.8 680,648 - GFF631 0.31 +0.4 680,785 + GFF631 0.42 +1.6 680,785 + GFF631 0.42 +1.0
Or see this region's nucleotide sequence