Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5891 and GFF5892 are separated by 21 nucleotides GFF5892 and GFF5893 are separated by 17 nucleotides GFF5893 and GFF5894 are separated by 5 nucleotides GFF5894 and GFF5895 overlap by 4 nucleotides GFF5895 and GFF5896 are separated by 2 nucleotides
GFF5891 - Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 110,408 to 111,430
GFF5891
GFF5892 - Lysine exporter protein LysE/YggA, at 111,452 to 112,111
GFF5892
GFF5893 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 112,129 to 113,187
GFF5893
GFF5894 - Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85), at 113,193 to 113,777
GFF5894
GFF5895 - hypothetical protein, at 113,774 to 114,166
GFF5895
GFF5896 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 114,169 to 115,671
GFF5896
Position (kb)
112
113
114 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 111.864 kb on + strand, within GFF5892 at 111.864 kb on + strand, within GFF5892 at 111.879 kb on + strand, within GFF5892 at 111.879 kb on + strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 112.032 kb on + strand, within GFF5892 at 112.032 kb on + strand, within GFF5892 at 112.032 kb on + strand, within GFF5892 at 112.032 kb on + strand, within GFF5892 at 112.033 kb on - strand, within GFF5892 at 112.217 kb on + strand at 112.218 kb on - strand at 112.727 kb on + strand, within GFF5893 at 112.728 kb on - strand, within GFF5893 at 112.728 kb on - strand, within GFF5893 at 112.943 kb on + strand, within GFF5893 at 112.943 kb on + strand, within GFF5893 at 112.943 kb on + strand, within GFF5893 at 112.944 kb on - strand, within GFF5893 at 112.944 kb on - strand, within GFF5893 at 112.944 kb on - strand, within GFF5893 at 112.944 kb on - strand, within GFF5893 at 112.944 kb on - strand, within GFF5893 at 113.752 kb on + strand at 113.753 kb on - strand at 113.753 kb on - strand at 113.753 kb on - strand at 113.961 kb on + strand, within GFF5895 at 113.961 kb on + strand, within GFF5895 at 113.989 kb on - strand, within GFF5895 at 113.989 kb on - strand, within GFF5895 at 113.989 kb on - strand, within GFF5895 at 113.989 kb on - strand, within GFF5895 at 113.989 kb on - strand, within GFF5895 at 114.063 kb on + strand, within GFF5895 at 114.064 kb on - strand, within GFF5895 at 114.170 kb on + strand at 114.170 kb on + strand at 114.170 kb on + strand at 114.170 kb on + strand at 114.170 kb on + strand at 114.171 kb on - strand at 114.171 kb on - strand at 114.171 kb on - strand at 114.171 kb on - strand at 114.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 111,864 + GFF5892 0.62 +0.9 111,864 + GFF5892 0.62 +1.1 111,879 + GFF5892 0.65 -0.3 111,879 + GFF5892 0.65 +0.0 111,880 - GFF5892 0.65 +0.3 111,880 - GFF5892 0.65 +1.4 111,880 - GFF5892 0.65 +0.3 111,880 - GFF5892 0.65 -0.6 111,880 - GFF5892 0.65 +0.1 111,880 - GFF5892 0.65 -1.5 111,880 - GFF5892 0.65 -0.4 112,032 + GFF5892 0.88 +0.5 112,032 + GFF5892 0.88 -0.5 112,032 + GFF5892 0.88 -0.1 112,032 + GFF5892 0.88 +0.1 112,033 - GFF5892 0.88 -0.9 112,217 + +0.3 112,218 - +1.1 112,727 + GFF5893 0.56 -0.2 112,728 - GFF5893 0.57 -2.4 112,728 - GFF5893 0.57 -1.5 112,943 + GFF5893 0.77 +1.4 112,943 + GFF5893 0.77 -0.6 112,943 + GFF5893 0.77 +0.9 112,944 - GFF5893 0.77 -1.1 112,944 - GFF5893 0.77 +0.1 112,944 - GFF5893 0.77 +1.3 112,944 - GFF5893 0.77 -1.9 112,944 - GFF5893 0.77 +0.1 113,752 + -0.5 113,753 - -0.9 113,753 - -1.2 113,753 - -0.7 113,961 + GFF5895 0.48 +0.4 113,961 + GFF5895 0.48 +1.9 113,989 - GFF5895 0.55 -1.2 113,989 - GFF5895 0.55 -1.5 113,989 - GFF5895 0.55 +2.1 113,989 - GFF5895 0.55 -0.1 113,989 - GFF5895 0.55 -0.1 114,063 + GFF5895 0.74 -0.2 114,064 - GFF5895 0.74 +0.5 114,170 + +0.3 114,170 + +0.7 114,170 + -0.3 114,170 + -0.5 114,170 + +0.6 114,171 - +0.5 114,171 - +0.1 114,171 - +0.3 114,171 - -1.1 114,171 - -1.8
Or see this region's nucleotide sequence