Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF54 and GFF55 overlap by 4 nucleotides GFF55 and GFF56 overlap by 8 nucleotides GFF56 and GFF57 are separated by 67 nucleotides
GFF54 - 4'-phosphopantetheinyl transferase (EC 2.7.8.-), at 69,143 to 69,856
GFF54
GFF55 - hypothetical protein, at 69,853 to 70,671
GFF55
GFF56 - hypothetical protein, at 70,664 to 71,593
GFF56
GFF57 - Putative polysaccharide export protein YccZ precursor, at 71,661 to 72,872
GFF57
Position (kb) 70
71
72 Strain fitness (log2 ratio) -2
-1
0
1
2 at 70.057 kb on + strand, within GFF55 at 70.058 kb on - strand, within GFF55 at 70.335 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.571 kb on - strand, within GFF55 at 71.327 kb on + strand, within GFF56 at 71.327 kb on + strand, within GFF56 at 71.328 kb on - strand, within GFF56 at 71.328 kb on - strand, within GFF56 at 71.328 kb on - strand, within GFF56 at 71.412 kb on - strand, within GFF56 at 71.616 kb on + strand at 71.617 kb on - strand at 71.983 kb on - strand, within GFF57 at 71.983 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.141 kb on + strand, within GFF57 at 72.274 kb on - strand, within GFF57 at 72.525 kb on + strand, within GFF57 at 72.526 kb on - strand, within GFF57 at 72.526 kb on - strand, within GFF57
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 70,057 + GFF55 0.25 -0.9 70,058 - GFF55 0.25 +0.0 70,335 + GFF55 0.59 -0.5 70,570 + GFF55 0.88 -0.0 70,570 + GFF55 0.88 -1.4 70,570 + GFF55 0.88 -0.3 70,570 + GFF55 0.88 -1.4 70,570 + GFF55 0.88 -0.7 70,570 + GFF55 0.88 +0.3 70,571 - GFF55 0.88 +0.4 71,327 + GFF56 0.71 +2.0 71,327 + GFF56 0.71 +1.6 71,328 - GFF56 0.71 +2.2 71,328 - GFF56 0.71 +0.2 71,328 - GFF56 0.71 +0.0 71,412 - GFF56 0.80 -1.0 71,616 + -0.2 71,617 - -2.7 71,983 - GFF57 0.27 -1.3 71,983 - GFF57 0.27 +0.0 72,022 - GFF57 0.30 -1.0 72,022 - GFF57 0.30 -0.6 72,022 - GFF57 0.30 -0.4 72,141 + GFF57 0.40 +0.4 72,274 - GFF57 0.51 -0.9 72,525 + GFF57 0.71 +0.1 72,526 - GFF57 0.71 -0.4 72,526 - GFF57 0.71 -0.3
Or see this region's nucleotide sequence