Strain Fitness in Variovorax sp. SCN45 around GFF5460

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
90,163	+		GFF5459	0.27	+0.0
90,164	-		GFF5459	0.27	+1.2
90,164	-		GFF5459	0.27	+0.9
90,206	+		GFF5459	0.30	+0.8
90,206	+		GFF5459	0.30	-1.3
90,207	-		GFF5459	0.31	-1.1
90,207	-		GFF5459	0.31	+1.1
90,207	-		GFF5459	0.31	-0.6
90,207	-		GFF5459	0.31	+0.6
90,336	-		GFF5459	0.41	-0.6
90,753	-		GFF5459	0.73	-1.0
91,075	+				-0.1
91,075	+				-1.1
91,075	+				-0.3
91,075	+				-0.4
91,075	+				-0.4
91,075	+				-0.8
91,075	+				+0.6
91,075	+				-0.6
91,075	+				+1.3
91,075	+				-0.6
91,076	-				-1.1
91,076	-				-0.9
91,076	-				-0.1
91,076	-				-0.1
91,150	+				+0.0
91,151	-				-0.4
91,313	+		GFF5460	0.34	-1.3
91,327	+		GFF5460	0.36	-0.3
91,328	-		GFF5460	0.36	+1.0
91,447	+		GFF5460	0.54	-3.2
91,447	+		GFF5460	0.54	-0.4
91,447	+		GFF5460	0.54	-0.1
91,448	-		GFF5460	0.54	-0.6
91,753	+				+0.0
91,753	+				-0.9
91,754	-				-0.0
91,754	-				-0.5
91,864	+		GFF5461	0.12	+0.1
91,864	+		GFF5461	0.12	-0.5
91,864	+		GFF5461	0.12	-0.3
91,864	+		GFF5461	0.12	-0.0
91,865	-		GFF5461	0.12	-0.3
91,865	-		GFF5461	0.12	+0.1
91,865	-		GFF5461	0.12	-0.2
91,876	+		GFF5461	0.14	-0.5
92,128	+		GFF5461	0.44	+0.5
92,128	+		GFF5461	0.44	-1.1
92,128	+		GFF5461	0.44	+0.3
92,128	+		GFF5461	0.44	+0.6
92,129	-		GFF5461	0.44	-0.9
92,129	-		GFF5461	0.44	-1.6
92,129	-		GFF5461	0.44	-1.0
92,183	+		GFF5461	0.51	-0.5
92,184	-		GFF5461	0.51	+0.1
92,184	-		GFF5461	0.51	-0.3
92,302	+		GFF5461	0.66	-0.5
92,302	+		GFF5461	0.66	-0.3
92,302	+		GFF5461	0.66	+1.2
92,303	-		GFF5461	0.66	+1.2
92,303	-		GFF5461	0.66	-0.8
92,303	-		GFF5461	0.66	-0.8
92,339	-		GFF5461	0.70	-1.0
92,350	+		GFF5461	0.71	+0.7
92,350	+		GFF5461	0.71	-1.9
92,351	-		GFF5461	0.72	+0.6
92,351	-		GFF5461	0.72	+0.6
92,431	+		GFF5461	0.81	-0.4
92,431	+		GFF5461	0.81	-1.3
92,431	+		GFF5461	0.81	-1.6
92,431	+		GFF5461	0.81	-0.6
92,431	+		GFF5461	0.81	-1.8
92,431	+		GFF5461	0.81	+0.2
92,431	+		GFF5461	0.81	-0.2
92,431	+		GFF5461	0.81	-0.7
92,432	-		GFF5461	0.81	+0.4
92,432	-		GFF5461	0.81	-0.5
92,432	-		GFF5461	0.81	-0.3
92,432	-		GFF5461	0.81	+1.2
92,432	-		GFF5461	0.81	+0.1
92,432	-		GFF5461	0.81	-0.1
92,432	-		GFF5461	0.81	+0.2
92,437	+		GFF5461	0.82	+1.9
92,437	+		GFF5461	0.82	-0.5
92,437	+		GFF5461	0.82	+1.2
92,437	+		GFF5461	0.82	-0.9
92,437	+		GFF5461	0.82	-1.0
92,437	+		GFF5461	0.82	-0.3
92,438	-		GFF5461	0.82	+0.6
92,438	-		GFF5461	0.82	-0.1
92,503	+				+1.2
92,503	+				-1.4
92,737	+		GFF5462	0.13	-1.3
92,737	+		GFF5462	0.13	+1.0
92,737	+		GFF5462	0.13	+0.1
92,738	-		GFF5462	0.13	-0.1