Strain Fitness in Variovorax sp. SCN45 around GFF519

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF518 and GFF519 are separated by 145 nucleotidesGFF519 and GFF520 overlap by 4 nucleotides GFF518 - Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains, at 556,972 to 558,504 GFF518 GFF519 - Transcriptional regulator, AcrR family, at 558,650 to 559,345 GFF519 GFF520 - Efflux transport system, outer membrane factor (OMF) lipoprotein, at 559,342 to 560,868 GFF520 Position (kb) 558 559 560Strain fitness (log2 ratio) -2 -1 0 1 2at 557.957 kb on + strand, within GFF518at 557.957 kb on + strand, within GFF518at 557.957 kb on + strand, within GFF518at 557.957 kb on + strand, within GFF518at 557.957 kb on + strand, within GFF518at 557.957 kb on + strand, within GFF518at 557.958 kb on - strand, within GFF518at 557.958 kb on - strand, within GFF518at 557.958 kb on - strand, within GFF518at 557.958 kb on - strand, within GFF518at 557.993 kb on + strand, within GFF518at 557.994 kb on - strand, within GFF518at 558.364 kb on + strandat 558.364 kb on + strandat 558.364 kb on + strandat 558.364 kb on + strandat 558.365 kb on - strandat 558.365 kb on - strandat 558.515 kb on + strandat 558.516 kb on - strandat 558.516 kb on - strandat 558.582 kb on - strandat 558.606 kb on - strandat 558.606 kb on - strandat 558.606 kb on - strandat 558.639 kb on + strandat 558.639 kb on + strandat 558.827 kb on + strand, within GFF519at 558.828 kb on - strand, within GFF519at 558.828 kb on - strand, within GFF519at 558.828 kb on - strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.831 kb on - strand, within GFF519at 558.831 kb on - strand, within GFF519at 558.831 kb on - strand, within GFF519at 558.831 kb on - strand, within GFF519at 558.855 kb on - strand, within GFF519at 558.949 kb on + strand, within GFF519at 558.949 kb on + strand, within GFF519at 558.950 kb on - strand, within GFF519at 558.950 kb on - strand, within GFF519at 558.950 kb on - strand, within GFF519at 558.950 kb on - strand, within GFF519at 558.950 kb on - strand, within GFF519at 559.190 kb on + strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.734 kb on + strand, within GFF520at 559.734 kb on + strand, within GFF520at 559.734 kb on + strand, within GFF520at 559.734 kb on + strand, within GFF520at 559.735 kb on - strand, within GFF520at 559.735 kb on - strand, within GFF520at 559.918 kb on + strand, within GFF520at 559.918 kb on + strand, within GFF520at 560.260 kb on + strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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557,957 + GFF518 0.64 +0.1
557,957 + GFF518 0.64 -1.0
557,957 + GFF518 0.64 -0.9
557,957 + GFF518 0.64 +1.4
557,957 + GFF518 0.64 -0.1
557,957 + GFF518 0.64 -0.0
557,958 - GFF518 0.64 +1.1
557,958 - GFF518 0.64 -0.2
557,958 - GFF518 0.64 -0.1
557,958 - GFF518 0.64 +0.4
557,993 + GFF518 0.67 -0.5
557,994 - GFF518 0.67 -0.6
558,364 + +0.2
558,364 + -0.1
558,364 + -1.8
558,364 + -0.8
558,365 - -0.7
558,365 - -0.3
558,515 + +1.6
558,516 - -0.9
558,516 - -0.8
558,582 - +0.4
558,606 - +0.7
558,606 - +1.6
558,606 - -0.0
558,639 + -1.2
558,639 + +0.1
558,827 + GFF519 0.25 -1.0
558,828 - GFF519 0.26 +0.8
558,828 - GFF519 0.26 -0.3
558,828 - GFF519 0.26 -0.0
558,830 + GFF519 0.26 +0.3
558,830 + GFF519 0.26 +0.7
558,830 + GFF519 0.26 -1.0
558,830 + GFF519 0.26 +0.8
558,830 + GFF519 0.26 +0.0
558,831 - GFF519 0.26 +1.0
558,831 - GFF519 0.26 +0.7
558,831 - GFF519 0.26 +2.0
558,831 - GFF519 0.26 -0.0
558,855 - GFF519 0.29 -1.9
558,949 + GFF519 0.43 -0.4
558,949 + GFF519 0.43 -0.4
558,950 - GFF519 0.43 -1.3
558,950 - GFF519 0.43 +1.3
558,950 - GFF519 0.43 +0.6
558,950 - GFF519 0.43 -0.6
558,950 - GFF519 0.43 +0.0
559,190 + GFF519 0.78 -1.3
559,191 - GFF519 0.78 +0.6
559,191 - GFF519 0.78 -0.9
559,191 - GFF519 0.78 -1.0
559,191 - GFF519 0.78 +0.0
559,191 - GFF519 0.78 -0.6
559,191 - GFF519 0.78 -0.4
559,191 - GFF519 0.78 -0.3
559,734 + GFF520 0.26 -0.3
559,734 + GFF520 0.26 -0.1
559,734 + GFF520 0.26 -0.5
559,734 + GFF520 0.26 -1.9
559,735 - GFF520 0.26 +0.7
559,735 - GFF520 0.26 +0.1
559,918 + GFF520 0.38 -0.4
559,918 + GFF520 0.38 -0.2
560,260 + GFF520 0.60 -0.2
560,261 - GFF520 0.60 -0.6
560,261 - GFF520 0.60 +0.4
560,261 - GFF520 0.60 +1.7
560,261 - GFF520 0.60 -0.7
560,261 - GFF520 0.60 +0.9

Or see this region's nucleotide sequence