Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4915 and GFF4916 are separated by 48 nucleotides GFF4916 and GFF4917 are separated by 13 nucleotides GFF4917 and GFF4918 overlap by 4 nucleotides GFF4918 and GFF4919 are separated by 216 nucleotides
GFF4915 - hypothetical protein, at 7,772 to 9,454
GFF4915
GFF4916 - FIG00959407: hypothetical protein, at 9,503 to 10,102
GFF4916
GFF4917 - no description, at 10,116 to 11,918
GFF4917
GFF4918 - hypothetical protein, at 11,915 to 12,520
GFF4918
GFF4919 - hypothetical protein, at 12,737 to 13,396
GFF4919
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-1
0
1
2 at 9.378 kb on + strand at 9.378 kb on + strand at 9.378 kb on + strand at 9.378 kb on + strand at 9.378 kb on + strand at 9.378 kb on + strand at 9.379 kb on - strand at 9.379 kb on - strand at 9.379 kb on - strand at 9.379 kb on - strand at 9.379 kb on - strand at 9.379 kb on - strand at 9.379 kb on - strand at 9.483 kb on + strand at 9.483 kb on + strand at 9.483 kb on + strand at 9.483 kb on + strand at 9.484 kb on - strand at 9.484 kb on - strand at 9.484 kb on - strand at 9.529 kb on + strand at 9.529 kb on + strand at 9.529 kb on + strand at 9.530 kb on - strand at 9.588 kb on + strand, within GFF4916 at 9.743 kb on + strand, within GFF4916 at 9.744 kb on - strand, within GFF4916 at 9.985 kb on - strand, within GFF4916 at 9.985 kb on - strand, within GFF4916 at 9.985 kb on - strand, within GFF4916 at 11.096 kb on - strand, within GFF4917 at 11.096 kb on - strand, within GFF4917 at 11.096 kb on - strand, within GFF4917 at 11.096 kb on - strand, within GFF4917 at 11.313 kb on + strand, within GFF4917 at 11.313 kb on + strand, within GFF4917 at 11.314 kb on - strand, within GFF4917 at 11.314 kb on - strand, within GFF4917 at 11.314 kb on - strand, within GFF4917 at 11.383 kb on + strand, within GFF4917 at 11.383 kb on + strand, within GFF4917 at 11.383 kb on + strand, within GFF4917 at 11.384 kb on - strand, within GFF4917 at 11.384 kb on - strand, within GFF4917 at 11.384 kb on - strand, within GFF4917 at 11.384 kb on - strand, within GFF4917 at 11.384 kb on - strand, within GFF4917 at 11.384 kb on - strand, within GFF4917 at 11.384 kb on - strand, within GFF4917 at 11.384 kb on - strand, within GFF4917 at 12.065 kb on + strand, within GFF4918 at 12.144 kb on + strand, within GFF4918 at 12.144 kb on + strand, within GFF4918 at 12.144 kb on + strand, within GFF4918 at 12.144 kb on + strand, within GFF4918 at 12.144 kb on + strand, within GFF4918 at 12.144 kb on + strand, within GFF4918 at 12.145 kb on - strand, within GFF4918 at 12.145 kb on - strand, within GFF4918 at 12.145 kb on - strand, within GFF4918 at 12.300 kb on + strand, within GFF4918 at 12.301 kb on - strand, within GFF4918 at 12.301 kb on - strand, within GFF4918 at 12.301 kb on - strand, within GFF4918 at 12.301 kb on - strand, within GFF4918 at 12.301 kb on - strand, within GFF4918 at 12.414 kb on + strand, within GFF4918 at 12.414 kb on + strand, within GFF4918 at 12.414 kb on + strand, within GFF4918 at 12.415 kb on - strand, within GFF4918 at 12.415 kb on - strand, within GFF4918 at 12.415 kb on - strand, within GFF4918 at 12.415 kb on - strand, within GFF4918 at 12.415 kb on - strand, within GFF4918 at 12.453 kb on + strand, within GFF4918 at 12.453 kb on + strand, within GFF4918 at 12.454 kb on - strand, within GFF4918 at 12.454 kb on - strand, within GFF4918 at 12.454 kb on - strand, within GFF4918 at 12.511 kb on + strand at 12.511 kb on + strand at 12.511 kb on + strand at 12.511 kb on + strand at 12.511 kb on + strand at 12.512 kb on - strand at 12.512 kb on - strand at 12.512 kb on - strand at 12.525 kb on - strand at 12.525 kb on - strand at 12.587 kb on + strand at 12.587 kb on + strand at 12.620 kb on - strand at 12.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 9,378 + +0.9 9,378 + +0.7 9,378 + -0.3 9,378 + -0.2 9,378 + +0.9 9,378 + +0.4 9,379 - -0.1 9,379 - -0.9 9,379 - -0.4 9,379 - +0.6 9,379 - -0.6 9,379 - -0.0 9,379 - -0.2 9,483 + -0.4 9,483 + -0.4 9,483 + -0.5 9,483 + -0.1 9,484 - -0.4 9,484 - -0.9 9,484 - -0.8 9,529 + -1.6 9,529 + -1.5 9,529 + -0.1 9,530 - -0.3 9,588 + GFF4916 0.14 -0.5 9,743 + GFF4916 0.40 +0.2 9,744 - GFF4916 0.40 -1.2 9,985 - GFF4916 0.80 -0.6 9,985 - GFF4916 0.80 +0.5 9,985 - GFF4916 0.80 -0.5 11,096 - GFF4917 0.54 +0.3 11,096 - GFF4917 0.54 -0.7 11,096 - GFF4917 0.54 +1.4 11,096 - GFF4917 0.54 -0.6 11,313 + GFF4917 0.66 +0.3 11,313 + GFF4917 0.66 -0.3 11,314 - GFF4917 0.66 -0.7 11,314 - GFF4917 0.66 -0.5 11,314 - GFF4917 0.66 +0.7 11,383 + GFF4917 0.70 +0.7 11,383 + GFF4917 0.70 +1.4 11,383 + GFF4917 0.70 +2.1 11,384 - GFF4917 0.70 +1.5 11,384 - GFF4917 0.70 +0.5 11,384 - GFF4917 0.70 -0.3 11,384 - GFF4917 0.70 -0.1 11,384 - GFF4917 0.70 -0.2 11,384 - GFF4917 0.70 +0.4 11,384 - GFF4917 0.70 +2.1 11,384 - GFF4917 0.70 -0.7 12,065 + GFF4918 0.25 -1.2 12,144 + GFF4918 0.38 -0.8 12,144 + GFF4918 0.38 +0.0 12,144 + GFF4918 0.38 +0.4 12,144 + GFF4918 0.38 -0.1 12,144 + GFF4918 0.38 -0.1 12,144 + GFF4918 0.38 +0.7 12,145 - GFF4918 0.38 -0.1 12,145 - GFF4918 0.38 -0.5 12,145 - GFF4918 0.38 +0.1 12,300 + GFF4918 0.64 -0.5 12,301 - GFF4918 0.64 +0.4 12,301 - GFF4918 0.64 -1.1 12,301 - GFF4918 0.64 -0.5 12,301 - GFF4918 0.64 +1.0 12,301 - GFF4918 0.64 +0.5 12,414 + GFF4918 0.82 -0.1 12,414 + GFF4918 0.82 -0.5 12,414 + GFF4918 0.82 -1.6 12,415 - GFF4918 0.83 -0.1 12,415 - GFF4918 0.83 -0.3 12,415 - GFF4918 0.83 +0.4 12,415 - GFF4918 0.83 +0.0 12,415 - GFF4918 0.83 -1.2 12,453 + GFF4918 0.89 -0.8 12,453 + GFF4918 0.89 -0.1 12,454 - GFF4918 0.89 +0.0 12,454 - GFF4918 0.89 +0.3 12,454 - GFF4918 0.89 -0.2 12,511 + +0.6 12,511 + -1.5 12,511 + +1.9 12,511 + -0.6 12,511 + +0.4 12,512 - -0.3 12,512 - -0.7 12,512 - -0.7 12,525 - +0.2 12,525 - -0.2 12,587 + -0.2 12,587 + -1.2 12,620 - -0.3 12,720 + -0.6
Or see this region's nucleotide sequence