Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4672 and GFF4673 are separated by 34 nucleotides GFF4673 and GFF4674 are separated by 150 nucleotides GFF4674 and GFF4675 are separated by 3 nucleotides GFF4675 and GFF4676 are separated by 11 nucleotides
GFF4672 - Exoenzymes regulatory protein AepA precursor, at 29,215 to 30,900
GFF4672
GFF4673 - hypothetical protein, at 30,935 to 31,699
GFF4673
GFF4674 - Amidohydrolase family protein, at 31,850 to 33,190
GFF4674
GFF4675 - no description, at 33,194 to 33,754
GFF4675
GFF4676 - hypothetical protein, at 33,766 to 34,902
GFF4676
Position (kb)
31
32
33
34 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 30.906 kb on + strand at 30.906 kb on + strand at 30.906 kb on + strand at 30.907 kb on - strand at 30.907 kb on - strand at 30.907 kb on - strand at 30.990 kb on + strand at 30.990 kb on + strand at 30.991 kb on - strand at 30.991 kb on - strand at 30.991 kb on - strand at 31.005 kb on + strand at 31.005 kb on + strand at 31.005 kb on + strand at 31.005 kb on + strand at 31.005 kb on + strand at 31.006 kb on - strand at 31.006 kb on - strand at 31.006 kb on - strand at 31.006 kb on - strand at 31.006 kb on - strand at 31.163 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.276 kb on - strand, within GFF4673 at 31.276 kb on - strand, within GFF4673 at 31.276 kb on - strand, within GFF4673 at 31.467 kb on + strand, within GFF4673 at 31.467 kb on + strand, within GFF4673 at 31.467 kb on + strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.564 kb on - strand, within GFF4673 at 31.564 kb on - strand, within GFF4673 at 31.564 kb on - strand, within GFF4673 at 31.572 kb on + strand, within GFF4673 at 31.573 kb on - strand, within GFF4673 at 31.573 kb on - strand, within GFF4673 at 31.573 kb on - strand, within GFF4673 at 31.573 kb on - strand, within GFF4673 at 31.720 kb on + strand at 31.720 kb on + strand at 31.720 kb on + strand at 31.721 kb on - strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.793 kb on + strand at 31.793 kb on + strand at 31.793 kb on + strand at 31.794 kb on - strand at 31.794 kb on - strand at 32.033 kb on + strand, within GFF4674 at 32.033 kb on + strand, within GFF4674 at 32.034 kb on - strand, within GFF4674 at 32.034 kb on - strand, within GFF4674 at 32.034 kb on - strand, within GFF4674 at 32.237 kb on + strand, within GFF4674 at 32.237 kb on + strand, within GFF4674 at 32.237 kb on + strand, within GFF4674 at 32.237 kb on + strand, within GFF4674 at 32.237 kb on + strand, within GFF4674 at 32.238 kb on - strand, within GFF4674 at 32.783 kb on + strand, within GFF4674 at 32.784 kb on - strand, within GFF4674 at 32.784 kb on - strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.889 kb on - strand, within GFF4674 at 33.011 kb on + strand, within GFF4674 at 33.011 kb on + strand, within GFF4674 at 33.011 kb on + strand, within GFF4674 at 33.012 kb on - strand, within GFF4674 at 33.012 kb on - strand, within GFF4674 at 33.107 kb on + strand at 33.107 kb on + strand at 33.107 kb on + strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.168 kb on - strand at 33.168 kb on - strand at 33.168 kb on - strand at 33.168 kb on - strand at 33.168 kb on - strand at 33.188 kb on + strand at 33.188 kb on + strand at 33.188 kb on + strand at 33.188 kb on + strand at 33.189 kb on - strand at 33.189 kb on - strand at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.298 kb on - strand, within GFF4675 at 33.298 kb on - strand, within GFF4675 at 33.298 kb on - strand, within GFF4675 at 33.313 kb on + strand, within GFF4675 at 33.313 kb on + strand, within GFF4675 at 33.314 kb on - strand, within GFF4675 at 33.404 kb on + strand, within GFF4675 at 33.404 kb on + strand, within GFF4675 at 33.405 kb on - strand, within GFF4675 at 33.405 kb on - strand, within GFF4675 at 33.552 kb on + strand, within GFF4675 at 33.553 kb on - strand, within GFF4675 at 33.575 kb on - strand, within GFF4675 at 33.638 kb on + strand, within GFF4675 at 33.639 kb on - strand, within GFF4675 at 33.643 kb on + strand, within GFF4675 at 33.643 kb on + strand, within GFF4675 at 33.643 kb on + strand, within GFF4675 at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.828 kb on + strand at 33.828 kb on + strand at 33.829 kb on - strand at 33.829 kb on - strand at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 34.040 kb on + strand, within GFF4676 at 34.040 kb on + strand, within GFF4676 at 34.041 kb on - strand, within GFF4676 at 34.041 kb on - strand, within GFF4676 at 34.041 kb on - strand, within GFF4676 at 34.151 kb on + strand, within GFF4676 at 34.151 kb on + strand, within GFF4676 at 34.152 kb on - strand, within GFF4676 at 34.152 kb on - strand, within GFF4676 at 34.152 kb on - strand, within GFF4676
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 30,906 + +0.2 30,906 + +0.7 30,906 + +0.7 30,907 - -0.9 30,907 - +0.2 30,907 - +0.9 30,990 + -0.2 30,990 + +0.1 30,991 - -0.0 30,991 - +0.4 30,991 - -1.5 31,005 + -0.5 31,005 + +0.1 31,005 + -0.4 31,005 + -0.5 31,005 + -1.0 31,006 - -1.3 31,006 - +0.2 31,006 - -0.9 31,006 - -0.3 31,006 - -1.0 31,163 + GFF4673 0.30 -0.9 31,263 + GFF4673 0.43 +0.4 31,263 + GFF4673 0.43 -0.4 31,263 + GFF4673 0.43 -0.1 31,263 + GFF4673 0.43 -0.5 31,263 + GFF4673 0.43 -0.3 31,263 + GFF4673 0.43 +0.1 31,264 - GFF4673 0.43 -0.3 31,264 - GFF4673 0.43 -1.6 31,264 - GFF4673 0.43 +0.7 31,264 - GFF4673 0.43 -0.8 31,264 - GFF4673 0.43 -0.7 31,264 - GFF4673 0.43 +0.7 31,276 - GFF4673 0.45 -0.6 31,276 - GFF4673 0.45 -0.6 31,276 - GFF4673 0.45 +0.1 31,467 + GFF4673 0.70 +0.2 31,467 + GFF4673 0.70 +0.7 31,467 + GFF4673 0.70 -0.3 31,468 - GFF4673 0.70 +0.2 31,468 - GFF4673 0.70 -1.0 31,468 - GFF4673 0.70 -0.3 31,468 - GFF4673 0.70 -0.1 31,468 - GFF4673 0.70 +0.5 31,563 + GFF4673 0.82 +0.1 31,563 + GFF4673 0.82 -1.0 31,563 + GFF4673 0.82 -0.2 31,563 + GFF4673 0.82 +0.2 31,563 + GFF4673 0.82 +0.1 31,563 + GFF4673 0.82 -0.5 31,563 + GFF4673 0.82 +0.1 31,564 - GFF4673 0.82 +0.1 31,564 - GFF4673 0.82 -0.3 31,564 - GFF4673 0.82 -1.5 31,572 + GFF4673 0.83 -0.5 31,573 - GFF4673 0.83 -0.7 31,573 - GFF4673 0.83 -0.5 31,573 - GFF4673 0.83 -0.2 31,573 - GFF4673 0.83 -0.4 31,720 + -0.7 31,720 + -0.2 31,720 + +0.1 31,721 - -1.3 31,772 + -1.1 31,772 + +0.7 31,772 + +0.1 31,772 + -0.1 31,772 + -0.0 31,772 + -0.3 31,772 + +0.2 31,772 + +0.2 31,772 + -0.3 31,772 + -0.3 31,772 + +1.4 31,773 - +1.3 31,773 - -0.1 31,773 - -0.8 31,773 - -0.9 31,773 - +0.4 31,773 - -0.3 31,773 - +0.2 31,773 - -1.2 31,773 - +0.7 31,773 - -0.9 31,773 - -0.1 31,773 - -2.7 31,773 - -0.2 31,773 - +0.8 31,773 - +1.1 31,773 - -0.3 31,773 - -1.3 31,773 - +0.5 31,793 + -0.1 31,793 + -1.0 31,793 + +0.5 31,794 - +0.0 31,794 - +0.7 32,033 + GFF4674 0.14 +0.5 32,033 + GFF4674 0.14 +0.1 32,034 - GFF4674 0.14 -0.4 32,034 - GFF4674 0.14 -1.3 32,034 - GFF4674 0.14 +0.2 32,237 + GFF4674 0.29 -0.3 32,237 + GFF4674 0.29 -1.6 32,237 + GFF4674 0.29 +0.5 32,237 + GFF4674 0.29 -1.6 32,237 + GFF4674 0.29 -0.8 32,238 - GFF4674 0.29 -1.0 32,783 + GFF4674 0.70 -2.4 32,784 - GFF4674 0.70 -1.1 32,784 - GFF4674 0.70 -1.2 32,888 + GFF4674 0.77 -0.4 32,888 + GFF4674 0.77 -0.5 32,888 + GFF4674 0.77 +0.1 32,888 + GFF4674 0.77 -0.3 32,888 + GFF4674 0.77 +0.2 32,888 + GFF4674 0.77 +0.1 32,888 + GFF4674 0.77 +0.4 32,888 + GFF4674 0.77 +1.4 32,889 - GFF4674 0.77 -0.8 33,011 + GFF4674 0.87 +0.7 33,011 + GFF4674 0.87 -0.2 33,011 + GFF4674 0.87 -0.4 33,012 - GFF4674 0.87 +1.9 33,012 - GFF4674 0.87 +0.1 33,107 + -0.9 33,107 + -0.1 33,107 + -0.4 33,108 - +0.0 33,108 - +0.1 33,108 - -1.4 33,108 - -1.1 33,108 - +0.3 33,108 - -0.5 33,108 - +0.7 33,108 - +1.7 33,108 - -0.7 33,167 + -0.2 33,167 + -1.3 33,167 + -1.3 33,167 + -0.5 33,167 + -0.3 33,167 + -0.5 33,168 - +0.6 33,168 - +0.3 33,168 - -1.3 33,168 - -0.4 33,168 - +1.7 33,188 + +1.9 33,188 + +0.1 33,188 + -2.1 33,188 + -1.8 33,189 - +2.5 33,189 - -0.3 33,297 + GFF4675 0.18 -0.7 33,297 + GFF4675 0.18 -2.7 33,297 + GFF4675 0.18 +0.4 33,297 + GFF4675 0.18 +0.0 33,297 + GFF4675 0.18 -0.6 33,297 + GFF4675 0.18 -1.0 33,297 + GFF4675 0.18 -3.9 33,297 + GFF4675 0.18 +0.1 33,297 + GFF4675 0.18 -0.3 33,298 - GFF4675 0.19 -0.1 33,298 - GFF4675 0.19 +0.2 33,298 - GFF4675 0.19 -1.1 33,313 + GFF4675 0.21 -1.9 33,313 + GFF4675 0.21 -1.0 33,314 - GFF4675 0.21 -0.9 33,404 + GFF4675 0.37 -0.5 33,404 + GFF4675 0.37 -2.1 33,405 - GFF4675 0.38 +0.6 33,405 - GFF4675 0.38 -0.6 33,552 + GFF4675 0.64 -1.2 33,553 - GFF4675 0.64 -0.2 33,575 - GFF4675 0.68 -0.1 33,638 + GFF4675 0.79 -1.5 33,639 - GFF4675 0.79 -1.8 33,643 + GFF4675 0.80 -0.1 33,643 + GFF4675 0.80 +0.1 33,643 + GFF4675 0.80 -0.4 33,767 + +0.1 33,767 + -0.4 33,767 + -0.9 33,767 + -1.0 33,767 + -0.4 33,767 + -0.0 33,767 + -0.2 33,767 + -0.8 33,767 + +2.0 33,767 + +0.8 33,767 + -0.2 33,767 + -0.1 33,767 + -0.1 33,767 + -0.3 33,767 + -1.8 33,767 + +1.4 33,767 + -0.6 33,767 + -0.3 33,767 + -2.8 33,768 - +0.1 33,768 - -0.1 33,768 - +0.7 33,768 - +0.7 33,768 - -0.0 33,768 - +0.2 33,768 - +0.3 33,768 - +0.8 33,768 - -0.8 33,768 - -0.6 33,768 - -0.8 33,828 + +0.6 33,828 + -0.5 33,829 - -0.9 33,829 - -0.6 33,933 + GFF4676 0.15 -0.4 33,933 + GFF4676 0.15 +0.7 33,933 + GFF4676 0.15 -0.0 33,933 + GFF4676 0.15 -1.1 33,933 + GFF4676 0.15 -0.3 33,933 + GFF4676 0.15 +0.2 33,934 - GFF4676 0.15 +0.9 33,934 - GFF4676 0.15 +0.4 33,934 - GFF4676 0.15 +0.7 33,934 - GFF4676 0.15 +1.0 33,934 - GFF4676 0.15 -0.5 33,934 - GFF4676 0.15 -2.5 33,934 - GFF4676 0.15 +0.1 34,040 + GFF4676 0.24 -0.0 34,040 + GFF4676 0.24 +0.7 34,041 - GFF4676 0.24 +0.0 34,041 - GFF4676 0.24 -0.2 34,041 - GFF4676 0.24 -1.8 34,151 + GFF4676 0.34 +0.7 34,151 + GFF4676 0.34 -0.9 34,152 - GFF4676 0.34 +1.3 34,152 - GFF4676 0.34 -2.0 34,152 - GFF4676 0.34 +0.4
Or see this region's nucleotide sequence