Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4530 and GFF4531 overlap by 4 nucleotides GFF4531 and GFF4532 are separated by 140 nucleotides GFF4532 and GFF4533 overlap by 11 nucleotides
GFF4530 - MBL-fold metallo-hydrolase superfamily, at 168,149 to 169,825
GFF4530
GFF4531 - Pseudouridine synthase, at 169,822 to 170,547
GFF4531
GFF4532 - hypothetical protein, at 170,688 to 171,233
GFF4532
GFF4533 - Multidrug resistance transporter, Bcr/CflA family, at 171,223 to 172,452
GFF4533
Position (kb) 170
171
172 Strain fitness (log2 ratio) -2
-1
0
1 at 169.733 kb on + strand at 169.827 kb on + strand at 169.827 kb on + strand at 169.828 kb on - strand at 169.828 kb on - strand at 169.828 kb on - strand at 169.925 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.057 kb on - strand, within GFF4531 at 170.216 kb on - strand, within GFF4531 at 170.216 kb on - strand, within GFF4531 at 170.521 kb on + strand at 170.522 kb on - strand at 170.522 kb on - strand at 170.522 kb on - strand at 170.522 kb on - strand at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.758 kb on - strand, within GFF4532 at 170.758 kb on - strand, within GFF4532 at 171.106 kb on - strand, within GFF4532 at 171.147 kb on + strand, within GFF4532 at 171.147 kb on + strand, within GFF4532 at 171.147 kb on + strand, within GFF4532 at 171.147 kb on + strand, within GFF4532 at 171.148 kb on - strand, within GFF4532 at 171.405 kb on - strand, within GFF4533 at 171.683 kb on + strand, within GFF4533 at 171.683 kb on + strand, within GFF4533 at 171.683 kb on + strand, within GFF4533 at 171.684 kb on - strand, within GFF4533 at 171.684 kb on - strand, within GFF4533 at 171.684 kb on - strand, within GFF4533 at 171.684 kb on - strand, within GFF4533 at 171.684 kb on - strand, within GFF4533 at 171.684 kb on - strand, within GFF4533 at 171.755 kb on + strand, within GFF4533 at 171.755 kb on + strand, within GFF4533 at 171.755 kb on + strand, within GFF4533 at 171.755 kb on + strand, within GFF4533 at 171.755 kb on + strand, within GFF4533 at 171.755 kb on + strand, within GFF4533 at 171.755 kb on + strand, within GFF4533 at 171.755 kb on + strand, within GFF4533 at 171.756 kb on - strand, within GFF4533 at 171.756 kb on - strand, within GFF4533 at 171.756 kb on - strand, within GFF4533 at 171.756 kb on - strand, within GFF4533 at 171.756 kb on - strand, within GFF4533 at 171.756 kb on - strand, within GFF4533 at 171.756 kb on - strand, within GFF4533 at 171.756 kb on - strand, within GFF4533 at 171.827 kb on + strand, within GFF4533 at 171.827 kb on + strand, within GFF4533 at 171.828 kb on - strand, within GFF4533 at 171.935 kb on + strand, within GFF4533 at 171.935 kb on + strand, within GFF4533 at 171.936 kb on - strand, within GFF4533 at 171.936 kb on - strand, within GFF4533 at 171.936 kb on - strand, within GFF4533 at 171.936 kb on - strand, within GFF4533 at 171.936 kb on - strand, within GFF4533 at 172.184 kb on + strand, within GFF4533 at 172.184 kb on + strand, within GFF4533 at 172.185 kb on - strand, within GFF4533 at 172.185 kb on - strand, within GFF4533 at 172.185 kb on - strand, within GFF4533 at 172.185 kb on - strand, within GFF4533 at 172.185 kb on - strand, within GFF4533 at 172.185 kb on - strand, within GFF4533 at 172.185 kb on - strand, within GFF4533 at 172.185 kb on - strand, within GFF4533
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 169,733 + -2.3 169,827 + -1.5 169,827 + -0.2 169,828 - +0.5 169,828 - +0.4 169,828 - +0.0 169,925 + GFF4531 0.14 +0.2 170,056 + GFF4531 0.32 +1.0 170,056 + GFF4531 0.32 -0.6 170,056 + GFF4531 0.32 -1.2 170,056 + GFF4531 0.32 +0.3 170,056 + GFF4531 0.32 +0.1 170,056 + GFF4531 0.32 -0.6 170,057 - GFF4531 0.32 -0.3 170,216 - GFF4531 0.54 -0.3 170,216 - GFF4531 0.54 -0.1 170,521 + -1.3 170,522 - -0.7 170,522 - +0.2 170,522 - -0.4 170,522 - +0.5 170,757 + GFF4532 0.13 -0.9 170,757 + GFF4532 0.13 +0.7 170,757 + GFF4532 0.13 -0.7 170,757 + GFF4532 0.13 +0.4 170,757 + GFF4532 0.13 +0.5 170,757 + GFF4532 0.13 -1.0 170,758 - GFF4532 0.13 +0.2 170,758 - GFF4532 0.13 +0.5 171,106 - GFF4532 0.77 +0.7 171,147 + GFF4532 0.84 +0.6 171,147 + GFF4532 0.84 -0.8 171,147 + GFF4532 0.84 -0.5 171,147 + GFF4532 0.84 +0.8 171,148 - GFF4532 0.84 -0.5 171,405 - GFF4533 0.15 -1.1 171,683 + GFF4533 0.37 +0.5 171,683 + GFF4533 0.37 -0.4 171,683 + GFF4533 0.37 +0.2 171,684 - GFF4533 0.37 -0.9 171,684 - GFF4533 0.37 +0.2 171,684 - GFF4533 0.37 +0.2 171,684 - GFF4533 0.37 +0.8 171,684 - GFF4533 0.37 +0.3 171,684 - GFF4533 0.37 +1.0 171,755 + GFF4533 0.43 +0.4 171,755 + GFF4533 0.43 +0.5 171,755 + GFF4533 0.43 -0.3 171,755 + GFF4533 0.43 -1.0 171,755 + GFF4533 0.43 -0.9 171,755 + GFF4533 0.43 -0.4 171,755 + GFF4533 0.43 -0.5 171,755 + GFF4533 0.43 -0.7 171,756 - GFF4533 0.43 +0.4 171,756 - GFF4533 0.43 -0.8 171,756 - GFF4533 0.43 -1.0 171,756 - GFF4533 0.43 -1.3 171,756 - GFF4533 0.43 -0.1 171,756 - GFF4533 0.43 +0.7 171,756 - GFF4533 0.43 -0.3 171,756 - GFF4533 0.43 -0.1 171,827 + GFF4533 0.49 -0.4 171,827 + GFF4533 0.49 +0.2 171,828 - GFF4533 0.49 -0.2 171,935 + GFF4533 0.58 -0.3 171,935 + GFF4533 0.58 -0.5 171,936 - GFF4533 0.58 +0.2 171,936 - GFF4533 0.58 -0.3 171,936 - GFF4533 0.58 -0.1 171,936 - GFF4533 0.58 -0.4 171,936 - GFF4533 0.58 +1.0 172,184 + GFF4533 0.78 +0.8 172,184 + GFF4533 0.78 +0.6 172,185 - GFF4533 0.78 +0.1 172,185 - GFF4533 0.78 -0.3 172,185 - GFF4533 0.78 -0.2 172,185 - GFF4533 0.78 -2.4 172,185 - GFF4533 0.78 -0.0 172,185 - GFF4533 0.78 -0.3 172,185 - GFF4533 0.78 -1.3 172,185 - GFF4533 0.78 +0.9
Or see this region's nucleotide sequence