Strain Fitness in Variovorax sp. SCN45 around GFF4387

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
14,970	+		GFF4385	0.52	+0.0
14,970	+		GFF4385	0.52	-0.5
14,970	+		GFF4385	0.52	+1.2
14,970	+		GFF4385	0.52	-0.4
14,970	+		GFF4385	0.52	-1.6
14,970	+		GFF4385	0.52	-0.4
14,970	+		GFF4385	0.52	-0.3
14,977	+		GFF4385	0.53	-2.3
14,977	+		GFF4385	0.53	-0.4
14,977	+		GFF4385	0.53	-0.0
14,977	+		GFF4385	0.53	-0.3
15,078	+		GFF4385	0.69	-0.9
15,078	+		GFF4385	0.69	-0.2
15,078	+		GFF4385	0.69	+0.1
15,078	+		GFF4385	0.69	+0.1
15,079	-		GFF4385	0.69	-0.6
15,079	-		GFF4385	0.69	+0.4
15,079	-		GFF4385	0.69	+0.2
15,079	-		GFF4385	0.69	-0.5
15,079	-		GFF4385	0.69	-0.2
15,079	-		GFF4385	0.69	-0.4
15,311	+		GFF4386	0.13	+0.7
15,311	+		GFF4386	0.13	+0.3
15,311	+		GFF4386	0.13	+0.3
15,312	-		GFF4386	0.14	-0.5
15,312	-		GFF4386	0.14	-2.0
15,408	+		GFF4386	0.47	+0.8
15,408	+		GFF4386	0.47	-0.1
15,408	+		GFF4386	0.47	-1.5
15,408	+		GFF4386	0.47	+1.5
15,408	+		GFF4386	0.47	-0.4
15,408	+		GFF4386	0.47	-0.5
15,409	-		GFF4386	0.48	+0.1
15,409	-		GFF4386	0.48	-0.4
15,606	+				-0.6
15,606	+				+0.1
15,606	+				+1.8
15,606	+				-0.8
15,606	+				-1.4
15,606	+				-0.3
15,606	+				-0.5
15,606	+				+0.4
15,606	+				+0.6
15,606	+				-1.3
15,606	+				+1.2
15,606	+				-0.4
15,606	+				-0.7
15,606	+				-0.9
15,607	-				-1.1
15,607	-				+0.1
15,607	-				-0.7
15,607	-				-0.7
15,607	-				+0.2
15,607	-				+0.3
15,607	-				+0.6
15,607	-				+1.7
15,607	-				+0.5
15,619	-		GFF4387	0.12	-0.1
15,692	+		GFF4387	0.25	+0.0
15,692	+		GFF4387	0.25	-0.1
15,692	+		GFF4387	0.25	-1.0
15,692	+		GFF4387	0.25	+0.2
15,692	+		GFF4387	0.25	-0.2
15,693	-		GFF4387	0.25	-0.5
15,693	-		GFF4387	0.25	+0.9
15,693	-		GFF4387	0.25	-1.0
15,693	-		GFF4387	0.25	+0.8
15,693	-		GFF4387	0.25	-0.0
15,693	-		GFF4387	0.25	-0.0
15,693	-		GFF4387	0.25	-0.3
15,804	-		GFF4387	0.46	+1.5
15,998	+		GFF4387	0.81	-0.5
15,998	+		GFF4387	0.81	+0.0
15,999	-		GFF4387	0.82	+0.4
16,049	+				+0.2
16,049	+				-0.3
16,049	+				-1.8
16,049	+				-1.7
16,049	+				+0.3
16,049	+				-0.8
16,049	+				-0.5
16,049	+				+0.1
16,050	-				-1.1
16,050	-				+0.2
16,050	-				-0.6
16,050	-				+0.4
16,250	-		GFF4388	0.26	-1.4
16,250	-		GFF4388	0.26	+0.5
16,250	-		GFF4388	0.26	+0.2
16,250	-		GFF4388	0.26	+0.1
16,250	-		GFF4388	0.26	-1.1
16,250	-		GFF4388	0.26	-0.2
16,250	-		GFF4388	0.26	+0.1
16,250	-		GFF4388	0.26	-0.3
16,250	-		GFF4388	0.26	+2.5
16,250	-		GFF4388	0.26	-0.5
16,250	-		GFF4388	0.26	-0.5
16,250	-		GFF4388	0.26	+1.0
16,250	-		GFF4388	0.26	-0.5
16,250	-		GFF4388	0.26	-0.5
16,400	-		GFF4388	0.51	-0.1
16,400	-		GFF4388	0.51	-1.3
16,400	-		GFF4388	0.51	-0.9
16,400	-		GFF4388	0.51	+0.1
16,400	-		GFF4388	0.51	-0.3
16,478	+		GFF4388	0.64	-1.4
16,478	+		GFF4388	0.64	-0.4
16,478	+		GFF4388	0.64	+0.5
16,478	+		GFF4388	0.64	+1.2
16,478	+		GFF4388	0.64	+1.0
16,478	+		GFF4388	0.64	-0.2
16,479	-		GFF4388	0.64	+0.5
16,479	-		GFF4388	0.64	+0.5
16,479	-		GFF4388	0.64	+1.3
16,479	-		GFF4388	0.64	-0.2
16,479	-		GFF4388	0.64	-0.2
16,516	-		GFF4388	0.70	-0.5
16,726	+				-0.1
16,726	+				-0.4
16,726	+				-1.7
16,726	+				-0.0
16,726	+				-0.3
16,726	+				+0.5
16,727	-				-0.5
16,727	-				+0.0
16,727	-				-0.3
16,727	-				-0.5
16,727	-				-0.1
16,727	-				-0.5
16,859	+				+1.1
16,859	+				-0.6
16,859	+				-1.8
16,859	+				-0.5
16,859	+				-0.5
16,859	+				-0.6
16,860	-				+0.3
16,860	-				-1.2
16,860	-				+0.7
17,049	+				+0.5
17,049	+				+0.5
17,050	-				-0.8
17,050	-				+0.2