Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4324 and GFF4325 overlap by 4 nucleotides GFF4325 and GFF4326 are separated by 82 nucleotides GFF4326 and GFF4327 are separated by 43 nucleotides GFF4327 and GFF4328 are separated by 47 nucleotides
GFF4324 - no description, at 246,247 to 247,281
GFF4324
GFF4325 - hypothetical protein, at 247,278 to 247,781
GFF4325
GFF4326 - Branched-chain amino acid ABC transporter, substrate-binding protein LivJ (TC 3.A.1.4.1), at 247,864 to 249,090
GFF4326
GFF4327 - Enoyl-CoA hydratase (EC 4.2.1.17), at 249,134 to 249,931
GFF4327
GFF4328 - hypothetical protein, at 249,979 to 251,094
GFF4328
Position (kb) 247
248
249
250 Strain fitness (log2 ratio) -2
-1
0
1 at 247.015 kb on + strand, within GFF4324 at 247.015 kb on + strand, within GFF4324 at 247.016 kb on - strand, within GFF4324 at 247.066 kb on + strand, within GFF4324 at 247.066 kb on + strand, within GFF4324 at 248.023 kb on + strand, within GFF4326 at 248.024 kb on - strand, within GFF4326 at 248.347 kb on + strand, within GFF4326 at 248.348 kb on - strand, within GFF4326 at 248.348 kb on - strand, within GFF4326 at 248.527 kb on + strand, within GFF4326 at 248.527 kb on + strand, within GFF4326 at 248.528 kb on - strand, within GFF4326 at 250.010 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 247,015 + GFF4324 0.74 -2.3 247,015 + GFF4324 0.74 +0.3 247,016 - GFF4324 0.74 +0.9 247,066 + GFF4324 0.79 -0.2 247,066 + GFF4324 0.79 +0.1 248,023 + GFF4326 0.13 -0.5 248,024 - GFF4326 0.13 +0.7 248,347 + GFF4326 0.39 +0.6 248,348 - GFF4326 0.39 +0.7 248,348 - GFF4326 0.39 -0.5 248,527 + GFF4326 0.54 -0.0 248,527 + GFF4326 0.54 -0.5 248,528 - GFF4326 0.54 +0.0 250,010 + -1.1
Or see this region's nucleotide sequence