Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3930 and GFF3931 are separated by 77 nucleotides GFF3931 and GFF3932 are separated by 1 nucleotides GFF3932 and GFF3933 are separated by 142 nucleotides
GFF3930 - Peptidoglycan lipid II flippase MurJ, at 134,166 to 135,833
GFF3930
GFF3931 - Protein sirB1, at 135,911 to 136,762
GFF3931
GFF3932 - Acetyltransferase, GNAT family, at 136,764 to 137,192
GFF3932
GFF3933 - PhnB protein, at 137,335 to 137,766
GFF3933
Position (kb) 135
136
137 Strain fitness (log2 ratio) -2
-1
0
1
2 at 135.900 kb on + strand at 135.900 kb on + strand at 135.900 kb on + strand at 135.901 kb on - strand at 135.901 kb on - strand at 135.912 kb on - strand at 135.935 kb on - strand at 135.935 kb on - strand at 135.947 kb on + strand at 135.947 kb on + strand at 135.948 kb on - strand at 135.948 kb on - strand at 136.022 kb on + strand, within GFF3931 at 136.022 kb on + strand, within GFF3931 at 136.022 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.203 kb on - strand, within GFF3931 at 136.203 kb on - strand, within GFF3931 at 136.418 kb on + strand, within GFF3931 at 136.418 kb on + strand, within GFF3931 at 136.418 kb on + strand, within GFF3931 at 136.418 kb on + strand, within GFF3931 at 136.419 kb on - strand, within GFF3931 at 136.419 kb on - strand, within GFF3931 at 136.419 kb on - strand, within GFF3931 at 136.469 kb on + strand, within GFF3931 at 136.469 kb on + strand, within GFF3931 at 136.469 kb on + strand, within GFF3931 at 136.470 kb on - strand, within GFF3931 at 136.470 kb on - strand, within GFF3931 at 136.470 kb on - strand, within GFF3931 at 136.470 kb on - strand, within GFF3931 at 136.523 kb on - strand, within GFF3931 at 136.682 kb on + strand at 136.682 kb on + strand at 136.682 kb on + strand at 136.683 kb on - strand at 136.683 kb on - strand at 136.683 kb on - strand at 136.683 kb on - strand at 136.929 kb on + strand, within GFF3932 at 136.929 kb on + strand, within GFF3932 at 136.929 kb on + strand, within GFF3932 at 136.929 kb on + strand, within GFF3932 at 136.930 kb on - strand, within GFF3932 at 136.930 kb on - strand, within GFF3932 at 136.930 kb on - strand, within GFF3932 at 136.960 kb on - strand, within GFF3932 at 136.960 kb on - strand, within GFF3932 at 136.960 kb on - strand, within GFF3932 at 136.974 kb on + strand, within GFF3932 at 136.975 kb on - strand, within GFF3932 at 136.975 kb on - strand, within GFF3932 at 137.073 kb on + strand, within GFF3932 at 137.073 kb on + strand, within GFF3932 at 137.074 kb on - strand, within GFF3932 at 137.074 kb on - strand, within GFF3932 at 137.181 kb on + strand at 137.181 kb on + strand at 137.182 kb on - strand at 137.224 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 135,900 + -1.9 135,900 + -0.1 135,900 + -0.4 135,901 - +0.3 135,901 - -1.1 135,912 - -0.5 135,935 - +0.2 135,935 - -0.1 135,947 + -0.2 135,947 + -0.9 135,948 - -0.3 135,948 - +0.3 136,022 + GFF3931 0.13 -0.2 136,022 + GFF3931 0.13 -0.0 136,022 + GFF3931 0.13 -0.7 136,181 + GFF3931 0.32 -0.6 136,181 + GFF3931 0.32 -0.7 136,181 + GFF3931 0.32 +2.7 136,181 + GFF3931 0.32 +0.5 136,181 + GFF3931 0.32 +1.1 136,181 + GFF3931 0.32 +0.6 136,181 + GFF3931 0.32 +0.3 136,182 - GFF3931 0.32 +0.9 136,182 - GFF3931 0.32 +0.3 136,182 - GFF3931 0.32 +0.7 136,182 - GFF3931 0.32 +0.0 136,182 - GFF3931 0.32 +2.3 136,182 - GFF3931 0.32 +0.9 136,182 - GFF3931 0.32 -0.3 136,184 + GFF3931 0.32 -0.3 136,184 + GFF3931 0.32 -1.0 136,184 + GFF3931 0.32 +0.0 136,184 + GFF3931 0.32 +1.6 136,184 + GFF3931 0.32 -0.5 136,184 + GFF3931 0.32 -0.4 136,184 + GFF3931 0.32 -0.0 136,184 + GFF3931 0.32 +0.2 136,184 + GFF3931 0.32 +0.6 136,184 + GFF3931 0.32 -0.6 136,184 + GFF3931 0.32 -0.7 136,184 + GFF3931 0.32 +0.2 136,184 + GFF3931 0.32 +1.9 136,185 - GFF3931 0.32 -0.2 136,185 - GFF3931 0.32 +0.8 136,185 - GFF3931 0.32 +0.5 136,185 - GFF3931 0.32 +0.5 136,185 - GFF3931 0.32 -0.5 136,185 - GFF3931 0.32 -1.3 136,185 - GFF3931 0.32 -1.2 136,185 - GFF3931 0.32 +1.3 136,185 - GFF3931 0.32 -0.4 136,185 - GFF3931 0.32 -0.2 136,185 - GFF3931 0.32 +0.7 136,185 - GFF3931 0.32 +1.7 136,185 - GFF3931 0.32 -0.4 136,185 - GFF3931 0.32 +1.7 136,185 - GFF3931 0.32 -0.3 136,185 - GFF3931 0.32 +1.9 136,185 - GFF3931 0.32 -0.4 136,202 + GFF3931 0.34 -0.6 136,202 + GFF3931 0.34 +0.1 136,202 + GFF3931 0.34 +0.9 136,202 + GFF3931 0.34 -0.3 136,202 + GFF3931 0.34 +1.4 136,203 - GFF3931 0.34 -1.5 136,203 - GFF3931 0.34 -0.6 136,418 + GFF3931 0.60 +0.4 136,418 + GFF3931 0.60 +0.7 136,418 + GFF3931 0.60 -0.4 136,418 + GFF3931 0.60 -1.0 136,419 - GFF3931 0.60 +0.2 136,419 - GFF3931 0.60 +1.7 136,419 - GFF3931 0.60 -0.1 136,469 + GFF3931 0.65 +1.6 136,469 + GFF3931 0.65 -0.6 136,469 + GFF3931 0.65 -0.4 136,470 - GFF3931 0.66 +0.2 136,470 - GFF3931 0.66 -0.2 136,470 - GFF3931 0.66 +0.3 136,470 - GFF3931 0.66 -1.9 136,523 - GFF3931 0.72 -1.0 136,682 + +0.8 136,682 + -0.1 136,682 + +0.4 136,683 - -0.6 136,683 - -0.2 136,683 - -0.5 136,683 - -0.1 136,929 + GFF3932 0.38 -0.1 136,929 + GFF3932 0.38 +1.6 136,929 + GFF3932 0.38 +0.7 136,929 + GFF3932 0.38 -0.5 136,930 - GFF3932 0.39 -0.4 136,930 - GFF3932 0.39 +2.3 136,930 - GFF3932 0.39 +0.3 136,960 - GFF3932 0.46 +0.3 136,960 - GFF3932 0.46 +0.1 136,960 - GFF3932 0.46 -0.7 136,974 + GFF3932 0.49 -0.5 136,975 - GFF3932 0.49 +1.3 136,975 - GFF3932 0.49 -1.1 137,073 + GFF3932 0.72 -0.6 137,073 + GFF3932 0.72 +0.1 137,074 - GFF3932 0.72 -0.3 137,074 - GFF3932 0.72 +0.2 137,181 + -0.5 137,181 + -0.1 137,182 - -0.5 137,224 + +0.7
Or see this region's nucleotide sequence