Strain Fitness in Variovorax sp. SCN45 around GFF3689

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3687 and GFF3688 are separated by 12 nucleotidesGFF3688 and GFF3689 are separated by 204 nucleotidesGFF3689 and GFF3690 overlap by 4 nucleotides GFF3687 - ATP synthase beta chain (EC 3.6.3.14), at 185,907 to 187,307 GFF3687 GFF3688 - ATP synthase epsilon chain (EC 3.6.3.14), at 187,320 to 187,739 GFF3688 GFF3689 - FIG01198523: hypothetical protein, at 187,944 to 189,146 GFF3689 GFF3690 - ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose) / ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose), at 189,143 to 190,690 GFF3690 Position (kb) 187 188 189 190Strain fitness (log2 ratio) -2 -1 0 1at 187.305 kb on + strandat 187.659 kb on + strand, within GFF3688at 187.722 kb on + strandat 187.722 kb on + strandat 187.722 kb on + strandat 187.722 kb on + strandat 187.722 kb on + strandat 187.723 kb on - strandat 187.723 kb on - strandat 187.723 kb on - strandat 187.737 kb on + strandat 187.737 kb on + strandat 187.737 kb on + strandat 187.738 kb on - strandat 187.738 kb on - strandat 187.738 kb on - strandat 187.738 kb on - strandat 187.738 kb on - strandat 187.738 kb on - strandat 187.738 kb on - strandat 187.738 kb on - strandat 187.738 kb on - strandat 187.873 kb on + strandat 187.873 kb on + strandat 187.873 kb on + strandat 187.873 kb on + strandat 187.873 kb on + strandat 187.873 kb on + strandat 187.873 kb on + strandat 187.874 kb on - strandat 187.874 kb on - strandat 187.874 kb on - strandat 187.876 kb on + strandat 187.877 kb on - strandat 188.311 kb on + strand, within GFF3689at 188.312 kb on - strand, within GFF3689at 188.312 kb on - strand, within GFF3689at 188.312 kb on - strand, within GFF3689at 188.464 kb on - strand, within GFF3689at 188.464 kb on - strand, within GFF3689at 188.479 kb on - strand, within GFF3689at 188.479 kb on - strand, within GFF3689at 188.622 kb on + strand, within GFF3689at 188.622 kb on + strand, within GFF3689at 188.623 kb on - strand, within GFF3689at 189.012 kb on + strand, within GFF3689at 189.012 kb on + strand, within GFF3689at 189.012 kb on + strand, within GFF3689at 189.013 kb on - strand, within GFF3689at 189.013 kb on - strand, within GFF3689at 189.057 kb on + strandat 189.057 kb on + strandat 189.057 kb on + strandat 189.057 kb on + strandat 189.057 kb on + strandat 189.057 kb on + strandat 189.058 kb on - strandat 189.058 kb on - strandat 189.269 kb on + strandat 189.269 kb on + strandat 189.269 kb on + strandat 189.269 kb on + strandat 189.755 kb on + strand, within GFF3690at 189.755 kb on + strand, within GFF3690at 189.755 kb on + strand, within GFF3690at 189.755 kb on + strand, within GFF3690at 189.755 kb on + strand, within GFF3690at 189.755 kb on + strand, within GFF3690at 189.755 kb on + strand, within GFF3690at 189.756 kb on - strand, within GFF3690at 189.756 kb on - strand, within GFF3690at 189.756 kb on - strand, within GFF3690at 189.756 kb on - strand, within GFF3690at 189.756 kb on - strand, within GFF3690at 189.756 kb on - strand, within GFF3690

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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187,305 + +0.2
187,659 + GFF3688 0.81 +0.2
187,722 + -1.2
187,722 + +0.2
187,722 + -0.8
187,722 + +0.0
187,722 + -0.4
187,723 - -1.6
187,723 - -0.8
187,723 - +0.6
187,737 + -2.6
187,737 + +0.0
187,737 + -0.1
187,738 - -0.4
187,738 - -0.4
187,738 - -0.1
187,738 - -0.4
187,738 - -0.7
187,738 - -0.2
187,738 - +0.9
187,738 - +0.2
187,738 - +0.3
187,873 + +0.4
187,873 + +1.2
187,873 + +0.2
187,873 + +0.3
187,873 + -0.9
187,873 + +1.3
187,873 + +0.1
187,874 - +0.4
187,874 - +0.9
187,874 - +0.2
187,876 + +0.2
187,877 - +0.9
188,311 + GFF3689 0.31 -0.2
188,312 - GFF3689 0.31 +0.0
188,312 - GFF3689 0.31 +0.1
188,312 - GFF3689 0.31 +0.4
188,464 - GFF3689 0.43 -0.7
188,464 - GFF3689 0.43 -1.6
188,479 - GFF3689 0.44 +0.2
188,479 - GFF3689 0.44 -1.4
188,622 + GFF3689 0.56 +1.6
188,622 + GFF3689 0.56 +0.2
188,623 - GFF3689 0.56 +0.9
189,012 + GFF3689 0.89 -0.5
189,012 + GFF3689 0.89 +1.2
189,012 + GFF3689 0.89 +0.2
189,013 - GFF3689 0.89 +1.2
189,013 - GFF3689 0.89 +0.2
189,057 + +1.7
189,057 + -0.3
189,057 + -0.6
189,057 + -1.3
189,057 + +0.3
189,057 + -0.5
189,058 - -1.0
189,058 - +0.3
189,269 + -0.2
189,269 + +0.3
189,269 + -0.0
189,269 + +0.1
189,755 + GFF3690 0.40 +0.4
189,755 + GFF3690 0.40 -0.8
189,755 + GFF3690 0.40 -0.2
189,755 + GFF3690 0.40 -0.4
189,755 + GFF3690 0.40 -1.2
189,755 + GFF3690 0.40 -0.7
189,755 + GFF3690 0.40 +0.6
189,756 - GFF3690 0.40 -0.2
189,756 - GFF3690 0.40 -0.2
189,756 - GFF3690 0.40 -0.5
189,756 - GFF3690 0.40 +0.5
189,756 - GFF3690 0.40 -0.7
189,756 - GFF3690 0.40 +1.6

Or see this region's nucleotide sequence