Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3560 and GFF3561 are separated by 36 nucleotides GFF3561 and GFF3562 are separated by 96 nucleotides
GFF3560 - hypothetical protein, at 71,021 to 72,040
GFF3560
GFF3561 - Glycosyltransferases involved in cell wall biogenesis, at 72,077 to 73,105
GFF3561
GFF3562 - UDP-glucose 6-dehydrogenase (EC 1.1.1.22), at 73,202 to 74,542
GFF3562
Position (kb)
72
73
74 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 71.172 kb on + strand, within GFF3560 at 71.172 kb on + strand, within GFF3560 at 71.172 kb on + strand, within GFF3560 at 71.172 kb on + strand, within GFF3560 at 71.172 kb on + strand, within GFF3560 at 71.172 kb on + strand, within GFF3560 at 71.173 kb on - strand, within GFF3560 at 71.173 kb on - strand, within GFF3560 at 71.173 kb on - strand, within GFF3560 at 71.173 kb on - strand, within GFF3560 at 71.173 kb on - strand, within GFF3560 at 71.173 kb on - strand, within GFF3560 at 71.173 kb on - strand, within GFF3560 at 71.173 kb on - strand, within GFF3560 at 71.269 kb on - strand, within GFF3560 at 71.604 kb on + strand, within GFF3560 at 71.604 kb on + strand, within GFF3560 at 71.604 kb on + strand, within GFF3560 at 71.604 kb on + strand, within GFF3560 at 71.605 kb on - strand, within GFF3560 at 71.605 kb on - strand, within GFF3560 at 71.605 kb on - strand, within GFF3560 at 71.788 kb on - strand, within GFF3560 at 72.090 kb on + strand at 72.090 kb on + strand at 72.090 kb on + strand at 72.091 kb on - strand at 72.091 kb on - strand at 72.342 kb on + strand, within GFF3561 at 72.342 kb on + strand, within GFF3561 at 72.342 kb on + strand, within GFF3561 at 72.342 kb on + strand, within GFF3561 at 72.343 kb on - strand, within GFF3561 at 72.343 kb on - strand, within GFF3561 at 72.343 kb on - strand, within GFF3561 at 72.343 kb on - strand, within GFF3561 at 72.343 kb on - strand, within GFF3561 at 72.343 kb on - strand, within GFF3561 at 72.372 kb on + strand, within GFF3561 at 72.372 kb on + strand, within GFF3561 at 72.372 kb on + strand, within GFF3561 at 72.372 kb on + strand, within GFF3561 at 72.373 kb on - strand, within GFF3561 at 72.373 kb on - strand, within GFF3561 at 72.373 kb on - strand, within GFF3561 at 72.373 kb on - strand, within GFF3561 at 72.373 kb on - strand, within GFF3561 at 72.373 kb on - strand, within GFF3561 at 72.373 kb on - strand, within GFF3561 at 72.373 kb on - strand, within GFF3561 at 72.373 kb on - strand, within GFF3561 at 72.460 kb on - strand, within GFF3561 at 72.520 kb on - strand, within GFF3561 at 72.520 kb on - strand, within GFF3561 at 72.540 kb on + strand, within GFF3561 at 72.540 kb on + strand, within GFF3561 at 72.540 kb on + strand, within GFF3561 at 72.540 kb on + strand, within GFF3561 at 72.541 kb on - strand, within GFF3561 at 72.541 kb on - strand, within GFF3561 at 72.541 kb on - strand, within GFF3561 at 72.541 kb on - strand, within GFF3561 at 72.588 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.630 kb on + strand, within GFF3561 at 72.631 kb on - strand, within GFF3561 at 72.631 kb on - strand, within GFF3561 at 72.631 kb on - strand, within GFF3561 at 72.631 kb on - strand, within GFF3561 at 72.631 kb on - strand, within GFF3561 at 72.631 kb on - strand, within GFF3561 at 72.631 kb on - strand, within GFF3561 at 72.631 kb on - strand, within GFF3561 at 72.843 kb on + strand, within GFF3561 at 72.843 kb on + strand, within GFF3561 at 72.843 kb on + strand, within GFF3561 at 72.844 kb on - strand, within GFF3561 at 72.844 kb on - strand, within GFF3561 at 72.844 kb on - strand, within GFF3561 at 72.846 kb on + strand, within GFF3561 at 73.153 kb on + strand at 73.154 kb on - strand at 73.229 kb on + strand at 73.230 kb on - strand at 73.763 kb on + strand, within GFF3562 at 73.835 kb on + strand, within GFF3562 at 73.835 kb on + strand, within GFF3562 at 73.836 kb on - strand, within GFF3562 at 73.836 kb on - strand, within GFF3562 at 73.988 kb on + strand, within GFF3562
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 71,172 + GFF3560 0.15 +0.2 71,172 + GFF3560 0.15 -0.5 71,172 + GFF3560 0.15 +0.0 71,172 + GFF3560 0.15 +0.3 71,172 + GFF3560 0.15 +1.0 71,172 + GFF3560 0.15 +0.1 71,173 - GFF3560 0.15 -1.2 71,173 - GFF3560 0.15 -0.8 71,173 - GFF3560 0.15 -0.5 71,173 - GFF3560 0.15 -1.1 71,173 - GFF3560 0.15 -2.2 71,173 - GFF3560 0.15 -0.2 71,173 - GFF3560 0.15 -0.3 71,173 - GFF3560 0.15 -0.3 71,269 - GFF3560 0.24 -1.4 71,604 + GFF3560 0.57 -0.7 71,604 + GFF3560 0.57 -0.7 71,604 + GFF3560 0.57 +0.4 71,604 + GFF3560 0.57 -0.4 71,605 - GFF3560 0.57 -1.5 71,605 - GFF3560 0.57 +0.7 71,605 - GFF3560 0.57 -0.3 71,788 - GFF3560 0.75 +0.4 72,090 + -0.5 72,090 + +0.3 72,090 + -0.1 72,091 - +1.2 72,091 - -0.3 72,342 + GFF3561 0.26 -0.5 72,342 + GFF3561 0.26 -0.8 72,342 + GFF3561 0.26 -0.8 72,342 + GFF3561 0.26 -0.2 72,343 - GFF3561 0.26 +0.4 72,343 - GFF3561 0.26 +0.3 72,343 - GFF3561 0.26 -0.7 72,343 - GFF3561 0.26 -0.4 72,343 - GFF3561 0.26 -1.4 72,343 - GFF3561 0.26 +0.8 72,372 + GFF3561 0.29 -0.3 72,372 + GFF3561 0.29 -2.4 72,372 + GFF3561 0.29 -0.8 72,372 + GFF3561 0.29 -0.6 72,373 - GFF3561 0.29 +3.5 72,373 - GFF3561 0.29 -0.2 72,373 - GFF3561 0.29 +0.2 72,373 - GFF3561 0.29 -1.2 72,373 - GFF3561 0.29 -0.0 72,373 - GFF3561 0.29 +1.2 72,373 - GFF3561 0.29 -0.0 72,373 - GFF3561 0.29 +0.4 72,373 - GFF3561 0.29 +0.9 72,460 - GFF3561 0.37 -1.0 72,520 - GFF3561 0.43 -1.3 72,520 - GFF3561 0.43 -0.8 72,540 + GFF3561 0.45 -0.3 72,540 + GFF3561 0.45 -0.1 72,540 + GFF3561 0.45 -0.4 72,540 + GFF3561 0.45 -0.1 72,541 - GFF3561 0.45 -1.4 72,541 - GFF3561 0.45 +0.8 72,541 - GFF3561 0.45 -0.3 72,541 - GFF3561 0.45 +0.6 72,588 + GFF3561 0.50 -1.5 72,630 + GFF3561 0.54 +2.2 72,630 + GFF3561 0.54 +0.2 72,630 + GFF3561 0.54 -0.2 72,630 + GFF3561 0.54 -1.0 72,630 + GFF3561 0.54 -0.1 72,630 + GFF3561 0.54 +0.0 72,630 + GFF3561 0.54 +1.7 72,630 + GFF3561 0.54 +0.7 72,630 + GFF3561 0.54 -0.3 72,630 + GFF3561 0.54 -1.6 72,631 - GFF3561 0.54 -0.2 72,631 - GFF3561 0.54 -0.1 72,631 - GFF3561 0.54 +0.8 72,631 - GFF3561 0.54 -0.7 72,631 - GFF3561 0.54 -0.5 72,631 - GFF3561 0.54 +0.8 72,631 - GFF3561 0.54 -0.1 72,631 - GFF3561 0.54 -0.3 72,843 + GFF3561 0.74 -0.9 72,843 + GFF3561 0.74 +0.7 72,843 + GFF3561 0.74 +0.5 72,844 - GFF3561 0.75 +0.2 72,844 - GFF3561 0.75 -0.3 72,844 - GFF3561 0.75 +0.4 72,846 + GFF3561 0.75 -0.5 73,153 + +1.6 73,154 - +0.3 73,229 + -0.5 73,230 - +0.1 73,763 + GFF3562 0.42 +0.2 73,835 + GFF3562 0.47 -0.1 73,835 + GFF3562 0.47 -1.2 73,836 - GFF3562 0.47 -0.6 73,836 - GFF3562 0.47 -0.3 73,988 + GFF3562 0.59 +0.6
Or see this region's nucleotide sequence