Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3223 and GFF3224 overlap by 4 nucleotides GFF3224 and GFF3225 overlap by 4 nucleotides GFF3225 and GFF3226 are separated by 90 nucleotides GFF3226 and GFF3227 are separated by 17 nucleotides GFF3227 and GFF3228 are separated by 41 nucleotides
GFF3223 - no description, at 44,390 to 45,007
GFF3223
GFF3224 - no description, at 45,004 to 45,630
GFF3224
GFF3225 - hypothetical protein, at 45,627 to 47,885
GFF3225
GFF3226 - no description, at 47,976 to 48,326
GFF3226
GFF3227 - no description, at 48,344 to 48,694
GFF3227
GFF3228 - hypothetical protein, at 48,736 to 49,665
GFF3228
Position (kb) 45
46
47
48 Strain fitness (log2 ratio) -2
-1
0
1
2 at 45.566 kb on + strand, within GFF3224 at 45.566 kb on + strand, within GFF3224 at 45.567 kb on - strand, within GFF3224 at 45.928 kb on + strand, within GFF3225 at 46.133 kb on - strand, within GFF3225 at 46.133 kb on - strand, within GFF3225 at 47.024 kb on + strand, within GFF3225 at 47.120 kb on + strand, within GFF3225 at 47.120 kb on + strand, within GFF3225 at 47.120 kb on + strand, within GFF3225 at 47.134 kb on + strand, within GFF3225 at 47.135 kb on - strand, within GFF3225 at 47.135 kb on - strand, within GFF3225 at 47.369 kb on - strand, within GFF3225 at 47.635 kb on + strand, within GFF3225 at 47.636 kb on - strand, within GFF3225 at 47.636 kb on - strand, within GFF3225 at 47.665 kb on + strand at 47.710 kb on + strand at 47.710 kb on + strand at 47.710 kb on + strand at 47.826 kb on + strand at 47.826 kb on + strand at 47.891 kb on + strand at 47.892 kb on - strand at 48.192 kb on + strand, within GFF3226 at 48.192 kb on + strand, within GFF3226 at 48.193 kb on - strand, within GFF3226 at 48.381 kb on + strand, within GFF3227 at 48.381 kb on + strand, within GFF3227 at 48.387 kb on + strand, within GFF3227 at 48.388 kb on - strand, within GFF3227
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 45,566 + GFF3224 0.90 -0.1 45,566 + GFF3224 0.90 -0.4 45,567 - GFF3224 0.90 +0.1 45,928 + GFF3225 0.13 -2.4 46,133 - GFF3225 0.22 -1.2 46,133 - GFF3225 0.22 -1.8 47,024 + GFF3225 0.62 +0.8 47,120 + GFF3225 0.66 -0.8 47,120 + GFF3225 0.66 +1.5 47,120 + GFF3225 0.66 -0.1 47,134 + GFF3225 0.67 -0.7 47,135 - GFF3225 0.67 +0.1 47,135 - GFF3225 0.67 +0.0 47,369 - GFF3225 0.77 -0.1 47,635 + GFF3225 0.89 -0.4 47,636 - GFF3225 0.89 -1.2 47,636 - GFF3225 0.89 -1.7 47,665 + +0.6 47,710 + -1.3 47,710 + -1.0 47,710 + -0.4 47,826 + -0.1 47,826 + -1.5 47,891 + -1.0 47,892 - -1.4 48,192 + GFF3226 0.62 -0.3 48,192 + GFF3226 0.62 -1.3 48,193 - GFF3226 0.62 -0.1 48,381 + GFF3227 0.11 +0.6 48,381 + GFF3227 0.11 -1.0 48,387 + GFF3227 0.12 +0.4 48,388 - GFF3227 0.13 +2.2
Or see this region's nucleotide sequence