Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3120 and GFF3121 are separated by 258 nucleotides GFF3121 and GFF3122 are separated by 1 nucleotides GFF3122 and GFF3123 are separated by 39 nucleotides GFF3123 and GFF3124 are separated by 232 nucleotides GFF3124 and GFF3125 are separated by 4 nucleotides
GFF3120 - no description, at 548,398 to 549,240
GFF3120
GFF3121 - hypothetical protein, at 549,499 to 549,912
GFF3121
GFF3122 - ATP/GTP-binding protein, at 549,914 to 551,791
GFF3122
GFF3123 - no description, at 551,831 to 552,103
GFF3123
GFF3124 - no description, at 552,336 to 552,674
GFF3124
GFF3125 - no description, at 552,679 to 553,125
GFF3125
Position (kb) 549
550
551
552 Strain fitness (log2 ratio) -2
-1
0
1
2 at 548.915 kb on + strand, within GFF3120 at 548.916 kb on - strand, within GFF3120 at 549.042 kb on - strand, within GFF3120 at 549.042 kb on - strand, within GFF3120 at 549.078 kb on - strand, within GFF3120 at 549.083 kb on + strand, within GFF3120 at 549.135 kb on + strand, within GFF3120 at 549.135 kb on + strand, within GFF3120 at 549.136 kb on - strand, within GFF3120 at 549.140 kb on - strand, within GFF3120 at 549.140 kb on - strand, within GFF3120 at 549.140 kb on - strand, within GFF3120 at 549.140 kb on - strand, within GFF3120 at 549.140 kb on - strand, within GFF3120 at 549.140 kb on - strand, within GFF3120 at 549.164 kb on + strand at 549.164 kb on + strand at 549.165 kb on - strand at 549.165 kb on - strand at 549.165 kb on - strand at 549.165 kb on - strand at 549.193 kb on + strand at 549.194 kb on + strand at 549.195 kb on - strand at 549.195 kb on - strand at 549.195 kb on - strand at 549.195 kb on - strand at 549.195 kb on - strand at 549.195 kb on - strand at 549.198 kb on + strand at 549.198 kb on + strand at 549.198 kb on + strand at 549.198 kb on + strand at 549.198 kb on + strand at 549.198 kb on + strand at 549.198 kb on + strand at 549.198 kb on + strand at 549.199 kb on - strand at 549.199 kb on - strand at 549.199 kb on - strand at 549.199 kb on - strand at 549.258 kb on + strand at 549.320 kb on + strand at 549.320 kb on + strand at 549.321 kb on - strand at 549.321 kb on - strand at 549.321 kb on - strand at 549.321 kb on - strand at 549.382 kb on + strand at 549.383 kb on - strand at 549.438 kb on + strand at 549.438 kb on + strand at 549.439 kb on - strand at 549.439 kb on - strand at 549.444 kb on + strand at 549.444 kb on + strand at 549.444 kb on + strand at 549.444 kb on + strand at 549.444 kb on + strand at 549.445 kb on - strand at 549.445 kb on - strand at 549.445 kb on - strand at 549.540 kb on - strand at 549.623 kb on + strand, within GFF3121 at 549.623 kb on + strand, within GFF3121 at 549.624 kb on - strand, within GFF3121 at 549.758 kb on - strand, within GFF3121 at 549.855 kb on - strand, within GFF3121 at 549.923 kb on + strand at 549.995 kb on + strand at 549.995 kb on + strand at 550.061 kb on + strand at 550.062 kb on - strand at 550.062 kb on - strand at 550.062 kb on - strand at 550.062 kb on - strand at 550.074 kb on + strand at 550.074 kb on + strand at 550.209 kb on + strand, within GFF3122 at 550.210 kb on - strand, within GFF3122 at 550.237 kb on + strand, within GFF3122 at 550.237 kb on + strand, within GFF3122 at 550.238 kb on - strand, within GFF3122 at 550.238 kb on - strand, within GFF3122 at 550.262 kb on + strand, within GFF3122 at 550.262 kb on + strand, within GFF3122 at 550.263 kb on - strand, within GFF3122 at 550.263 kb on - strand, within GFF3122 at 550.263 kb on - strand, within GFF3122 at 550.263 kb on - strand, within GFF3122 at 550.302 kb on - strand, within GFF3122 at 550.317 kb on - strand, within GFF3122 at 550.317 kb on - strand, within GFF3122 at 550.317 kb on - strand, within GFF3122 at 550.371 kb on + strand, within GFF3122 at 550.371 kb on + strand, within GFF3122 at 550.372 kb on - strand, within GFF3122 at 550.385 kb on + strand, within GFF3122 at 550.385 kb on + strand, within GFF3122 at 550.393 kb on + strand, within GFF3122 at 550.398 kb on - strand, within GFF3122 at 550.398 kb on - strand, within GFF3122 at 550.431 kb on - strand, within GFF3122 at 550.439 kb on + strand, within GFF3122 at 550.440 kb on - strand, within GFF3122 at 550.440 kb on - strand, within GFF3122 at 550.563 kb on + strand, within GFF3122 at 550.796 kb on + strand, within GFF3122 at 550.796 kb on + strand, within GFF3122 at 550.797 kb on - strand, within GFF3122 at 550.797 kb on - strand, within GFF3122 at 550.869 kb on - strand, within GFF3122 at 551.177 kb on + strand, within GFF3122 at 551.177 kb on + strand, within GFF3122 at 551.177 kb on + strand, within GFF3122 at 551.178 kb on - strand, within GFF3122 at 551.505 kb on - strand, within GFF3122 at 551.505 kb on - strand, within GFF3122 at 551.673 kb on - strand at 551.708 kb on + strand at 551.708 kb on + strand at 551.714 kb on + strand at 551.714 kb on + strand at 551.741 kb on + strand at 551.741 kb on + strand at 551.742 kb on - strand at 551.790 kb on - strand at 552.088 kb on + strand at 552.127 kb on - strand at 552.161 kb on + strand at 552.253 kb on + strand at 552.253 kb on + strand at 552.253 kb on + strand at 552.358 kb on - strand at 552.699 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 548,915 + GFF3120 0.61 +0.1 548,916 - GFF3120 0.61 +0.4 549,042 - GFF3120 0.76 -1.4 549,042 - GFF3120 0.76 +0.2 549,078 - GFF3120 0.81 +0.3 549,083 + GFF3120 0.81 +0.1 549,135 + GFF3120 0.87 +0.6 549,135 + GFF3120 0.87 -1.3 549,136 - GFF3120 0.88 +0.1 549,140 - GFF3120 0.88 -0.7 549,140 - GFF3120 0.88 -1.0 549,140 - GFF3120 0.88 +0.5 549,140 - GFF3120 0.88 -0.0 549,140 - GFF3120 0.88 +1.9 549,140 - GFF3120 0.88 +1.0 549,164 + -0.5 549,164 + -0.5 549,165 - -1.4 549,165 - -1.6 549,165 - +0.0 549,165 - +0.2 549,193 + +0.1 549,194 + -1.0 549,195 - +1.1 549,195 - -0.8 549,195 - +1.4 549,195 - +0.1 549,195 - -0.9 549,195 - +1.4 549,198 + -1.9 549,198 + +0.9 549,198 + -0.8 549,198 + -0.2 549,198 + -0.5 549,198 + -0.3 549,198 + -0.0 549,198 + +0.2 549,199 - -0.0 549,199 - -1.8 549,199 - -0.2 549,199 - -1.0 549,258 + -0.4 549,320 + -0.7 549,320 + -0.6 549,321 - -0.6 549,321 - -0.8 549,321 - -0.5 549,321 - +1.3 549,382 + +0.4 549,383 - -0.6 549,438 + -0.4 549,438 + -0.6 549,439 - -1.3 549,439 - +0.6 549,444 + +0.7 549,444 + +0.2 549,444 + +0.8 549,444 + -0.1 549,444 + -0.3 549,445 - +0.2 549,445 - -0.0 549,445 - -0.8 549,540 - -0.7 549,623 + GFF3121 0.30 +0.1 549,623 + GFF3121 0.30 +0.3 549,624 - GFF3121 0.30 -0.3 549,758 - GFF3121 0.63 +0.6 549,855 - GFF3121 0.86 -1.1 549,923 + -1.0 549,995 + -0.5 549,995 + -0.4 550,061 + +1.2 550,062 - -1.0 550,062 - +0.2 550,062 - -0.3 550,062 - +0.2 550,074 + -0.8 550,074 + +0.3 550,209 + GFF3122 0.16 +0.7 550,210 - GFF3122 0.16 -1.3 550,237 + GFF3122 0.17 -1.6 550,237 + GFF3122 0.17 -0.8 550,238 - GFF3122 0.17 +1.1 550,238 - GFF3122 0.17 +0.4 550,262 + GFF3122 0.19 -0.1 550,262 + GFF3122 0.19 -0.6 550,263 - GFF3122 0.19 -0.9 550,263 - GFF3122 0.19 -1.2 550,263 - GFF3122 0.19 +0.3 550,263 - GFF3122 0.19 -0.5 550,302 - GFF3122 0.21 -0.7 550,317 - GFF3122 0.21 -0.6 550,317 - GFF3122 0.21 -0.6 550,317 - GFF3122 0.21 -0.9 550,371 + GFF3122 0.24 -1.0 550,371 + GFF3122 0.24 -0.5 550,372 - GFF3122 0.24 +0.6 550,385 + GFF3122 0.25 -0.9 550,385 + GFF3122 0.25 -1.1 550,393 + GFF3122 0.26 +0.1 550,398 - GFF3122 0.26 -0.3 550,398 - GFF3122 0.26 +0.6 550,431 - GFF3122 0.28 +0.6 550,439 + GFF3122 0.28 -1.2 550,440 - GFF3122 0.28 -0.0 550,440 - GFF3122 0.28 +0.4 550,563 + GFF3122 0.35 -1.6 550,796 + GFF3122 0.47 +0.3 550,796 + GFF3122 0.47 +0.3 550,797 - GFF3122 0.47 +0.2 550,797 - GFF3122 0.47 -0.9 550,869 - GFF3122 0.51 -0.5 551,177 + GFF3122 0.67 -1.8 551,177 + GFF3122 0.67 -0.6 551,177 + GFF3122 0.67 -1.1 551,178 - GFF3122 0.67 -0.7 551,505 - GFF3122 0.85 -0.4 551,505 - GFF3122 0.85 -0.4 551,673 - +0.1 551,708 + +0.9 551,708 + +0.2 551,714 + -1.2 551,714 + -0.1 551,741 + -0.9 551,741 + -0.5 551,742 - +0.1 551,790 - +0.1 552,088 + -0.8 552,127 - -0.7 552,161 + -0.6 552,253 + +0.1 552,253 + +0.2 552,253 + +1.9 552,358 - -0.1 552,699 - -0.7
Or see this region's nucleotide sequence