Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3067 and GFF3068 are separated by 28 nucleotides GFF3068 and GFF3069 are separated by 140 nucleotides
GFF3067 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 500,946 to 502,025
GFF3067
GFF3068 - FIG00538108: hypothetical protein, at 502,054 to 502,563
GFF3068
GFF3069 - Glutamine synthetase type I (EC 6.3.1.2), at 502,704 to 504,119
GFF3069
Position (kb) 502
503 Strain fitness (log2 ratio) -2
-1
0
1
2 at 501.078 kb on + strand, within GFF3067 at 501.089 kb on - strand, within GFF3067 at 501.214 kb on - strand, within GFF3067 at 501.508 kb on + strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.779 kb on - strand, within GFF3067 at 501.779 kb on - strand, within GFF3067 at 502.025 kb on + strand at 502.025 kb on + strand at 502.026 kb on - strand at 502.026 kb on - strand at 502.048 kb on + strand at 502.048 kb on + strand at 502.048 kb on + strand at 502.048 kb on + strand at 502.049 kb on - strand at 502.157 kb on + strand, within GFF3068 at 502.157 kb on + strand, within GFF3068 at 502.157 kb on + strand, within GFF3068 at 502.157 kb on + strand, within GFF3068 at 502.157 kb on + strand, within GFF3068 at 502.158 kb on - strand, within GFF3068 at 502.158 kb on - strand, within GFF3068 at 502.166 kb on + strand, within GFF3068 at 502.211 kb on + strand, within GFF3068 at 502.211 kb on + strand, within GFF3068 at 502.211 kb on + strand, within GFF3068 at 502.212 kb on - strand, within GFF3068 at 502.212 kb on - strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.470 kb on - strand, within GFF3068 at 502.470 kb on - strand, within GFF3068 at 502.472 kb on + strand, within GFF3068 at 502.568 kb on - strand at 502.568 kb on - strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 501,078 + GFF3067 0.12 +0.0 501,089 - GFF3067 0.13 -0.1 501,214 - GFF3067 0.25 +0.1 501,508 + GFF3067 0.52 -0.4 501,509 - GFF3067 0.52 +0.1 501,509 - GFF3067 0.52 -1.8 501,509 - GFF3067 0.52 -0.9 501,509 - GFF3067 0.52 +0.9 501,509 - GFF3067 0.52 +0.3 501,779 - GFF3067 0.77 +0.7 501,779 - GFF3067 0.77 +0.6 502,025 + -0.4 502,025 + -0.7 502,026 - -0.7 502,026 - -0.3 502,048 + -1.3 502,048 + +0.7 502,048 + -1.9 502,048 + +1.5 502,049 - +0.2 502,157 + GFF3068 0.20 -0.6 502,157 + GFF3068 0.20 +0.7 502,157 + GFF3068 0.20 -1.4 502,157 + GFF3068 0.20 -0.9 502,157 + GFF3068 0.20 -1.6 502,158 - GFF3068 0.20 -1.6 502,158 - GFF3068 0.20 -0.7 502,166 + GFF3068 0.22 -0.2 502,211 + GFF3068 0.31 -0.9 502,211 + GFF3068 0.31 -0.9 502,211 + GFF3068 0.31 -0.1 502,212 - GFF3068 0.31 -0.8 502,212 - GFF3068 0.31 +1.9 502,469 + GFF3068 0.81 -0.9 502,469 + GFF3068 0.81 -1.2 502,469 + GFF3068 0.81 -1.5 502,469 + GFF3068 0.81 -1.8 502,469 + GFF3068 0.81 -1.2 502,470 - GFF3068 0.82 -1.5 502,470 - GFF3068 0.82 -0.8 502,472 + GFF3068 0.82 -1.2 502,568 - -0.3 502,568 - -0.2 502,673 + -0.9 502,673 + -1.1 502,673 + -0.3 502,673 + +0.3 502,673 + +0.1 502,673 + -0.9 502,673 + +0.0 502,673 + +0.3 502,673 + -0.1 502,673 + +0.4 502,673 + +0.5 502,673 + +0.7 502,673 + -1.1 502,673 + +0.9 502,673 + +0.6 502,674 - -0.1 502,674 - -0.6 502,674 - +0.1 502,674 - +0.9 502,674 - -1.6 502,674 - -0.3 502,674 - -1.1
Or see this region's nucleotide sequence