Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2518 and GFF2519 overlap by 4 nucleotides GFF2519 and GFF2520 are separated by 13 nucleotides
GFF2518 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 578,809 to 580,626
GFF2518
GFF2519 - FIG016425: Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 580,623 to 581,291
GFF2519
GFF2520 - UPF0053 inner membrane protein YtfL, at 581,305 to 582,615
GFF2520
Position (kb)
580
581
582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 580.836 kb on + strand, within GFF2519 at 580.836 kb on + strand, within GFF2519 at 580.836 kb on + strand, within GFF2519 at 580.837 kb on - strand, within GFF2519 at 580.837 kb on - strand, within GFF2519 at 580.837 kb on - strand, within GFF2519 at 580.837 kb on - strand, within GFF2519 at 580.837 kb on - strand, within GFF2519 at 580.837 kb on - strand, within GFF2519 at 580.926 kb on + strand, within GFF2519 at 580.926 kb on + strand, within GFF2519 at 580.926 kb on + strand, within GFF2519 at 580.927 kb on - strand, within GFF2519 at 580.927 kb on - strand, within GFF2519 at 580.927 kb on - strand, within GFF2519 at 581.031 kb on + strand, within GFF2519 at 581.031 kb on + strand, within GFF2519 at 581.031 kb on + strand, within GFF2519 at 581.031 kb on + strand, within GFF2519 at 581.031 kb on + strand, within GFF2519 at 581.031 kb on + strand, within GFF2519 at 581.032 kb on - strand, within GFF2519 at 581.032 kb on - strand, within GFF2519 at 581.032 kb on - strand, within GFF2519 at 581.032 kb on - strand, within GFF2519 at 581.032 kb on - strand, within GFF2519 at 581.032 kb on - strand, within GFF2519 at 581.032 kb on - strand, within GFF2519 at 581.032 kb on - strand, within GFF2519 at 581.139 kb on + strand, within GFF2519 at 581.139 kb on + strand, within GFF2519 at 581.140 kb on - strand, within GFF2519 at 581.140 kb on - strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.166 kb on + strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.167 kb on - strand, within GFF2519 at 581.184 kb on + strand, within GFF2519 at 581.184 kb on + strand, within GFF2519 at 581.185 kb on - strand, within GFF2519 at 581.366 kb on + strand at 581.366 kb on + strand at 581.366 kb on + strand at 581.367 kb on - strand at 581.367 kb on - strand at 581.367 kb on - strand at 581.367 kb on - strand at 581.367 kb on - strand at 581.367 kb on - strand at 581.367 kb on - strand at 581.367 kb on - strand at 581.367 kb on - strand at 581.394 kb on + strand at 581.394 kb on + strand at 581.394 kb on + strand at 581.394 kb on + strand at 581.394 kb on + strand at 581.394 kb on + strand at 581.394 kb on + strand at 581.394 kb on + strand at 581.394 kb on + strand at 581.394 kb on + strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.395 kb on - strand at 581.396 kb on + strand at 581.396 kb on + strand at 581.396 kb on + strand at 581.397 kb on - strand at 581.474 kb on + strand, within GFF2520 at 581.474 kb on + strand, within GFF2520 at 581.474 kb on + strand, within GFF2520 at 581.475 kb on - strand, within GFF2520 at 581.475 kb on - strand, within GFF2520 at 581.475 kb on - strand, within GFF2520 at 581.475 kb on - strand, within GFF2520 at 581.475 kb on - strand, within GFF2520 at 581.663 kb on + strand, within GFF2520 at 581.663 kb on + strand, within GFF2520 at 581.663 kb on + strand, within GFF2520 at 581.663 kb on + strand, within GFF2520 at 581.663 kb on + strand, within GFF2520 at 581.664 kb on - strand, within GFF2520 at 581.664 kb on - strand, within GFF2520 at 581.664 kb on - strand, within GFF2520 at 581.664 kb on - strand, within GFF2520 at 581.664 kb on - strand, within GFF2520 at 581.664 kb on - strand, within GFF2520 at 581.721 kb on - strand, within GFF2520 at 581.834 kb on + strand, within GFF2520 at 581.834 kb on + strand, within GFF2520 at 581.835 kb on - strand, within GFF2520 at 581.835 kb on - strand, within GFF2520 at 581.873 kb on + strand, within GFF2520
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 580,836 + GFF2519 0.32 -1.1 580,836 + GFF2519 0.32 -0.3 580,836 + GFF2519 0.32 -1.5 580,837 - GFF2519 0.32 +0.2 580,837 - GFF2519 0.32 -0.4 580,837 - GFF2519 0.32 -2.0 580,837 - GFF2519 0.32 -1.2 580,837 - GFF2519 0.32 -1.0 580,837 - GFF2519 0.32 -1.9 580,926 + GFF2519 0.45 -2.5 580,926 + GFF2519 0.45 -0.5 580,926 + GFF2519 0.45 -1.0 580,927 - GFF2519 0.45 -0.8 580,927 - GFF2519 0.45 -1.9 580,927 - GFF2519 0.45 -1.0 581,031 + GFF2519 0.61 -1.7 581,031 + GFF2519 0.61 +0.2 581,031 + GFF2519 0.61 -1.1 581,031 + GFF2519 0.61 -0.5 581,031 + GFF2519 0.61 +0.5 581,031 + GFF2519 0.61 -1.5 581,032 - GFF2519 0.61 -2.4 581,032 - GFF2519 0.61 -2.2 581,032 - GFF2519 0.61 -1.8 581,032 - GFF2519 0.61 +1.0 581,032 - GFF2519 0.61 +0.5 581,032 - GFF2519 0.61 -0.3 581,032 - GFF2519 0.61 -0.7 581,032 - GFF2519 0.61 -2.2 581,139 + GFF2519 0.77 -1.4 581,139 + GFF2519 0.77 -1.4 581,140 - GFF2519 0.77 -1.3 581,140 - GFF2519 0.77 -0.5 581,166 + GFF2519 0.81 -1.2 581,166 + GFF2519 0.81 -1.9 581,166 + GFF2519 0.81 +0.7 581,166 + GFF2519 0.81 -1.5 581,166 + GFF2519 0.81 -0.7 581,166 + GFF2519 0.81 +0.5 581,166 + GFF2519 0.81 -0.4 581,166 + GFF2519 0.81 -1.1 581,166 + GFF2519 0.81 +0.6 581,166 + GFF2519 0.81 -1.5 581,166 + GFF2519 0.81 -1.3 581,166 + GFF2519 0.81 -1.4 581,167 - GFF2519 0.81 -0.7 581,167 - GFF2519 0.81 -2.3 581,167 - GFF2519 0.81 +0.1 581,167 - GFF2519 0.81 -1.0 581,167 - GFF2519 0.81 -1.2 581,167 - GFF2519 0.81 -1.3 581,167 - GFF2519 0.81 +1.0 581,167 - GFF2519 0.81 -1.6 581,167 - GFF2519 0.81 -0.8 581,167 - GFF2519 0.81 -0.9 581,167 - GFF2519 0.81 -0.3 581,167 - GFF2519 0.81 -0.9 581,167 - GFF2519 0.81 -1.7 581,167 - GFF2519 0.81 -1.0 581,167 - GFF2519 0.81 -0.6 581,167 - GFF2519 0.81 -0.4 581,167 - GFF2519 0.81 -0.9 581,167 - GFF2519 0.81 -2.5 581,167 - GFF2519 0.81 -1.3 581,167 - GFF2519 0.81 +0.0 581,167 - GFF2519 0.81 -1.2 581,184 + GFF2519 0.84 -0.9 581,184 + GFF2519 0.84 -2.0 581,185 - GFF2519 0.84 -2.6 581,366 + -0.7 581,366 + +0.0 581,366 + +0.7 581,367 - +0.5 581,367 - -1.2 581,367 - -1.2 581,367 - +0.4 581,367 - +1.8 581,367 - -0.9 581,367 - -0.8 581,367 - -3.2 581,367 - +1.3 581,394 + -0.5 581,394 + -0.2 581,394 + -2.7 581,394 + +0.0 581,394 + -0.8 581,394 + -0.2 581,394 + -0.5 581,394 + -0.4 581,394 + +0.4 581,394 + -0.9 581,395 - -0.3 581,395 - -0.5 581,395 - -0.3 581,395 - -0.9 581,395 - +0.0 581,395 - +0.0 581,395 - -1.5 581,395 - -1.1 581,395 - -0.7 581,395 - -0.1 581,395 - +0.0 581,396 + -1.1 581,396 + -1.9 581,396 + +0.0 581,397 - -2.3 581,474 + GFF2520 0.13 +1.3 581,474 + GFF2520 0.13 -1.9 581,474 + GFF2520 0.13 -0.3 581,475 - GFF2520 0.13 -0.6 581,475 - GFF2520 0.13 -1.3 581,475 - GFF2520 0.13 -1.1 581,475 - GFF2520 0.13 -1.9 581,475 - GFF2520 0.13 +0.6 581,663 + GFF2520 0.27 +1.0 581,663 + GFF2520 0.27 -0.5 581,663 + GFF2520 0.27 -1.4 581,663 + GFF2520 0.27 -0.6 581,663 + GFF2520 0.27 +0.8 581,664 - GFF2520 0.27 -0.5 581,664 - GFF2520 0.27 +0.1 581,664 - GFF2520 0.27 -1.5 581,664 - GFF2520 0.27 -1.1 581,664 - GFF2520 0.27 -0.6 581,664 - GFF2520 0.27 +0.5 581,721 - GFF2520 0.32 +0.8 581,834 + GFF2520 0.40 -2.4 581,834 + GFF2520 0.40 +1.0 581,835 - GFF2520 0.40 -0.2 581,835 - GFF2520 0.40 +0.0 581,873 + GFF2520 0.43 +1.0
Or see this region's nucleotide sequence