Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2487 and GFF2488 overlap by 4 nucleotides GFF2488 and GFF2489 are separated by 127 nucleotides GFF2489 and GFF2490 are separated by 7 nucleotides
GFF2487 - Two-component system sensor histidine kinase/response regulator hybrid, at 543,418 to 547,056
GFF2487
GFF2488 - Two-component transcriptional response regulator, LuxR family, at 547,053 to 548,093
GFF2488
GFF2489 - hypothetical protein, at 548,221 to 548,880
GFF2489
GFF2490 - Transcriptional regulator, LysR family, at 548,888 to 549,808
GFF2490
Position (kb)
547
548
549 Strain fitness (log2 ratio)
-1
0
1 at 546.067 kb on + strand, within GFF2487 at 546.107 kb on - strand, within GFF2487 at 546.904 kb on + strand at 546.904 kb on + strand at 546.905 kb on - strand at 546.905 kb on - strand at 547.109 kb on + strand at 547.212 kb on + strand, within GFF2488 at 547.212 kb on + strand, within GFF2488 at 547.377 kb on - strand, within GFF2488 at 547.437 kb on + strand, within GFF2488 at 547.437 kb on + strand, within GFF2488 at 547.437 kb on + strand, within GFF2488 at 547.438 kb on - strand, within GFF2488 at 547.645 kb on - strand, within GFF2488 at 547.645 kb on - strand, within GFF2488 at 547.914 kb on + strand, within GFF2488 at 547.914 kb on + strand, within GFF2488 at 547.915 kb on - strand, within GFF2488 at 548.004 kb on + strand at 548.004 kb on + strand at 548.004 kb on + strand at 548.004 kb on + strand at 548.004 kb on + strand at 548.146 kb on + strand at 548.146 kb on + strand at 548.146 kb on + strand at 548.146 kb on + strand at 548.146 kb on + strand at 548.147 kb on - strand at 548.147 kb on - strand at 548.147 kb on - strand at 548.147 kb on - strand at 548.147 kb on - strand at 548.147 kb on - strand at 548.323 kb on + strand, within GFF2489 at 548.323 kb on + strand, within GFF2489 at 548.323 kb on + strand, within GFF2489 at 548.323 kb on + strand, within GFF2489 at 548.323 kb on + strand, within GFF2489 at 548.324 kb on - strand, within GFF2489 at 548.324 kb on - strand, within GFF2489 at 548.899 kb on - strand at 549.012 kb on + strand, within GFF2490 at 549.012 kb on + strand, within GFF2490 at 549.012 kb on + strand, within GFF2490 at 549.012 kb on + strand, within GFF2490 at 549.012 kb on + strand, within GFF2490 at 549.013 kb on - strand, within GFF2490 at 549.013 kb on - strand, within GFF2490 at 549.013 kb on - strand, within GFF2490 at 549.013 kb on - strand, within GFF2490 at 549.057 kb on + strand, within GFF2490 at 549.058 kb on - strand, within GFF2490
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 546,067 + GFF2487 0.73 +0.2 546,107 - GFF2487 0.74 +0.0 546,904 + -0.8 546,904 + -0.7 546,905 - -0.2 546,905 - -1.7 547,109 + +0.3 547,212 + GFF2488 0.15 +0.5 547,212 + GFF2488 0.15 +0.3 547,377 - GFF2488 0.31 -0.3 547,437 + GFF2488 0.37 -0.0 547,437 + GFF2488 0.37 -0.0 547,437 + GFF2488 0.37 -0.2 547,438 - GFF2488 0.37 -0.0 547,645 - GFF2488 0.57 +0.5 547,645 - GFF2488 0.57 -0.0 547,914 + GFF2488 0.83 +0.9 547,914 + GFF2488 0.83 +0.2 547,915 - GFF2488 0.83 -0.5 548,004 + +0.2 548,004 + +0.0 548,004 + +0.0 548,004 + -0.7 548,004 + -0.1 548,146 + +0.3 548,146 + +0.7 548,146 + -0.5 548,146 + +0.1 548,146 + -1.1 548,147 - +0.2 548,147 - -0.6 548,147 - +0.3 548,147 - -1.5 548,147 - -0.3 548,147 - -1.1 548,323 + GFF2489 0.15 -0.4 548,323 + GFF2489 0.15 +0.4 548,323 + GFF2489 0.15 -0.6 548,323 + GFF2489 0.15 -1.7 548,323 + GFF2489 0.15 -0.7 548,324 - GFF2489 0.16 +0.1 548,324 - GFF2489 0.16 +0.2 548,899 - -1.4 549,012 + GFF2490 0.13 -0.3 549,012 + GFF2490 0.13 -0.4 549,012 + GFF2490 0.13 +0.5 549,012 + GFF2490 0.13 +1.0 549,012 + GFF2490 0.13 +0.6 549,013 - GFF2490 0.14 -0.9 549,013 - GFF2490 0.14 -0.2 549,013 - GFF2490 0.14 +1.6 549,013 - GFF2490 0.14 -1.3 549,057 + GFF2490 0.18 +0.5 549,058 - GFF2490 0.18 +0.5
Or see this region's nucleotide sequence