Strain Fitness in Variovorax sp. SCN45 around GFF2487

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2485 and GFF2486 are separated by 25 nucleotidesGFF2486 and GFF2487 are separated by 104 nucleotidesGFF2487 and GFF2488 overlap by 4 nucleotides GFF2485 - Nickel-binding accessory protein UreJ-HupE, at 542,386 to 542,985 GFF2485 GFF2486 - Urease gamma subunit (EC 3.5.1.5), at 543,011 to 543,313 GFF2486 GFF2487 - Two-component system sensor histidine kinase/response regulator hybrid, at 543,418 to 547,056 GFF2487 GFF2488 - Two-component transcriptional response regulator, LuxR family, at 547,053 to 548,093 GFF2488 Position (kb) 543 544 545 546 547 548Strain fitness (log2 ratio) -2 -1 0 1 2at 543.219 kb on + strand, within GFF2486at 543.401 kb on + strandat 543.502 kb on + strandat 543.503 kb on - strandat 543.538 kb on + strandat 543.538 kb on + strandat 543.539 kb on - strandat 543.539 kb on - strandat 543.539 kb on - strandat 543.539 kb on - strandat 543.550 kb on + strandat 543.661 kb on + strandat 543.661 kb on + strandat 543.662 kb on - strandat 543.662 kb on - strandat 543.662 kb on - strandat 543.740 kb on - strandat 543.740 kb on - strandat 543.827 kb on - strand, within GFF2487at 543.827 kb on - strand, within GFF2487at 543.827 kb on - strand, within GFF2487at 543.827 kb on - strand, within GFF2487at 543.878 kb on - strand, within GFF2487at 543.919 kb on + strand, within GFF2487at 543.920 kb on - strand, within GFF2487at 543.920 kb on - strand, within GFF2487at 544.036 kb on + strand, within GFF2487at 544.037 kb on - strand, within GFF2487at 544.037 kb on - strand, within GFF2487at 544.082 kb on - strand, within GFF2487at 544.082 kb on - strand, within GFF2487at 544.186 kb on + strand, within GFF2487at 544.186 kb on + strand, within GFF2487at 544.313 kb on - strand, within GFF2487at 544.369 kb on + strand, within GFF2487at 544.369 kb on + strand, within GFF2487at 544.370 kb on - strand, within GFF2487at 544.370 kb on - strand, within GFF2487at 544.370 kb on - strand, within GFF2487at 544.370 kb on - strand, within GFF2487at 544.387 kb on + strand, within GFF2487at 544.387 kb on + strand, within GFF2487at 544.387 kb on + strand, within GFF2487at 544.388 kb on - strand, within GFF2487at 544.402 kb on + strand, within GFF2487at 544.402 kb on + strand, within GFF2487at 544.402 kb on + strand, within GFF2487at 544.403 kb on - strand, within GFF2487at 544.403 kb on - strand, within GFF2487at 544.403 kb on - strand, within GFF2487at 544.474 kb on + strand, within GFF2487at 544.474 kb on + strand, within GFF2487at 544.475 kb on - strand, within GFF2487at 544.900 kb on + strand, within GFF2487at 544.901 kb on - strand, within GFF2487at 544.901 kb on - strand, within GFF2487at 544.901 kb on - strand, within GFF2487at 544.901 kb on - strand, within GFF2487at 544.901 kb on - strand, within GFF2487at 544.903 kb on + strand, within GFF2487at 544.903 kb on + strand, within GFF2487at 544.903 kb on + strand, within GFF2487at 545.015 kb on - strand, within GFF2487at 545.015 kb on - strand, within GFF2487at 545.164 kb on - strand, within GFF2487at 545.164 kb on - strand, within GFF2487at 545.164 kb on - strand, within GFF2487at 545.425 kb on + strand, within GFF2487at 545.425 kb on + strand, within GFF2487at 545.425 kb on + strand, within GFF2487at 545.425 kb on + strand, within GFF2487at 545.425 kb on + strand, within GFF2487at 545.426 kb on - strand, within GFF2487at 545.686 kb on + strand, within GFF2487at 545.687 kb on - strand, within GFF2487at 545.687 kb on - strand, within GFF2487at 546.067 kb on + strand, within GFF2487at 546.107 kb on - strand, within GFF2487at 546.904 kb on + strandat 546.904 kb on + strandat 546.905 kb on - strandat 546.905 kb on - strandat 547.109 kb on + strandat 547.212 kb on + strand, within GFF2488at 547.212 kb on + strand, within GFF2488at 547.377 kb on - strand, within GFF2488at 547.437 kb on + strand, within GFF2488at 547.437 kb on + strand, within GFF2488at 547.437 kb on + strand, within GFF2488at 547.438 kb on - strand, within GFF2488at 547.645 kb on - strand, within GFF2488at 547.645 kb on - strand, within GFF2488at 547.914 kb on + strand, within GFF2488at 547.914 kb on + strand, within GFF2488at 547.915 kb on - strand, within GFF2488at 548.004 kb on + strandat 548.004 kb on + strandat 548.004 kb on + strandat 548.004 kb on + strandat 548.004 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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543,219 + GFF2486 0.69 -0.4
543,401 + +0.3
543,502 + +0.3
543,503 - -1.4
543,538 + -0.7
543,538 + -0.8
543,539 - +0.8
543,539 - -2.0
543,539 - +2.0
543,539 - +1.1
543,550 + -0.1
543,661 + -0.3
543,661 + -0.2
543,662 - +1.2
543,662 - +0.0
543,662 - +0.2
543,740 - +0.7
543,740 - +0.3
543,827 - GFF2487 0.11 +0.8
543,827 - GFF2487 0.11 +0.5
543,827 - GFF2487 0.11 -0.1
543,827 - GFF2487 0.11 +0.2
543,878 - GFF2487 0.13 +0.3
543,919 + GFF2487 0.14 -0.1
543,920 - GFF2487 0.14 -0.1
543,920 - GFF2487 0.14 -0.3
544,036 + GFF2487 0.17 +2.0
544,037 - GFF2487 0.17 -0.5
544,037 - GFF2487 0.17 -0.3
544,082 - GFF2487 0.18 -0.8
544,082 - GFF2487 0.18 +0.0
544,186 + GFF2487 0.21 -0.3
544,186 + GFF2487 0.21 +0.8
544,313 - GFF2487 0.25 +0.4
544,369 + GFF2487 0.26 -0.1
544,369 + GFF2487 0.26 +0.4
544,370 - GFF2487 0.26 -0.6
544,370 - GFF2487 0.26 -0.9
544,370 - GFF2487 0.26 +0.3
544,370 - GFF2487 0.26 -1.5
544,387 + GFF2487 0.27 +0.4
544,387 + GFF2487 0.27 -0.8
544,387 + GFF2487 0.27 +0.5
544,388 - GFF2487 0.27 -0.6
544,402 + GFF2487 0.27 -0.2
544,402 + GFF2487 0.27 -0.2
544,402 + GFF2487 0.27 -0.7
544,403 - GFF2487 0.27 +0.2
544,403 - GFF2487 0.27 -0.6
544,403 - GFF2487 0.27 -1.5
544,474 + GFF2487 0.29 -0.1
544,474 + GFF2487 0.29 -0.5
544,475 - GFF2487 0.29 +2.0
544,900 + GFF2487 0.41 +1.7
544,901 - GFF2487 0.41 -1.1
544,901 - GFF2487 0.41 -1.5
544,901 - GFF2487 0.41 -0.5
544,901 - GFF2487 0.41 +0.5
544,901 - GFF2487 0.41 +1.6
544,903 + GFF2487 0.41 +0.7
544,903 + GFF2487 0.41 +0.6
544,903 + GFF2487 0.41 +0.9
545,015 - GFF2487 0.44 +0.1
545,015 - GFF2487 0.44 +0.5
545,164 - GFF2487 0.48 +0.0
545,164 - GFF2487 0.48 -1.0
545,164 - GFF2487 0.48 -0.3
545,425 + GFF2487 0.55 +0.7
545,425 + GFF2487 0.55 -0.6
545,425 + GFF2487 0.55 +0.5
545,425 + GFF2487 0.55 +0.6
545,425 + GFF2487 0.55 -2.0
545,426 - GFF2487 0.55 -0.1
545,686 + GFF2487 0.62 -0.7
545,687 - GFF2487 0.62 -1.0
545,687 - GFF2487 0.62 +0.7
546,067 + GFF2487 0.73 +0.2
546,107 - GFF2487 0.74 +0.0
546,904 + -0.8
546,904 + -0.7
546,905 - -0.2
546,905 - -1.7
547,109 + +0.3
547,212 + GFF2488 0.15 +0.5
547,212 + GFF2488 0.15 +0.3
547,377 - GFF2488 0.31 -0.3
547,437 + GFF2488 0.37 -0.0
547,437 + GFF2488 0.37 -0.0
547,437 + GFF2488 0.37 -0.2
547,438 - GFF2488 0.37 -0.0
547,645 - GFF2488 0.57 +0.5
547,645 - GFF2488 0.57 -0.0
547,914 + GFF2488 0.83 +0.9
547,914 + GFF2488 0.83 +0.2
547,915 - GFF2488 0.83 -0.5
548,004 + +0.2
548,004 + +0.0
548,004 + +0.0
548,004 + -0.7
548,004 + -0.1

Or see this region's nucleotide sequence