Strain Fitness in Variovorax sp. SCN45 around GFF233

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF232 and GFF233 are separated by 206 nucleotidesGFF233 and GFF234 are separated by 37 nucleotides GFF232 - Arginine decarboxylase (EC 4.1.1.19); Ornithine decarboxylase (EC 4.1.1.17); Lysine decarboxylase (EC 4.1.1.18), at 260,333 to 262,639 GFF232 GFF233 - Putative activity regulator of membrane protease YbbK, at 262,846 to 263,271 GFF233 GFF234 - Protein QmcA (possibly involved in integral membrane quality control), at 263,309 to 264,238 GFF234 Position (kb) 262 263 264Strain fitness (log2 ratio) -2 -1 0 1 2at 262.150 kb on - strand, within GFF232at 262.150 kb on - strand, within GFF232at 262.167 kb on + strand, within GFF232at 262.212 kb on + strand, within GFF232at 262.212 kb on + strand, within GFF232at 262.212 kb on + strand, within GFF232at 262.213 kb on - strand, within GFF232at 262.213 kb on - strand, within GFF232at 262.338 kb on + strand, within GFF232at 262.338 kb on + strand, within GFF232at 262.339 kb on - strand, within GFF232at 262.339 kb on - strand, within GFF232at 262.344 kb on + strand, within GFF232at 262.344 kb on + strand, within GFF232at 262.344 kb on + strand, within GFF232at 262.344 kb on + strand, within GFF232at 262.344 kb on + strand, within GFF232at 262.345 kb on - strand, within GFF232at 262.345 kb on - strand, within GFF232at 262.345 kb on - strand, within GFF232at 262.345 kb on - strand, within GFF232at 262.345 kb on - strand, within GFF232at 262.345 kb on - strand, within GFF232at 262.345 kb on - strand, within GFF232at 262.345 kb on - strand, within GFF232at 262.509 kb on + strandat 262.510 kb on - strandat 262.510 kb on - strandat 262.683 kb on - strandat 262.683 kb on - strandat 262.683 kb on - strandat 262.683 kb on - strandat 262.697 kb on + strandat 262.766 kb on + strandat 262.766 kb on + strandat 262.767 kb on - strandat 262.767 kb on - strandat 262.772 kb on + strandat 262.918 kb on + strand, within GFF233at 262.918 kb on + strand, within GFF233at 262.918 kb on + strand, within GFF233at 263.038 kb on + strand, within GFF233at 263.038 kb on + strand, within GFF233at 263.039 kb on - strand, within GFF233at 263.269 kb on + strandat 263.269 kb on + strandat 263.270 kb on - strandat 263.270 kb on - strandat 263.284 kb on + strandat 263.284 kb on + strandat 263.285 kb on - strandat 263.420 kb on + strand, within GFF234at 263.420 kb on + strand, within GFF234at 263.420 kb on + strand, within GFF234at 263.420 kb on + strand, within GFF234at 263.420 kb on + strand, within GFF234at 263.421 kb on - strand, within GFF234at 263.480 kb on + strand, within GFF234at 263.480 kb on + strand, within GFF234at 263.481 kb on - strand, within GFF234at 263.481 kb on - strand, within GFF234at 263.573 kb on + strand, within GFF234at 263.573 kb on + strand, within GFF234at 263.573 kb on + strand, within GFF234at 263.573 kb on + strand, within GFF234at 263.573 kb on + strand, within GFF234at 263.573 kb on + strand, within GFF234at 263.574 kb on - strand, within GFF234at 263.574 kb on - strand, within GFF234at 263.607 kb on - strand, within GFF234at 263.607 kb on - strand, within GFF234at 263.607 kb on - strand, within GFF234at 263.607 kb on - strand, within GFF234at 263.621 kb on + strand, within GFF234at 263.621 kb on + strand, within GFF234at 263.621 kb on + strand, within GFF234at 263.621 kb on + strand, within GFF234at 263.621 kb on + strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.622 kb on - strand, within GFF234at 263.781 kb on - strand, within GFF234at 264.113 kb on + strand, within GFF234at 264.114 kb on - strand, within GFF234at 264.114 kb on - strand, within GFF234at 264.248 kb on + strandat 264.248 kb on + strandat 264.248 kb on + strandat 264.248 kb on + strandat 264.248 kb on + strandat 264.248 kb on + strandat 264.249 kb on - strandat 264.249 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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262,150 - GFF232 0.79 +1.0
262,150 - GFF232 0.79 -0.1
262,167 + GFF232 0.79 +0.4
262,212 + GFF232 0.81 -0.2
262,212 + GFF232 0.81 -0.3
262,212 + GFF232 0.81 +0.8
262,213 - GFF232 0.81 -0.4
262,213 - GFF232 0.81 +0.6
262,338 + GFF232 0.87 -1.0
262,338 + GFF232 0.87 -1.2
262,339 - GFF232 0.87 +0.5
262,339 - GFF232 0.87 -0.2
262,344 + GFF232 0.87 +1.9
262,344 + GFF232 0.87 +0.9
262,344 + GFF232 0.87 +0.4
262,344 + GFF232 0.87 +0.4
262,344 + GFF232 0.87 +0.2
262,345 - GFF232 0.87 +1.0
262,345 - GFF232 0.87 -0.9
262,345 - GFF232 0.87 -1.7
262,345 - GFF232 0.87 +1.1
262,345 - GFF232 0.87 +1.0
262,345 - GFF232 0.87 +1.0
262,345 - GFF232 0.87 +0.4
262,345 - GFF232 0.87 -0.6
262,509 + +1.3
262,510 - +0.4
262,510 - +1.6
262,683 - +0.0
262,683 - +0.1
262,683 - +0.1
262,683 - +1.4
262,697 + -0.8
262,766 + -1.6
262,766 + -0.8
262,767 - +0.0
262,767 - +0.9
262,772 + -0.6
262,918 + GFF233 0.17 -0.3
262,918 + GFF233 0.17 -0.3
262,918 + GFF233 0.17 +0.1
263,038 + GFF233 0.45 +1.5
263,038 + GFF233 0.45 -0.5
263,039 - GFF233 0.45 +0.6
263,269 + -0.4
263,269 + -0.2
263,270 - +0.4
263,270 - +0.6
263,284 + -1.1
263,284 + +0.5
263,285 - +0.6
263,420 + GFF234 0.12 -1.7
263,420 + GFF234 0.12 -0.6
263,420 + GFF234 0.12 +1.0
263,420 + GFF234 0.12 +0.8
263,420 + GFF234 0.12 -1.3
263,421 - GFF234 0.12 +0.8
263,480 + GFF234 0.18 -0.7
263,480 + GFF234 0.18 -1.5
263,481 - GFF234 0.18 -1.2
263,481 - GFF234 0.18 -0.3
263,573 + GFF234 0.28 -0.9
263,573 + GFF234 0.28 -2.2
263,573 + GFF234 0.28 -0.1
263,573 + GFF234 0.28 -0.6
263,573 + GFF234 0.28 +0.1
263,573 + GFF234 0.28 -0.5
263,574 - GFF234 0.28 -0.1
263,574 - GFF234 0.28 +0.6
263,607 - GFF234 0.32 +0.3
263,607 - GFF234 0.32 -0.3
263,607 - GFF234 0.32 +0.1
263,607 - GFF234 0.32 -0.9
263,621 + GFF234 0.34 +0.7
263,621 + GFF234 0.34 -1.0
263,621 + GFF234 0.34 +0.4
263,621 + GFF234 0.34 -0.4
263,621 + GFF234 0.34 -1.0
263,622 - GFF234 0.34 -1.0
263,622 - GFF234 0.34 -0.1
263,622 - GFF234 0.34 -1.9
263,622 - GFF234 0.34 -0.3
263,622 - GFF234 0.34 -0.5
263,622 - GFF234 0.34 -0.5
263,622 - GFF234 0.34 -1.5
263,622 - GFF234 0.34 +0.1
263,622 - GFF234 0.34 -1.3
263,622 - GFF234 0.34 +0.3
263,781 - GFF234 0.51 -0.6
264,113 + GFF234 0.86 +0.0
264,114 - GFF234 0.87 +0.8
264,114 - GFF234 0.87 -1.9
264,248 + -0.4
264,248 + -0.4
264,248 + -0.1
264,248 + +0.4
264,248 + +0.6
264,248 + -1.4
264,249 - -1.3
264,249 - -0.5

Or see this region's nucleotide sequence