Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2137 and GFF2138 are separated by 36 nucleotides GFF2138 and GFF2139 overlap by 4 nucleotides
GFF2137 - Ribose operon repressor, at 185,586 to 186,683
GFF2137
GFF2138 - Ribose ABC transporter, permease protein RbsC (TC 3.A.1.2.1), at 186,720 to 187,709
GFF2138
GFF2139 - Ribose ABC transporter, ATP-binding protein RbsA (TC 3.A.1.2.1), at 187,706 to 189,334
GFF2139
Position (kb)
186
187
188 Strain fitness (log2 ratio)
-1
0
1 at 185.828 kb on - strand, within GFF2137 at 186.365 kb on - strand, within GFF2137 at 186.415 kb on + strand, within GFF2137 at 186.466 kb on + strand, within GFF2137 at 186.467 kb on - strand, within GFF2137 at 186.467 kb on - strand, within GFF2137 at 186.467 kb on - strand, within GFF2137 at 186.550 kb on + strand, within GFF2137 at 186.550 kb on + strand, within GFF2137 at 186.550 kb on + strand, within GFF2137 at 186.551 kb on - strand, within GFF2137 at 186.551 kb on - strand, within GFF2137 at 186.701 kb on - strand at 187.270 kb on + strand, within GFF2138 at 187.270 kb on + strand, within GFF2138 at 187.271 kb on - strand, within GFF2138 at 187.582 kb on + strand, within GFF2138 at 187.583 kb on - strand, within GFF2138 at 187.583 kb on - strand, within GFF2138 at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.694 kb on + strand at 187.998 kb on + strand, within GFF2139 at 188.090 kb on + strand, within GFF2139
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 185,828 - GFF2137 0.22 -0.1 186,365 - GFF2137 0.71 +0.1 186,415 + GFF2137 0.76 -0.4 186,466 + GFF2137 0.80 +0.4 186,467 - GFF2137 0.80 -0.5 186,467 - GFF2137 0.80 +0.5 186,467 - GFF2137 0.80 +1.6 186,550 + GFF2137 0.88 -0.2 186,550 + GFF2137 0.88 -0.6 186,550 + GFF2137 0.88 -0.1 186,551 - GFF2137 0.88 -1.2 186,551 - GFF2137 0.88 -0.7 186,701 - +1.1 187,270 + GFF2138 0.56 +0.8 187,270 + GFF2138 0.56 +1.1 187,271 - GFF2138 0.56 -0.4 187,582 + GFF2138 0.87 -0.7 187,583 - GFF2138 0.87 +1.5 187,583 - GFF2138 0.87 -1.0 187,642 + -1.0 187,642 + -1.3 187,642 + -0.7 187,642 + +0.7 187,642 + +0.6 187,642 + -0.2 187,643 - -1.1 187,643 - +1.1 187,643 - +0.8 187,643 - +1.3 187,643 - +0.1 187,643 - -1.6 187,694 + -0.8 187,998 + GFF2139 0.18 -1.1 188,090 + GFF2139 0.24 -0.1
Or see this region's nucleotide sequence