Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1876 and GFF1877 are separated by 9 nucleotides GFF1877 and GFF1878 are separated by 33 nucleotides GFF1878 and GFF1879 are separated by 31 nucleotides
GFF1876 - Dihydrodipicolinate synthase family, at 549,594 to 550,487
GFF1876
GFF1877 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 550,497 to 551,279
GFF1877
GFF1878 - Bll6165 protein, at 551,313 to 551,723
GFF1878
GFF1879 - dicarboxylic acid transporter PcaT, at 551,755 to 553,101
GFF1879
Position (kb)
551
552 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 550.335 kb on + strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.490 kb on + strand at 550.490 kb on + strand at 550.490 kb on + strand at 550.491 kb on - strand at 550.951 kb on - strand, within GFF1877 at 551.287 kb on + strand at 551.287 kb on + strand at 551.295 kb on - strand at 551.539 kb on - strand, within GFF1878 at 551.550 kb on + strand, within GFF1878 at 551.550 kb on + strand, within GFF1878 at 551.551 kb on - strand, within GFF1878 at 551.756 kb on + strand at 551.756 kb on + strand at 551.756 kb on + strand at 551.757 kb on - strand at 551.757 kb on - strand at 551.757 kb on - strand at 551.757 kb on - strand at 551.757 kb on - strand at 551.774 kb on + strand at 551.775 kb on - strand at 551.775 kb on - strand at 551.775 kb on - strand at 551.775 kb on - strand at 551.837 kb on + strand at 551.837 kb on + strand at 551.837 kb on + strand at 551.838 kb on - strand at 551.838 kb on - strand at 551.838 kb on - strand at 551.843 kb on + strand at 551.843 kb on + strand at 551.844 kb on - strand at 551.844 kb on - strand at 551.844 kb on - strand at 551.844 kb on - strand at 551.844 kb on - strand at 551.844 kb on - strand at 551.849 kb on + strand at 551.849 kb on + strand at 551.850 kb on - strand at 551.850 kb on - strand at 551.850 kb on - strand at 551.850 kb on - strand at 551.889 kb on - strand at 551.889 kb on - strand at 552.002 kb on + strand, within GFF1879 at 552.002 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.185 kb on + strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.186 kb on - strand, within GFF1879 at 552.267 kb on - strand, within GFF1879 at 552.267 kb on - strand, within GFF1879 at 552.267 kb on - strand, within GFF1879 at 552.267 kb on - strand, within GFF1879 at 552.267 kb on - strand, within GFF1879 at 552.267 kb on - strand, within GFF1879 at 552.267 kb on - strand, within GFF1879 at 552.267 kb on - strand, within GFF1879 at 552.308 kb on + strand, within GFF1879 at 552.566 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.717 kb on - strand, within GFF1879 at 552.717 kb on - strand, within GFF1879
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 550,335 + GFF1876 0.83 -0.3 550,335 + GFF1876 0.83 -1.3 550,335 + GFF1876 0.83 -0.5 550,335 + GFF1876 0.83 -0.1 550,336 - GFF1876 0.83 +0.0 550,336 - GFF1876 0.83 -0.0 550,336 - GFF1876 0.83 -0.2 550,336 - GFF1876 0.83 -0.7 550,490 + -1.0 550,490 + -1.3 550,490 + -0.3 550,491 - -0.7 550,951 - GFF1877 0.58 -0.1 551,287 + -0.2 551,287 + +0.2 551,295 - -0.3 551,539 - GFF1878 0.55 -0.7 551,550 + GFF1878 0.58 +0.8 551,550 + GFF1878 0.58 -0.3 551,551 - GFF1878 0.58 -0.1 551,756 + -0.1 551,756 + +0.2 551,756 + -0.7 551,757 - -0.1 551,757 - -1.0 551,757 - +0.1 551,757 - +0.1 551,757 - +1.6 551,774 + -0.6 551,775 - -0.9 551,775 - -0.7 551,775 - -0.7 551,775 - +0.0 551,837 + +0.2 551,837 + +1.0 551,837 + -0.0 551,838 - -1.0 551,838 - -1.4 551,838 - -1.6 551,843 + -1.1 551,843 + -0.4 551,844 - +0.0 551,844 - +0.2 551,844 - -1.0 551,844 - +0.2 551,844 - -0.8 551,844 - +0.2 551,849 + +0.3 551,849 + +0.9 551,850 - -0.1 551,850 - +0.3 551,850 - +0.6 551,850 - -0.2 551,889 - +0.3 551,889 - -0.7 552,002 + GFF1879 0.18 -0.6 552,002 + GFF1879 0.18 -0.0 552,185 + GFF1879 0.32 -1.9 552,185 + GFF1879 0.32 -1.7 552,185 + GFF1879 0.32 -0.1 552,185 + GFF1879 0.32 +2.4 552,185 + GFF1879 0.32 -0.0 552,185 + GFF1879 0.32 -0.3 552,185 + GFF1879 0.32 +0.6 552,185 + GFF1879 0.32 -0.1 552,185 + GFF1879 0.32 -0.3 552,185 + GFF1879 0.32 +1.0 552,185 + GFF1879 0.32 -0.3 552,186 - GFF1879 0.32 +1.6 552,186 - GFF1879 0.32 +0.4 552,186 - GFF1879 0.32 -0.1 552,186 - GFF1879 0.32 +0.1 552,186 - GFF1879 0.32 +0.3 552,186 - GFF1879 0.32 +0.2 552,186 - GFF1879 0.32 -0.9 552,186 - GFF1879 0.32 +0.8 552,186 - GFF1879 0.32 -0.8 552,186 - GFF1879 0.32 +0.5 552,186 - GFF1879 0.32 -0.3 552,186 - GFF1879 0.32 -0.3 552,186 - GFF1879 0.32 -1.4 552,267 - GFF1879 0.38 -0.6 552,267 - GFF1879 0.38 -1.8 552,267 - GFF1879 0.38 +0.3 552,267 - GFF1879 0.38 +0.6 552,267 - GFF1879 0.38 +0.1 552,267 - GFF1879 0.38 +1.9 552,267 - GFF1879 0.38 -1.0 552,267 - GFF1879 0.38 -2.0 552,308 + GFF1879 0.41 +0.2 552,566 + GFF1879 0.60 +1.2 552,620 + GFF1879 0.64 -0.2 552,620 + GFF1879 0.64 -1.0 552,620 + GFF1879 0.64 -1.0 552,620 + GFF1879 0.64 +1.0 552,620 + GFF1879 0.64 +0.3 552,620 + GFF1879 0.64 +0.4 552,620 + GFF1879 0.64 -0.3 552,621 - GFF1879 0.64 +0.2 552,621 - GFF1879 0.64 -0.1 552,621 - GFF1879 0.64 -0.1 552,621 - GFF1879 0.64 -1.1 552,621 - GFF1879 0.64 +0.4 552,717 - GFF1879 0.71 +0.3 552,717 - GFF1879 0.71 -0.8
Or see this region's nucleotide sequence