Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1700 and GFF1701 are separated by 92 nucleotides GFF1701 and GFF1702 are separated by 170 nucleotides
GFF1700 - hypothetical protein, at 346,188 to 347,210
GFF1700
GFF1701 - BUG/TctC family periplasmic protein, at 347,303 to 348,307
GFF1701
GFF1702 - hypothetical protein, at 348,478 to 349,164
GFF1702
Position (kb)
347
348
349 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 346.383 kb on + strand, within GFF1700 at 346.523 kb on + strand, within GFF1700 at 346.525 kb on + strand, within GFF1700 at 346.525 kb on + strand, within GFF1700 at 346.525 kb on + strand, within GFF1700 at 346.526 kb on - strand, within GFF1700 at 346.526 kb on - strand, within GFF1700 at 346.526 kb on - strand, within GFF1700 at 346.526 kb on - strand, within GFF1700 at 346.526 kb on - strand, within GFF1700 at 346.526 kb on - strand, within GFF1700 at 346.526 kb on - strand, within GFF1700 at 346.765 kb on + strand, within GFF1700 at 346.766 kb on - strand, within GFF1700 at 346.766 kb on - strand, within GFF1700 at 346.783 kb on + strand, within GFF1700 at 346.783 kb on + strand, within GFF1700 at 346.783 kb on + strand, within GFF1700 at 346.784 kb on - strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.972 kb on + strand, within GFF1700 at 346.973 kb on - strand, within GFF1700 at 346.973 kb on - strand, within GFF1700 at 346.973 kb on - strand, within GFF1700 at 346.973 kb on - strand, within GFF1700 at 346.973 kb on - strand, within GFF1700 at 346.973 kb on - strand, within GFF1700 at 346.973 kb on - strand, within GFF1700 at 346.973 kb on - strand, within GFF1700 at 346.973 kb on - strand, within GFF1700 at 346.981 kb on + strand, within GFF1700 at 346.982 kb on - strand, within GFF1700 at 346.999 kb on + strand, within GFF1700 at 346.999 kb on + strand, within GFF1700 at 347.000 kb on - strand, within GFF1700 at 347.083 kb on + strand, within GFF1700 at 347.122 kb on + strand at 347.122 kb on + strand at 347.122 kb on + strand at 347.122 kb on + strand at 347.122 kb on + strand at 347.122 kb on + strand at 347.122 kb on + strand at 347.122 kb on + strand at 347.122 kb on + strand at 347.123 kb on - strand at 347.123 kb on - strand at 347.123 kb on - strand at 347.123 kb on - strand at 347.123 kb on - strand at 347.123 kb on - strand at 347.123 kb on - strand at 347.164 kb on + strand at 347.164 kb on + strand at 347.164 kb on + strand at 347.165 kb on - strand at 347.165 kb on - strand at 347.165 kb on - strand at 347.165 kb on - strand at 347.309 kb on + strand at 347.310 kb on - strand at 347.310 kb on - strand at 347.310 kb on - strand at 347.310 kb on - strand at 347.310 kb on - strand at 347.724 kb on + strand, within GFF1701 at 347.724 kb on + strand, within GFF1701 at 347.725 kb on - strand, within GFF1701 at 347.725 kb on - strand, within GFF1701 at 347.725 kb on - strand, within GFF1701 at 347.725 kb on - strand, within GFF1701 at 347.725 kb on - strand, within GFF1701 at 347.811 kb on + strand, within GFF1701 at 347.812 kb on - strand, within GFF1701 at 347.812 kb on - strand, within GFF1701 at 347.812 kb on - strand, within GFF1701 at 347.812 kb on - strand, within GFF1701 at 347.841 kb on + strand, within GFF1701 at 347.841 kb on + strand, within GFF1701 at 347.842 kb on - strand, within GFF1701 at 347.842 kb on - strand, within GFF1701 at 347.895 kb on + strand, within GFF1701 at 347.895 kb on + strand, within GFF1701 at 347.896 kb on - strand, within GFF1701 at 347.896 kb on - strand, within GFF1701 at 347.896 kb on - strand, within GFF1701 at 347.896 kb on - strand, within GFF1701 at 347.949 kb on + strand, within GFF1701 at 347.949 kb on + strand, within GFF1701 at 347.950 kb on - strand, within GFF1701 at 347.965 kb on - strand, within GFF1701 at 347.965 kb on - strand, within GFF1701 at 347.965 kb on - strand, within GFF1701 at 348.013 kb on + strand, within GFF1701 at 348.014 kb on - strand, within GFF1701 at 348.014 kb on - strand, within GFF1701 at 348.015 kb on + strand, within GFF1701 at 348.015 kb on + strand, within GFF1701 at 348.015 kb on + strand, within GFF1701 at 348.015 kb on + strand, within GFF1701 at 348.015 kb on + strand, within GFF1701 at 348.016 kb on - strand, within GFF1701 at 348.016 kb on - strand, within GFF1701 at 348.465 kb on + strand at 348.465 kb on + strand at 348.466 kb on - strand at 348.466 kb on - strand at 348.466 kb on - strand at 348.467 kb on + strand at 348.467 kb on + strand at 348.467 kb on + strand at 348.468 kb on - strand at 348.468 kb on - strand at 348.468 kb on - strand at 348.468 kb on - strand at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.751 kb on + strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 348.752 kb on - strand, within GFF1702 at 349.162 kb on + strand at 349.162 kb on + strand at 349.162 kb on + strand at 349.163 kb on - strand at 349.297 kb on + strand at 349.297 kb on + strand at 349.297 kb on + strand at 349.297 kb on + strand at 349.297 kb on + strand at 349.297 kb on + strand at 349.297 kb on + strand at 349.297 kb on + strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.298 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 346,383 + GFF1700 0.19 -0.1 346,523 + GFF1700 0.33 -0.3 346,525 + GFF1700 0.33 -0.6 346,525 + GFF1700 0.33 +0.0 346,525 + GFF1700 0.33 +0.4 346,526 - GFF1700 0.33 +0.2 346,526 - GFF1700 0.33 -0.1 346,526 - GFF1700 0.33 -0.8 346,526 - GFF1700 0.33 -0.3 346,526 - GFF1700 0.33 +0.4 346,526 - GFF1700 0.33 -0.4 346,526 - GFF1700 0.33 +0.1 346,765 + GFF1700 0.56 -1.2 346,766 - GFF1700 0.57 -0.5 346,766 - GFF1700 0.57 +0.6 346,783 + GFF1700 0.58 +0.3 346,783 + GFF1700 0.58 -0.4 346,783 + GFF1700 0.58 +0.1 346,784 - GFF1700 0.58 +0.0 346,972 + GFF1700 0.77 -0.3 346,972 + GFF1700 0.77 -0.9 346,972 + GFF1700 0.77 +0.6 346,972 + GFF1700 0.77 +0.1 346,972 + GFF1700 0.77 +0.2 346,972 + GFF1700 0.77 -1.3 346,972 + GFF1700 0.77 +0.1 346,972 + GFF1700 0.77 +0.6 346,972 + GFF1700 0.77 -0.4 346,972 + GFF1700 0.77 -1.5 346,972 + GFF1700 0.77 +0.2 346,972 + GFF1700 0.77 +0.8 346,972 + GFF1700 0.77 -0.9 346,972 + GFF1700 0.77 +0.4 346,972 + GFF1700 0.77 -0.2 346,972 + GFF1700 0.77 +0.6 346,972 + GFF1700 0.77 -0.4 346,972 + GFF1700 0.77 -0.6 346,973 - GFF1700 0.77 -0.5 346,973 - GFF1700 0.77 +0.7 346,973 - GFF1700 0.77 -0.3 346,973 - GFF1700 0.77 -0.4 346,973 - GFF1700 0.77 +0.1 346,973 - GFF1700 0.77 -0.5 346,973 - GFF1700 0.77 +0.6 346,973 - GFF1700 0.77 +0.9 346,973 - GFF1700 0.77 +0.5 346,981 + GFF1700 0.78 +0.1 346,982 - GFF1700 0.78 -1.2 346,999 + GFF1700 0.79 +0.5 346,999 + GFF1700 0.79 -0.3 347,000 - GFF1700 0.79 +3.4 347,083 + GFF1700 0.87 +0.2 347,122 + +0.5 347,122 + -0.9 347,122 + +0.4 347,122 + -2.5 347,122 + +0.7 347,122 + +0.5 347,122 + -0.4 347,122 + -0.1 347,122 + -0.3 347,123 - -0.3 347,123 - +0.4 347,123 - +0.1 347,123 - -0.5 347,123 - +1.3 347,123 - -1.8 347,123 - -0.6 347,164 + -0.0 347,164 + -1.0 347,164 + -0.6 347,165 - +0.7 347,165 - +0.3 347,165 - -0.3 347,165 - -0.3 347,309 + -1.6 347,310 - +0.6 347,310 - +0.1 347,310 - -1.1 347,310 - +0.1 347,310 - +1.5 347,724 + GFF1701 0.42 +0.0 347,724 + GFF1701 0.42 -0.4 347,725 - GFF1701 0.42 -1.2 347,725 - GFF1701 0.42 -0.4 347,725 - GFF1701 0.42 -1.0 347,725 - GFF1701 0.42 +0.7 347,725 - GFF1701 0.42 -1.4 347,811 + GFF1701 0.51 +1.3 347,812 - GFF1701 0.51 +0.2 347,812 - GFF1701 0.51 -0.3 347,812 - GFF1701 0.51 +0.4 347,812 - GFF1701 0.51 -1.3 347,841 + GFF1701 0.54 +0.5 347,841 + GFF1701 0.54 -0.4 347,842 - GFF1701 0.54 -0.5 347,842 - GFF1701 0.54 -2.3 347,895 + GFF1701 0.59 -1.8 347,895 + GFF1701 0.59 -1.0 347,896 - GFF1701 0.59 +0.8 347,896 - GFF1701 0.59 +0.6 347,896 - GFF1701 0.59 -1.1 347,896 - GFF1701 0.59 -0.8 347,949 + GFF1701 0.64 +0.1 347,949 + GFF1701 0.64 -0.4 347,950 - GFF1701 0.64 -0.3 347,965 - GFF1701 0.66 -0.6 347,965 - GFF1701 0.66 -1.2 347,965 - GFF1701 0.66 -0.8 348,013 + GFF1701 0.71 -0.5 348,014 - GFF1701 0.71 +0.3 348,014 - GFF1701 0.71 -0.5 348,015 + GFF1701 0.71 +1.4 348,015 + GFF1701 0.71 -0.8 348,015 + GFF1701 0.71 -1.0 348,015 + GFF1701 0.71 -3.2 348,015 + GFF1701 0.71 -0.5 348,016 - GFF1701 0.71 -0.1 348,016 - GFF1701 0.71 -0.5 348,465 + +0.3 348,465 + +2.1 348,466 - -0.3 348,466 - +0.7 348,466 - -0.7 348,467 + +0.3 348,467 + -0.5 348,467 + +0.6 348,468 - +0.7 348,468 - -0.1 348,468 - -0.3 348,468 - +0.2 348,751 + GFF1702 0.40 -0.7 348,751 + GFF1702 0.40 +1.1 348,751 + GFF1702 0.40 -1.3 348,751 + GFF1702 0.40 -1.2 348,751 + GFF1702 0.40 +0.1 348,751 + GFF1702 0.40 -1.0 348,751 + GFF1702 0.40 +0.4 348,751 + GFF1702 0.40 +0.1 348,751 + GFF1702 0.40 -0.5 348,751 + GFF1702 0.40 -0.2 348,751 + GFF1702 0.40 +0.3 348,751 + GFF1702 0.40 -0.8 348,751 + GFF1702 0.40 -1.3 348,752 - GFF1702 0.40 +0.2 348,752 - GFF1702 0.40 -0.5 348,752 - GFF1702 0.40 +0.1 348,752 - GFF1702 0.40 +0.2 348,752 - GFF1702 0.40 +0.1 348,752 - GFF1702 0.40 +1.1 348,752 - GFF1702 0.40 +0.5 348,752 - GFF1702 0.40 +0.7 348,752 - GFF1702 0.40 -0.5 348,752 - GFF1702 0.40 +0.5 349,162 + -1.6 349,162 + +0.6 349,162 + -0.7 349,163 - +0.6 349,297 + -0.9 349,297 + +1.4 349,297 + -0.3 349,297 + -1.5 349,297 + -0.1 349,297 + -0.0 349,297 + -0.1 349,297 + +0.3 349,298 - -0.3 349,298 - -1.3 349,298 - +0.7 349,298 - -1.1 349,298 - +1.1 349,298 - +1.1
Or see this region's nucleotide sequence