Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1685 and GFF1686 are separated by 26 nucleotides GFF1686 and GFF1687 overlap by 4 nucleotides GFF1687 and GFF1688 are separated by 19 nucleotides
GFF1685 - BUG/TctC family periplasmic protein, at 329,622 to 330,620
GFF1685
GFF1686 - Uncharacterized protein Bsu YraM, at 330,647 to 331,819
GFF1686
GFF1687 - BUG/TctC family periplasmic protein, at 331,816 to 332,793
GFF1687
GFF1688 - FIG00828468: hypothetical protein, at 332,813 to 334,240
GFF1688
Position (kb) 330
331
332 Strain fitness (log2 ratio) -2
-1
0
1
2 at 329.919 kb on + strand, within GFF1685 at 329.919 kb on + strand, within GFF1685 at 329.920 kb on - strand, within GFF1685 at 330.091 kb on - strand, within GFF1685 at 330.205 kb on - strand, within GFF1685 at 330.291 kb on + strand, within GFF1685 at 330.292 kb on - strand, within GFF1685 at 330.402 kb on - strand, within GFF1685 at 330.894 kb on - strand, within GFF1686 at 330.894 kb on - strand, within GFF1686 at 330.894 kb on - strand, within GFF1686 at 331.034 kb on + strand, within GFF1686 at 331.035 kb on - strand, within GFF1686 at 331.035 kb on - strand, within GFF1686 at 331.793 kb on + strand at 331.793 kb on + strand at 331.793 kb on + strand at 331.793 kb on + strand at 331.794 kb on - strand at 331.794 kb on - strand at 331.794 kb on - strand at 331.872 kb on + strand at 331.969 kb on + strand, within GFF1687 at 331.969 kb on + strand, within GFF1687 at 331.970 kb on - strand, within GFF1687 at 332.131 kb on + strand, within GFF1687 at 332.255 kb on - strand, within GFF1687 at 332.372 kb on - strand, within GFF1687 at 332.372 kb on - strand, within GFF1687
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 329,919 + GFF1685 0.30 +0.3 329,919 + GFF1685 0.30 +0.3 329,920 - GFF1685 0.30 -0.7 330,091 - GFF1685 0.47 -1.7 330,205 - GFF1685 0.58 -0.2 330,291 + GFF1685 0.67 -0.3 330,292 - GFF1685 0.67 +0.0 330,402 - GFF1685 0.78 -0.7 330,894 - GFF1686 0.21 -2.0 330,894 - GFF1686 0.21 -2.3 330,894 - GFF1686 0.21 -0.1 331,034 + GFF1686 0.33 -0.6 331,035 - GFF1686 0.33 -0.8 331,035 - GFF1686 0.33 +0.1 331,793 + -0.7 331,793 + -0.1 331,793 + +0.5 331,793 + -0.6 331,794 - -0.1 331,794 - -0.7 331,794 - +1.7 331,872 + +0.5 331,969 + GFF1687 0.16 -0.4 331,969 + GFF1687 0.16 -0.4 331,970 - GFF1687 0.16 +0.3 332,131 + GFF1687 0.32 +2.1 332,255 - GFF1687 0.45 -0.5 332,372 - GFF1687 0.57 +0.2 332,372 - GFF1687 0.57 -0.0
Or see this region's nucleotide sequence