Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1542 and GFF1543 are separated by 3 nucleotides GFF1543 and GFF1544 are separated by 2 nucleotides GFF1544 and GFF1545 are separated by 2 nucleotides
GFF1542 - Anti-sigma B factor RsbT / Phosphoserine phosphatase RsbX (EC 3.1.3.3), at 139,176 to 140,171
GFF1542
GFF1543 - Putative SigmaB asociated two-component system sensor protein, at 140,175 to 141,053
GFF1543
GFF1544 - Two-component system sensor histidine kinase/response regulator hybrid, at 141,056 to 143,044
GFF1544
GFF1545 - Pyruvate dehydrogenase (quinone) (EC 1.2.5.1), at 143,047 to 144,765
GFF1545
Position (kb) 141
142
143
144 Strain fitness (log2 ratio) -2
-1
0
1
2 at 140.120 kb on + strand at 140.120 kb on + strand at 140.120 kb on + strand at 140.121 kb on - strand at 140.121 kb on - strand at 140.121 kb on - strand at 140.121 kb on - strand at 140.121 kb on - strand at 140.121 kb on - strand at 140.121 kb on - strand at 140.121 kb on - strand at 140.121 kb on - strand at 140.296 kb on - strand, within GFF1543 at 140.364 kb on + strand, within GFF1543 at 140.364 kb on + strand, within GFF1543 at 140.365 kb on - strand, within GFF1543 at 140.558 kb on + strand, within GFF1543 at 140.559 kb on - strand, within GFF1543 at 140.682 kb on + strand, within GFF1543 at 140.683 kb on - strand, within GFF1543 at 140.828 kb on + strand, within GFF1543 at 140.829 kb on - strand, within GFF1543 at 141.140 kb on + strand at 141.141 kb on - strand at 141.398 kb on + strand, within GFF1544 at 141.398 kb on + strand, within GFF1544 at 141.399 kb on - strand, within GFF1544 at 141.399 kb on - strand, within GFF1544 at 141.512 kb on + strand, within GFF1544 at 141.512 kb on + strand, within GFF1544 at 141.512 kb on + strand, within GFF1544 at 141.513 kb on - strand, within GFF1544 at 141.513 kb on - strand, within GFF1544 at 142.687 kb on - strand, within GFF1544 at 142.847 kb on + strand at 142.866 kb on + strand at 143.049 kb on - strand at 143.049 kb on - strand at 143.049 kb on - strand at 143.277 kb on - strand, within GFF1545 at 143.330 kb on + strand, within GFF1545 at 143.330 kb on + strand, within GFF1545 at 143.330 kb on + strand, within GFF1545 at 143.331 kb on - strand, within GFF1545 at 143.331 kb on - strand, within GFF1545 at 143.331 kb on - strand, within GFF1545 at 143.331 kb on - strand, within GFF1545 at 143.331 kb on - strand, within GFF1545 at 143.393 kb on + strand, within GFF1545 at 143.393 kb on + strand, within GFF1545 at 143.393 kb on + strand, within GFF1545 at 143.393 kb on + strand, within GFF1545 at 143.394 kb on - strand, within GFF1545 at 143.501 kb on + strand, within GFF1545 at 143.925 kb on - strand, within GFF1545
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 140,120 + +0.9 140,120 + -0.6 140,120 + -1.6 140,121 - +0.3 140,121 - +0.2 140,121 - -2.0 140,121 - -0.1 140,121 - +0.7 140,121 - -0.7 140,121 - -0.6 140,121 - -0.3 140,121 - +0.3 140,296 - GFF1543 0.14 -0.6 140,364 + GFF1543 0.22 -0.0 140,364 + GFF1543 0.22 -0.3 140,365 - GFF1543 0.22 -0.2 140,558 + GFF1543 0.44 +0.6 140,559 - GFF1543 0.44 +0.1 140,682 + GFF1543 0.58 -1.3 140,683 - GFF1543 0.58 +0.2 140,828 + GFF1543 0.74 -0.1 140,829 - GFF1543 0.74 -0.4 141,140 + -1.3 141,141 - +0.3 141,398 + GFF1544 0.17 -0.3 141,398 + GFF1544 0.17 +0.3 141,399 - GFF1544 0.17 +0.3 141,399 - GFF1544 0.17 -0.0 141,512 + GFF1544 0.23 -0.1 141,512 + GFF1544 0.23 -0.3 141,512 + GFF1544 0.23 -0.3 141,513 - GFF1544 0.23 -0.7 141,513 - GFF1544 0.23 +1.0 142,687 - GFF1544 0.82 +0.2 142,847 + -0.7 142,866 + -0.5 143,049 - -1.6 143,049 - +1.0 143,049 - -0.1 143,277 - GFF1545 0.13 +0.3 143,330 + GFF1545 0.16 +0.8 143,330 + GFF1545 0.16 -1.0 143,330 + GFF1545 0.16 -0.2 143,331 - GFF1545 0.17 -0.3 143,331 - GFF1545 0.17 -0.2 143,331 - GFF1545 0.17 +0.6 143,331 - GFF1545 0.17 +0.1 143,331 - GFF1545 0.17 -0.8 143,393 + GFF1545 0.20 +0.3 143,393 + GFF1545 0.20 -0.3 143,393 + GFF1545 0.20 -0.3 143,393 + GFF1545 0.20 +2.3 143,394 - GFF1545 0.20 +0.4 143,501 + GFF1545 0.26 -0.5 143,925 - GFF1545 0.51 -1.8
Or see this region's nucleotide sequence