Strain Fitness in Variovorax sp. SCN45 around GFF1425

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1423 and GFF1424 overlap by 4 nucleotidesGFF1424 and GFF1425 overlap by 1 nucleotidesGFF1425 and GFF1426 are separated by 10 nucleotidesGFF1426 and GFF1427 are separated by 7 nucleotides GFF1423 - Phenylacetate ABC transporter, permease protein 1, at 14,424 to 15,296 GFF1423 GFF1424 - Phenylacetate ABC transporter, permease protein 2, at 15,293 to 16,225 GFF1424 GFF1425 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 16,225 to 16,974 GFF1425 GFF1426 - Phenylacetate ABC transporter, ATP-binding protein 2, at 16,985 to 17,704 GFF1426 GFF1427 - Mandelate racemase (EC 5.1.2.2), at 17,712 to 18,788 GFF1427 Position (kb) 16 17Strain fitness (log2 ratio) -1 0 1 2at 15.292 kb on + strandat 15.292 kb on + strandat 15.293 kb on - strandat 15.764 kb on + strand, within GFF1424at 15.764 kb on + strand, within GFF1424at 15.765 kb on - strand, within GFF1424at 15.765 kb on - strand, within GFF1424at 15.765 kb on - strand, within GFF1424at 15.818 kb on + strand, within GFF1424at 15.818 kb on + strand, within GFF1424at 15.818 kb on + strand, within GFF1424at 15.818 kb on + strand, within GFF1424at 15.968 kb on + strand, within GFF1424at 15.968 kb on + strand, within GFF1424at 15.969 kb on - strand, within GFF1424at 15.969 kb on - strand, within GFF1424at 16.125 kb on - strand, within GFF1424at 16.125 kb on - strand, within GFF1424at 16.235 kb on + strandat 16.236 kb on - strandat 16.236 kb on - strandat 16.988 kb on + strandat 17.351 kb on + strand, within GFF1426at 17.352 kb on - strand, within GFF1426at 17.352 kb on - strand, within GFF1426at 17.352 kb on - strand, within GFF1426at 17.352 kb on - strand, within GFF1426at 17.612 kb on + strand, within GFF1426at 17.612 kb on + strand, within GFF1426at 17.612 kb on + strand, within GFF1426at 17.613 kb on - strand, within GFF1426at 17.690 kb on + strandat 17.690 kb on + strandat 17.822 kb on - strand, within GFF1427at 17.859 kb on + strand, within GFF1427at 17.859 kb on + strand, within GFF1427at 17.860 kb on - strand, within GFF1427at 17.860 kb on - strand, within GFF1427at 17.860 kb on - strand, within GFF1427at 17.872 kb on - strand, within GFF1427at 17.967 kb on + strand, within GFF1427at 17.968 kb on - strand, within GFF1427

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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15,292 + +0.7
15,292 + -1.5
15,293 - -1.4
15,764 + GFF1424 0.50 -1.2
15,764 + GFF1424 0.50 -0.8
15,765 - GFF1424 0.51 +0.6
15,765 - GFF1424 0.51 -0.9
15,765 - GFF1424 0.51 +0.5
15,818 + GFF1424 0.56 -0.8
15,818 + GFF1424 0.56 -0.6
15,818 + GFF1424 0.56 -0.3
15,818 + GFF1424 0.56 +1.7
15,968 + GFF1424 0.72 -0.3
15,968 + GFF1424 0.72 -0.2
15,969 - GFF1424 0.72 +0.7
15,969 - GFF1424 0.72 -0.2
16,125 - GFF1424 0.89 +0.2
16,125 - GFF1424 0.89 -0.5
16,235 + +0.5
16,236 - +0.6
16,236 - -0.5
16,988 + -0.2
17,351 + GFF1426 0.51 -0.1
17,352 - GFF1426 0.51 -0.1
17,352 - GFF1426 0.51 +0.5
17,352 - GFF1426 0.51 -0.9
17,352 - GFF1426 0.51 -0.0
17,612 + GFF1426 0.87 +0.2
17,612 + GFF1426 0.87 +0.8
17,612 + GFF1426 0.87 +0.3
17,613 - GFF1426 0.87 +0.1
17,690 + +1.3
17,690 + -0.9
17,822 - GFF1427 0.10 +0.1
17,859 + GFF1427 0.14 -0.3
17,859 + GFF1427 0.14 -0.0
17,860 - GFF1427 0.14 +2.0
17,860 - GFF1427 0.14 -0.9
17,860 - GFF1427 0.14 +0.4
17,872 - GFF1427 0.15 -0.5
17,967 + GFF1427 0.24 +0.0
17,968 - GFF1427 0.24 -0.5

Or see this region's nucleotide sequence