Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1345 and GFF1346 overlap by 1 nucleotides GFF1346 and GFF1347 are separated by 15 nucleotides GFF1347 and GFF1348 are separated by 28 nucleotides GFF1348 and GFF1349 are separated by 9 nucleotides GFF1349 and GFF1350 are separated by 24 nucleotides
GFF1345 - 3-methylmercaptopropionyl-CoA dehydrogenase (DmdC), at 688,824 to 690,677
GFF1345
GFF1346 - hypothetical protein, at 690,677 to 690,928
GFF1346
GFF1347 - Dabb, at 690,944 to 691,246
GFF1347
GFF1348 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 691,275 to 692,072
GFF1348
GFF1349 - Thioesterase, at 692,082 to 692,546
GFF1349
GFF1350 - Quinone oxidoreductase (EC 1.6.5.5), at 692,571 to 693,587
GFF1350
Position (kb) 691
692
693 Strain fitness (log2 ratio) -2
-1
0
1 at 690.456 kb on + strand, within GFF1345 at 690.456 kb on + strand, within GFF1345 at 690.456 kb on + strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.579 kb on + strand at 690.580 kb on - strand at 690.591 kb on + strand at 690.591 kb on + strand at 690.782 kb on + strand, within GFF1346 at 690.782 kb on + strand, within GFF1346 at 690.782 kb on + strand, within GFF1346 at 690.783 kb on - strand, within GFF1346 at 690.783 kb on - strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.926 kb on + strand at 691.109 kb on + strand, within GFF1347 at 691.109 kb on + strand, within GFF1347 at 691.110 kb on - strand, within GFF1347 at 691.110 kb on - strand, within GFF1347 at 691.110 kb on - strand, within GFF1347 at 691.161 kb on - strand, within GFF1347 at 691.235 kb on + strand at 691.235 kb on + strand at 691.235 kb on + strand at 691.236 kb on - strand at 691.650 kb on + strand, within GFF1348 at 691.684 kb on - strand, within GFF1348 at 691.773 kb on + strand, within GFF1348 at 691.774 kb on - strand, within GFF1348 at 691.774 kb on - strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 692.199 kb on + strand, within GFF1349 at 692.200 kb on - strand, within GFF1349 at 692.200 kb on - strand, within GFF1349 at 692.431 kb on - strand, within GFF1349 at 692.431 kb on - strand, within GFF1349 at 692.478 kb on + strand, within GFF1349 at 692.566 kb on + strand at 692.567 kb on - strand at 692.567 kb on - strand at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.715 kb on + strand, within GFF1350 at 692.715 kb on + strand, within GFF1350 at 692.715 kb on + strand, within GFF1350 at 692.716 kb on - strand, within GFF1350 at 692.716 kb on - strand, within GFF1350 at 692.748 kb on + strand, within GFF1350 at 692.748 kb on + strand, within GFF1350 at 692.749 kb on - strand, within GFF1350 at 692.826 kb on + strand, within GFF1350 at 692.826 kb on + strand, within GFF1350 at 692.826 kb on + strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.871 kb on + strand, within GFF1350 at 692.871 kb on + strand, within GFF1350 at 692.872 kb on - strand, within GFF1350 at 692.872 kb on - strand, within GFF1350 at 692.983 kb on - strand, within GFF1350
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 690,456 + GFF1345 0.88 -0.4 690,456 + GFF1345 0.88 -1.1 690,456 + GFF1345 0.88 +0.2 690,457 - GFF1345 0.88 -0.4 690,457 - GFF1345 0.88 -0.9 690,457 - GFF1345 0.88 -1.4 690,457 - GFF1345 0.88 +0.3 690,457 - GFF1345 0.88 +1.3 690,579 + +0.3 690,580 - -0.0 690,591 + -1.4 690,591 + +0.3 690,782 + GFF1346 0.42 +0.1 690,782 + GFF1346 0.42 +0.2 690,782 + GFF1346 0.42 -0.2 690,783 - GFF1346 0.42 +0.2 690,783 - GFF1346 0.42 -0.5 690,899 + GFF1346 0.88 +0.1 690,899 + GFF1346 0.88 +0.7 690,899 + GFF1346 0.88 -0.4 690,899 + GFF1346 0.88 +0.3 690,900 - GFF1346 0.88 +0.6 690,900 - GFF1346 0.88 +0.6 690,900 - GFF1346 0.88 -1.0 690,900 - GFF1346 0.88 -0.4 690,900 - GFF1346 0.88 -0.7 690,900 - GFF1346 0.88 -0.2 690,900 - GFF1346 0.88 +0.1 690,900 - GFF1346 0.88 +0.1 690,926 + -0.0 691,109 + GFF1347 0.54 +0.5 691,109 + GFF1347 0.54 +0.8 691,110 - GFF1347 0.55 -0.2 691,110 - GFF1347 0.55 +1.6 691,110 - GFF1347 0.55 -0.5 691,161 - GFF1347 0.72 +0.4 691,235 + -0.8 691,235 + -0.6 691,235 + -0.7 691,236 - -1.5 691,650 + GFF1348 0.47 -0.7 691,684 - GFF1348 0.51 -0.8 691,773 + GFF1348 0.62 +0.9 691,774 - GFF1348 0.63 -1.2 691,774 - GFF1348 0.63 -1.0 691,836 + GFF1348 0.70 -1.8 691,836 + GFF1348 0.70 -0.0 691,836 + GFF1348 0.70 -0.9 691,837 - GFF1348 0.70 +0.1 691,837 - GFF1348 0.70 -0.5 691,837 - GFF1348 0.70 -0.2 691,837 - GFF1348 0.70 -0.6 692,199 + GFF1349 0.25 +0.6 692,200 - GFF1349 0.25 -1.2 692,200 - GFF1349 0.25 +0.4 692,431 - GFF1349 0.75 -1.5 692,431 - GFF1349 0.75 -0.3 692,478 + GFF1349 0.85 +0.1 692,566 + -0.1 692,567 - +0.4 692,567 - -0.3 692,697 + GFF1350 0.12 -0.5 692,697 + GFF1350 0.12 -1.2 692,697 + GFF1350 0.12 +0.4 692,697 + GFF1350 0.12 +0.3 692,697 + GFF1350 0.12 +1.1 692,697 + GFF1350 0.12 -0.6 692,698 - GFF1350 0.12 +0.1 692,698 - GFF1350 0.12 -0.4 692,698 - GFF1350 0.12 -2.4 692,698 - GFF1350 0.12 +0.4 692,698 - GFF1350 0.12 +0.1 692,698 - GFF1350 0.12 +0.1 692,698 - GFF1350 0.12 -0.8 692,698 - GFF1350 0.12 +0.6 692,698 - GFF1350 0.12 -0.4 692,715 + GFF1350 0.14 -0.4 692,715 + GFF1350 0.14 -0.5 692,715 + GFF1350 0.14 +0.7 692,716 - GFF1350 0.14 -0.1 692,716 - GFF1350 0.14 -1.2 692,748 + GFF1350 0.17 +0.2 692,748 + GFF1350 0.17 +0.2 692,749 - GFF1350 0.18 +1.2 692,826 + GFF1350 0.25 -1.2 692,826 + GFF1350 0.25 +0.3 692,826 + GFF1350 0.25 -0.3 692,827 - GFF1350 0.25 -0.2 692,827 - GFF1350 0.25 -0.2 692,827 - GFF1350 0.25 -1.2 692,827 - GFF1350 0.25 -0.9 692,827 - GFF1350 0.25 -0.1 692,871 + GFF1350 0.29 -1.4 692,871 + GFF1350 0.29 -1.2 692,872 - GFF1350 0.30 -0.0 692,872 - GFF1350 0.30 +0.5 692,983 - GFF1350 0.41 +1.2
Or see this region's nucleotide sequence